© 2011 • 517 pp., illus. (82 color, 17 b/w), index
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Hardcover • $135 33.75 • ISBN  978-0-879698-94-2
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The nucleus of a cell contains its DNA and is the site where DNA replication, transcription, and RNA processing take place. Nuclei have many domains but lack the membrane-bound organelles characteristic of the cytoplasm. Instead, nuclear bodies appear to dynamically self-organize, assembling and disassembling according to the functional demands of the cell.

Written and edited by experts in the field, this collection from Cold Srping Harbor Perspectives in Biology includes contributions discussing the relationship between nuclear structure and function, the various nuclear bodies that have been identified, and the organization of the nuclear lamina and nuclear pore complex. Other contributions examine the higher-order organization of chromatin within the nucleus and the dynamics of DNA replication, transcription, DNA repair, and RNA transport.

Including a historical introduction to the field and discussion of the numerous pathological conditions involving disruption of nuclear structure and function, this volume is essential reading for all molecular and cell biologists, as well as pathologists interested in the role of nuclear architecture in disease.

  “This book is a ‘must read’ for anyone working on nuclear dynamics, serving as general background in a cover-to-cover reading or as a reference with citations of the pertinent literature for a given subject. This book is a nice sequel to the Cold Spring Harbor Symposium volume on the same subject...this book is highly recommended as worth having on one's bookshelf. It is a valuable resource.”
      —Nucleus

  “The 28 chapters are organized to provide a comprehensive overview of nuclear compartments and their components (e.g. nuclear membrane, nuclear pore complex, chromosome territories, Cajal body, nucleolus), to discuss how this organization relates to function (transcription, DNA replication and repair, RNA processing) and to describe the defects in nuclear organization in human disease. The focus is primarily on the nucleus in mammalian cells, but there are also discussions on other simpler model organisms — budding yeast, Caenorhabditis elegans and Drosophila. Each chapter is relatively short and accessible, making this a great resource to dip into. Illustrations are used sparingly but are well chosen....if you interest in the nucleus is piqued, then this book is a good place to start.”
      —Development

  “...Misteli is one of the most creative and productive recent arrivals in the nucleus field, as is his former postdoc mentor, David Spector....every chapter in the book were written by undeniable world leaders....The coverage is complete in that there is no structure or function of the nucleus that does not have a chapter....the book has all of the hallmarks of the fine publishing house that is Cold Spring Harbor Laboratory Press....The result is a book about the nucleus that will not be surpassed anytime soon.”
      —The FASEB Journal

Preface

 

OVERVIEW

The Nucleus Introduced

Thoru Pederson

 

THE NUCLEAR PERIPHERY

The Nuclear Envelope

Martin W. Hetzer

Nuclear Lamins

Thomas Dechat, Stephen A. Adam, Pekka Taimen, Takeshi Shimi, and Robert D. Goldman

Lamin-binding Proteins

Katherine L. Wilson and Roland Foisner

The Nuclear Pore Complex and Nuclear Transport

Susan R. Wente and Michael P. Rout

 

CHROMOSOMES & CHROMATIN

Chromosome Territories

Thomas Cremer and Marion Cremer

Gene Positioning

Carmelo Ferrai, Inês Jesus de Castro, Liron Lavitas, Mita Chotalia, and Ana Pombo

Chromatin Higher-order Structure and Dynamics

Christopher L. Woodcock and Rajarshi P. Ghosh

Nuclear Organization and Dosage Compensation

Jennifer C. Chow and Edith Heard

The Budding Yeast Nucleus

Angela Taddei, Heiko Schober, and Susan M. Gasser

Nuclear Functions of Actin

Neus Visa and Piergiorgio Percipalle

 

NUCLEAR BODIES

The Nucleolus

Thoru Pederson

Nuclear Speckles

David L. Spector and Angus I. Lamond

The Cajal Body and Histone Locus Body

Zehra Nizami, Svetlana Deryusheva, and Joseph G. Gall

PML Nuclear Bodies

Valérie Lallemand-Breitenbach and Hugues de Thé

The Perinucleolar Compartment

Callie Pollock and Sui Huang

Paraspeckles

Archa H. Fox and Angus I. Lamond

Nuclear Stress Bodies

Giuseppe Biamonti and Claire Vourc’h

Orphan Nuclear Bodies

Maria Carmo-Fonseca, Maria T. Berciano, and Miguel Lafarga

Biogenesis of Nuclear Bodies

Miroslav Dundr and Tom Misteli

 

FUNCTIONAL ORGANIZATION

Organization of Transcription

Lyubomira Chakalova and Peter Fraser

Organization of DNA Replication

Vadim O. Chagin, Jeffrey H. Stear, and M. Cristina Cardoso

DNA Damage Response

Giuseppina Giglia-Mari, Angelika Zotter, and Wim Vermeulen

RNA Processing and Export

Sami Hocine, Robert H. Singer, and David Grünwald

 

NUCLEAR ARCHITECTURE IN DIFFERENTIATION AND DISEASE

Diseases of the Nuclear Envelope

Howard J. Worman, Cecilia Östlund, and Yuexia Wang

Higher-order Genome Organization in Human Disease

Tom Misteli

Nuclear Ataxias

Harry T. Orr

Developments in RNA Splicing and Disease

Michael G. Poulos, Ranjan Batra, Konstantinos Charizanis, and Maurice S. Swanson

 

APPENDIX

Tables of Data on the Nucleus

(Adapted by kind permission of Federation of American Societies for Experimental Biology [FASEB] from Biological Handbooks. I. Cell Biology [Altman P.L. and Katz DD; 1976], Bethesda, Maryland.)

 

Index