© 2003 • 712 pp., illus., appendices, index
Paperback • ISBN  978-087969625-2

This title also available in: Hardcover

DNA microarray technology is a new and powerful means to analyze genomes and characterize patterns of gene expression. Its applications are widespread across the many fields of plant and animal biological and biomedical research. This manual, designed to extend and to complement the information in the best–selling Molecular Cloning, is a synthesis of the expertise and experience of more than 30 contributors—all innovators in a fast–moving field. DNA Microarrays provides authoritative, detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for analysis of images and data.

  “DNA Microarrays: A Molecular Cloning Manual provides the reader with a wealth of information about how to manufacture microarrays, design and carry out microarray experiments, and analyze microarray data.  The editors have lined up an impressive cast of authors who are in many cases the world experts on the particular aspect of microarrays technology that they describe. . . . Overall, this is an excellent book, which will prove invaluable to any researcher involved in microarray analysis. It offers a comprehensive overview of the microarray technology as well as its satellite techniques, such as LCM, CGH arrays, and tissue arrays, and is the most comprehensive and up–to–date manual currently available on DNA microarrays.”
      —Journal of Proteome Research

  “DNA Microarrays is a welcome addition to the library of anyone using microarrays. In the tradition of Cold Spring Harbor manuals, this volume provides detailed protocols for every aspect of the technology, from microarray fabrication to data analysis. The protocols cull the expertise of top microarray researchers and cover both expression profiling and analysis of genomic DNA. . . . Overall, the protocols are comprehensive and sufficiently detailed for newcomers to microarrays, and the book contains enough pearls to ensure that it will aslo be welcomed in experienced microarrays labs.”
      —Nature Cell Biology

SECTION 1: GENERATION OF PROBES FOR SPOTTED MICROARRAYS

S. Chandrasekharappa, A. Holloway, V. Iyer, D. Monte, M. Murphy, N.J. Nowak

SECTION 2: PRINTING SPOTTED GLASS MICROARRAYS

G. Childs, J. DeRisi, T. Harris, A. Holloway, B.–H. Hou, A. Massimi, M. Murphy, S. Somerville

SECTION 3: EXPRESSION ANALYSIS OF RNA

M. Bittner, R. Butow, J. DeRisi, M. Diehn, J. Eberwine, C. Epstein, R. Glynne, S. Grimmond, T. Ideker, J.E. Kacharmina, S. Katsabanis, J. Khan, J. Lee, C.L. Liu, P. Marciano, F.M. Marincola, T. McIntosh, D. Monte, J.R. Pollack, V. Rhodius, S. Somerville, E. Tom, E. Wang, J.S. Wei, D. Willhite, S. Ybarra

SECTION 4: MEMBRANE–BASED SPOTTED cDNA ARRAYS

K. Becker, W.H. Wood III, C. Cheadle

SECTION 5: TISSUE MICRODISSECTION

B. Becker, M. Bernsen, C.J.M. Best, T. Björnsen, D. Brocksch, R. Burgemeister, C. Bush, R.F. Chuaqui, A. Clement–Sengewald, M.R. Emmert–Buck, G. Friedemann, J.W. Gillespie, W. Heckl, G. Lahr, I.M. Leiva, P. Lindahl, A. Mayer, S. Nilsson, S.J. Scheidl, K. Schütze, M. Stich, W. Stolz, M. Takemoto, S. Thalhammer, M.C.R.F. van Dijk, T. Vogt

SECTION 6: USING MICROARRAYS FOR GENOMIC ANALYSIS

D. Albertson, S. Dee, J.–B. Fan, J.N. Hirschhorn, X. Huang, V. Iyer, Y.–M. Lim, K. Lindblad?Toh, R. Lucito, H. Matsuzaki, N. Patil, D. Pinkel, J.R. Pollack, T. Ryder, R. Segraves, P. Sklar, A. Snijders, M. Wigler

SECTION 7: AN INTRODUCTION TO MICROARRAY BIOINFORMATICS

C.A. Ball, Y. Chen, S. Panavally, G. Sherlock, T. Speed, P.T. Spellman, Y.H. Yang

SECTION 8: TISSUE MICROARRAYS

K. Brand, G. Hostetter, O.–P. Kallioniemi, J. Kononen, G. Sauter, M. Trivett

APPENDIX 1: Generation of Full-length Libraries

P. Carninci

APPENDIX 2: Reagents, Buffers, and Media

APPENDIX 3: Cautions

APPENDIX 4: Suppliers