© 2011 • 322 pp., illus. (56 4C and 12 B&W images), index
Hardcover • $135 • ISBN  978-0-87969-873-7

Identified more than 100 years ago by Camillo Golgi, the Golgi is a series of flattened cisternae that acts as a critical sorting and processing station for intracellular traffic. It receives cargo in vesicles exported from the endoplasmic reticulum and then dispatches this to the plasma membrane for secretion or directs it to other organelles, such as endosomes. The Golgi also plays an important role in carbohydrate metabolism: It is the major site for attachment of sugar moieties to proteins.

Written and edited by experts in the field, this volume includes contributions that review the current models for Golgi traffic and detail the functions of the cargo carrying machinery, such as the COPI and COPII vesicles, the glycosidases and glycosyltransferases that determine the oligosaccharide composition of the cargo, the various sorting signals, GTPases, and adaptor proteins involved, and the tethers and SNAREs that mediate vesicle fusion and deliver the cargo to the next compartment on the pathway. The contributors also examine recent insights into Golgi architecture and positioning, and the way the Golgi fragments and regenerates during cell division.

This volume, which includes discussions of Golgi bypass mechanisms, the evolution and diversity of the Golgi, and the involvement of Golgi in development and human inherited diseases, serves as a comprehensive reference for all cell biologists interested in this intriguing organelle.

Preface

Graham Warren and James Rothman

1. Architecture of the Mammalian Golgi

Judith Klumperman

2. Golgi Glycosylation

Pamela Stanley

3. Golgi Glycosylation and Human Inherited Disease

Hudson H. Freeze and Bobby G. Ng

4. Models for Golgi Traffic: A Critical Assessment

Benjamin S. Glick and Alberto Luini

5. Entry and Exit Mechanisms at the cis-Face of the Golgi Complex

Andrés Lorente-Rodríguez and Charles Barlowe

6. COPI Budding Within the Golgi Stack

Vincent Popoff, Frank Adolf, Britta Brügger, and Felix Wieland

7. The Golgin Coiled-Coil Proteins of the Golgi Apparatus

Sean Munro

8. Organization of SNAREs within the Golgi Stack

Jörg Malsam and Thomas H. Söllner

9. Mechanisms of Protein Retention in the Golgi

David K. Banfield

10. Structure of Golgi Transport Proteins

Daniel Kümmel and Karin M. Reinisch

11. Golgi and Related Vesicle Proteomics: Simplify to Identify

Joan Gannon, John J.M. Bergeron, and Tommy Nilsson

12. Entry at the Trans Face of the Golgi

Suzanne R. Pfeffer

13. PKD Regulates Membrane Fission to Generate TGN to Cell Surface Transport Carriers

Vivek Malhotra and Felix Campelo

14. Signaling at the Golgi

Peter Mayinger

15. Golgi Positioning

Smita Yadav and Adam D. Linstedt

16. Golgi During Development

Weimin Zhong

17. Golgi Biogenesis

Yanzhuang Wang and Joachim Seemann

18. Golgi Bypass: Skirting Around the Heart of Classical Secretion

Adam G. Grieve and Catherine Rabouille

19. Evolution and Diversity of the Golgi Apparatus

Mary J. Klute, Paul Melançon, and Joel B. Dacks

20. Evolutionary Forces Shaping the Golgi Glycosylation Machinery: Why Cell Surface Glycans are Universal to Living Cells

Ajit Varki

Index