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At the Bench: A Laboratory Navigator

Subject Area(s):  Laboratory Manuals/Handbooks

By Kathy Barker, Rockefeller University, New York

More INFO Below
Description
Reviews
Contents
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© 1998 • 460 pp., glossary, index
Concealed wire binding • ISBN  0-87969-523-4


 

Description

A research laboratory filled with competent, busy people entirely familiar with its arcane customs and practices is a daunting place for newcomers. Kathy Barker knows this world. She was a technician, an undergraduate, then a graduate student at the University of Massachusetts, and as a postdoctoral fellow and assistant professor at Rockefeller University, she was a mentor to grad students, physicians in training, technicians, and research nurses. From this rich experience, she has written At the Bench, a unique handbook for living and working in the laboratory. Much more than a simple primer or lab manual, this book is an essential aid to understanding:

  • how research groups work at a human level—and how to fit in
  • what equipment is essential, and how to use it properly
  • how to get started and get organized
  • how to set up an experiment
  • how to handle and use data and reference sources
  • how to present yourself and your results—in print and in person

Wise, light-hearted, but thoroughly practical, Dr. Barker offers advice, moral support, social etiquette, and professional reassurance along with assume-nothing, step-by-step instructions for those basic but vital laboratory procedures that experienced investigators know—but may not realize novices don’t.

If you are a graduate student, a physician with research intentions, or a laboratory technician, this book is indispensable. If you have to manage or mentor such people, giving a copy to each of them will greatly improve your life, and theirs.

 
 

Reviews

review:  “Dr. Barker has managed to distill the collective wisdom and intuitive flair of experimentalists of the last three decades into a survival kit no bench worker should be without. While the purpose of the book is to introduce the wonders and terrors of the lab to the uninitiated, the wealth of useful information contained in this volume make it a valuable addition for the more sophisticated bench scientist. I don't know of a single volume which provides everything from buffer recipes to half-lifes of common isotopes, principles of centrifugation to growth media for bacterial strains, to name but a few examples.

The volume follows the new lab worker from his first day on the job through the ins and outs of assimilating into the lab. Tips are provided on who runs the show (the secretary and senior tech) to how to get started (do an experiment the first week). The reader is instructed on how to make friends, when to speak up and when to hold back. While much of this advice is simple common sense, having it made explicit is no doubt reassuring and worthwhile. Dr. Barker makes it clear that the integrity of the data are paramount, as they should be, and this message is explicit. The majority of the volume gets down to the nuts and bolts and getting the experiments done, describing equipment (and providing delightful drawings labeling everything from the shaker to the HPLC), instructing on good technique and providing the kind of useful tables to make sure what needs to get autoclaved does and what doesn't is sterile filtered.”
      —JEFFREY V. RAVETH, M.D., Ph.D., Rockefeller University

review:  “Where was this book when ‘I’ started in the lab? Like the ‘(Computers, etc.)... for Dummies’ series in popular bookstores, this is an invaluable practical guide for working in a laboratory, and scientific life in general. It addresses questions the newcomer may not have thought of asking, and provides a ‘safe’ resource to find the answers to questions he or she may be reluctant to ask. For the established members of the lab, this manual relieves them of much of the basic training tasks for the newcomer, and provides a standardized body of knowledge everyone is expected to master; the latter also being important when establishing liability. Furthermore, this book provides a rare and insightful explanation of scientific culture, helping socialize the novice. Despite being written for beginners, this manual can serve as a timeless reference for the experienced scientist.”
      —ALAN R. SALTIEL, Ph.D., Parke-Davis Pharmaceuticals

review:  “While it is no substitute for training by a good supervisor, At the Bench is a well–organized reference that offers practical guidelines as well as basic protocols for students and technicians in their first months in the lab. It is full of useful illustrations and tables, some of which will be helpful even to veteran researchers. Best of all, it's concise—weighing in at about one kilogram—it's far less daunting a starting point than any multi–volume book of protocols.”
      —Trends in Cell Biology

review:  “On the whole, At the Bench is very easy to read and navigate around, and is very comprehensive. However, whether the students that might be helped by this book will receive it before starting in a laboratory is a different matter. The cost of the book makes it a bargain and I wonder whether supervisors should be encouraged to send copies to students in the summer before they start their research projects, instead of the large volume of tedious background reading that most of them receive.”
      —Trends in Neurosciences

review:  “I would highly recommend that every laboratory purchase this book and have it readily available for all personnel. It could save everyone a lot of time, reduce stress level, improve safety issues and create a more pleasant working environment for all.”
      —Trends in Genetics

review:  “All in all, the book would be a useful addition to any academic lab to form part of the induction process for new researchers.”
      —Microbiology Today

review:  “Barker's manual is appropriate for starting technicians, students (both undergraduate and graduate), as well as, for example, medical fellows entering a research lab for the first time. I thought it also useful for starting principal investigators “staring at headlights,” like I had been. Its clarity would have been eminently helpful to my thinking in fashioning my entry–level speech to new students, technicians, and fellows. I have also recently realized I can use the book even now, some 14 years into running a lab: I gave the book to an entering student, from whom it was returned with scribbles in the margins in places where he clearly learned something, or where a point he probably already knew was particularly well–phrased and, thus, more memorable.  This budding scientist had been a technician before and had already been introduced to the usual lab culture. Yet, now he was embarking along a distinct path within that culture and still found items within Barker's book quite useful ('think of every experiment as a publishable unit' was one underlined phrase).”
      —Cell Biology Education

review:  “Kathy Barker has done an excellent job in presenting the many aspects of laboratory work in this book from Cold Spring Harbor Laboratory Press. At the Bench is not a textbook or manual but rather a lighthearted guide to working in a lab. Readers are walked step–by–step through the basics of lab navigation, experimentation methods and specific procedures. The neophyte will appreciate all the detail that is available, especially the social aspects of the laboratory that can sometimes be difficult to decipher. Ms. Barker offers expert advice on lab etiquette including behavior, expectations, attitude, collaboration and confrontation.  Her advice is sensible, however it is general; interpersonal relations depend greatly on the personality and character of each individual within the lab. Throughout the text Ms. Barker provides a wide variety of resources for further research, but an emphasis is placed on finding details in lab resources, both the literature and the personnel...

This book is a necessity for newcomers to a laboratory setting with practical information and advice. It should become a staple for any laboratory bench with its emphasis on basic procedures, materials and techniques.”
      —Biomolecular Engineering

 
 Available by Special Request 
 

Contents

Preface
Abbreviations

SECTION 1. Getting Oriented

Chapter 1: General Lab Organization and Procedures
The Big Picture
Laboratory Personnel
Lab Routines
What to Expect the First Week
What to Do the First Week
What Not to Do the First Week
Survival Through Common Sense and Courtesy
Nonnegotiable Safety Rules
Resources
Chapter 2: Laboratory Setup and Equipment
Lay of the Land
Using the Equipment
How to Buy New Equipment
Resources
Chapter 3: Getting Started and Staying Organized
Setting Up a Functional Lab Bench
Setting Up the Command Center
Resources

SECTION 2. Plotting a Course

Chapter 4: How To Set Up an Experiment
Philosophical Considerations
Planning an Experiment
Interpreting Results
Resources
Chapter 5: Laboratory Notebooks
Type and Format
Content
Maintenance
Ethics
Resources
Chapter 6: Presenting Yourself and Your Data
Communication Tips
Oral Presentations
Written Presentations
Resources

SECTION 3. Navigating

Chapter 7: Making Reagents and Buffers
Determining What You Need
Calculating What You Need
Weighing and Mixing
Measuring pH
Sterilizing Solutions
Storing Buffers and Solutions
Resources
Chapter 8: Storage and Disposal
Emergency Storage
Storing Reagents
Aliquoting
Refrigerator and Freezers
Discarding Lab Waste
Resources
Chapter 9: Working without Contamination
When to Use Sterile Technique
Sterile Technique
Protecting the Investigator
Sterile Technique in the Class II Biosafety Cabinet
Resources
Chapter 10: Eukaryotic Cell Culture
Types of Cultures and Cell Lines
Observing Cells
Obtaining Cells
Cell Maintenance
Freezing and Storage of Cells
Contamination
CO2 Incubators and Tanks
Resources
Chapter 11: Bacteria
Setting Up
Working Rules
Obtaining Bacteria
Growth and Maintenance
Antibiotics
Reviving Cultures
Obtaining Isolated Colonies
Counting Bacteria
Storage
Freezing Bacteria
Contamination
Resources
Chapter 12: DNA, RNA, and Protein
Molecular Biology Tips
DNA
Introducting DNA into Cells and Microorganisms
RNA
Protein
Resources
Chapter 13: Radioactivity
Properties of Radioactive Elements
How to Obtain Radioisotopes
Doing Radioactive Experiments
Experimental Detection of Radiation
Storage
Disposal
Alternatives to Radioactivity
Resources
Chapter 14: Centrifugation
Background
Working Rules
How to Spin
Gradients
Centrifuge and Rotor Maintenance
Resources
Chapter 15: Electrophoresis
Basic Rules
Generalities
Specifics
Transferring Gel Contents to Membranes
Resources
Chapter 16: The Light Microscope
Background
Using the Light Microscope
Slides and Stains
Fluorescence Microscopy
Photography
Shared Instrument Facilities
Resources

Glossary
Index

 
 

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At the Bench: A Laboratory Navigator, Updated Edition [Concealed wire binding]
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