© 2002 • 682 pp., illus., appendices, index
Hardcover • $205 $41.00 • ISBN  0-87969-604-4
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As more genetic and biochemical information about the protein components of cells accumulate, the analysis of protein-protein interactions is becoming increasingly important. This manual presents a wide range of techniques for identifying and analyzing these interactions, starting with standard molecular and biochemical techniques, and progressing to biophysical and computational approaches and therapeutic and other post-genomic applications. This manual is designed to complement the information in the best-selling Molecular Cloning manual, and is presented in the same clear, user-friendly format. It is an essential resource for investigators studying interacting sets of proteins and their physiologic significance in a wide range of experimental systems.

  "This book develops an appreciation of the wide range of techniques addressing protein–protein interaction issues, and why some are better suited than others to answer a specific biological question."
      —Trends in Biotechnology

  "The 682 pages cover a broad range of very different techniques, from simple co–precipitation protocols to highly sophisticated microscopic and spectrometric techniques. . . . At the heart of the book are of course those chapters that provide protocols on how to perform a successful binding experiment or interaction screen. While being aware that one can truly judge the quality of these protocols only by executing them, I found most of them very carefully written and referenced, thus promising that they will indeed be helpful guides for the experimentalist. . . . The book deserves to land on the shelves of many labs and offices. In this new age of proteomics, it might even become a 'must have'."
      —Trends in Cell Biology

  "Protein–Protein Interactions is not just a collection of experimental protocols. In addition to the methods chapters one finds more general, review–like chapters which address selected topics such as areas of cell biology in which protein interaction experiments have led to deeper insight into a cell's molecular machinery or new or attempted applications in the pharmacological environment, for example. These chapters provide some interesting ideas on how and to which ends protein interaction studies may be used in the future . . .

Protein–Protein Interactions lives up to the expectations which users who have used other Cold Spring Harbor manuals will have. It is a valid addition to the CSHL Press's catalogue of standard–:setting laboratory manuals.  Potential buyers are strongly advised to purchase the hardcover edition which is much better suited to survive the wear and tear of the frequent use that this book is likely to encounter in its 'workplace.'"
      —Proteomics

Chapter 1: Toward an Understanding of Protein Interactions, E. Golemis

Section One: The Biological Context for Protein Interaction Studies: Physiological Indicators of Protein-Protein Interaction

Chapter 2: Signal Transduction and Mammalian Cell Growth: Problems and Paradigms, J. Chernoff

Chapter 3: Impact of Protein Interaction Technologies on Cancer Biology and Pharmacogenomics, R.B. Raftogianis and A.K. Godwin

Section Two: Standard Technologies Used to Identify and Characterize Protein-Protein Interactions

Chapter 4: Identification of Protein-Protein Interactions with Glutathione-S-Transferase Fusion Proteins, M.B. Einarson and J.R. Orlinick

Chapter 5: Identification of Associated Proteins by Coimmunoprecipitation, P.D. Adams, S. Seeholzer, and M. Ohh

Chapter 6: Chemical Cross-linking in Studying Protein-Protein Interactions, O.W. Nadeau and G.M. Carlson

Chapter 7: Yeast and Bacterial Two-hybrid Selection Systems for Studying Protein-Protein Interactions, I. Serebriiskii and J.K. Joung

Chapter 8: Phage-display Approaches for the Study of Protein-Protein Interactions, C.S. Goodyear and G.J. Silverman

Chapter 9: Genetic Approaches to Identify Protein-Protein Interactions in Budding Yeast, R. Strich

Section Three: Biophysical Approaches to Identify and Characterize Protein-Protein Interactions

Chapter 10: Imaging Protein Interactions by FRET Microscopy, P.J. Verveer, A.G. Harpur, and P.I.H. Bastiaens

Chapter 11: Detection of Homotypic Protein Interactions with Green Fluorescent Protein Proximity Imaging (GFP-PRIM), D.A. De Angelis

Chapter 12: Characterization of Multiprotein Complexes by Mass Spectrometry, C.V. Robinson

Chapter 13: Probing Ligand-Receptor Interactions with Atomic Force Microscopy, X. Zhang, A. Chen, E. Wojcikiewicz, and V.T. Moy

Chapter 14: Analysis of Interacting Proteins with Surface Plasmon Resonance Using Biacore, M.V. Medaglia and R.J. Fisher

Chapter 15: Analysis of Protein Interactions Using a Quartz Crystal Microbalance Biosensor, S. Hauck, S. Drost, E. Prohaska, H. Wolf, and S. Dübel

Section Four: Recent Developments and Other Tools: Reviews and Short Protocols

Chapter 16: Protease Footprinting, R. Hori and N. Baichoo

Chapter 17: Tandem Affinity Purification to Enhance Interacting Protein Identification, B. Séraphin, O. Puig, E. Bouveret, B. Rutz, F. Caspary

Chapter 18: Protein Purification by Inverse Transition Cycling, D.E. Meyer and A. Chilkoti

Chapter 19: Using In Vitro Expression Cloning to Identify Interacting Proteins, F. Kanai, M.B. Yaffe, and P.T. Stukenberg

Chapter 20: Using Egg Extracts to Modify Recombinant Proteins, B. Spodik, S.H. Seeholzer, and T.R. Coleman

Chapter 21: Using lambda Repressor Fusions to Isolate and Characterize Self-assembling Domains, L. Mariño-Ramirez and J.C. Hu

Chapter 22: The Membrane-based Yeast Two-hybrid System, S. Thaminy and I. Stagljar

Chapter 23: Protein Interactions in Live Cells Monitored by beta-Galactosidase Complementation, B.T. Blakely, F.M.V. Rossi, T.S. Wehrman, C.A. Charlton, and H.M. Blau

Chapter 24: Identification of Protein Single-chain Antibody Interactions In Vivo Using Two-hybrid Protocols, A. Pörtner-Taliana, K.F. Froning, I. Kusser, and M. Russell

Chapter 25: Protein Interactions and Library Screening with Protein Fragment Complementation Strategies, I. Remy, J.N. Pelletier, A. Galarneau, S.W. Michnick

Chapter 26: A Bacterial Two-hybrid System Based on a Cyclic AMP Signaling Cascade, G. Karimova, A. Ullmann, and D. Ladant

Chapter 27: Peptide Aptamers to Study Proteins and Protein Networks, R.L. Finley, Jr., A.H. Soans, and C.A. Stanyon

Chapter 28: Generation of Protein Fragment Libraries by Incremental Truncation, M. Ostermeier, S. Lutz, and S.J. Benkovic

Chapter 29: Catalytic Antibodies: New Characters in the Protein Repertoire, G.A. Nevinsky, O.O. Favorova, and V.N. Buneva

Chapter 30: In Vitro Selection and Evolution of Protein-Ligand Interactions by Ribosome Display, C. Schaffitzel, C. Zahnd, P. Amstutz, B. Luginbühl, and A. Plückthun

Chapter 31: Analysis of Protein Interactions with Peptides Synthesized on Membranes, J. Koch, M. Mahler, M. Blüthner, and S. Dübel

Chapter 32: Protein Bundling to Enhance the Detection of Protein-Protein Interactions, S. Natesan

Section Five: Protein-Protein Interactions: Computation and the Future

Chapter 33: Analysis of Genome-wide Protein Interactions Using Computational Approaches, A.J. Enright and C.A. Ouzounis

Chapter 34: Visualization and Integration of Protein-Protein Interactions, P. Uetz, T. Ideker, and B. Schwikowski

Chapter 35: Modulating Protein-Protein Interactions to Develop New Therapeutic Approaches, V. Jérôme and R. Müller