Embryonic stem (ES) cells are important vehicles for transfer of genes into mice. When combined with normal pre-implantation embryos and transferred to foster mothers, ES cells contribute to the development of many different tissues, including the germline. When these cells are genetically manipulated, this approach discloses much about the function of specific genes.
Detailed methods for using ES cells are described in the manual Manipulating the Mouse Embryo: A Laboratory Manual, Second Edition.
This video guide complements Manipulating the Mouse Embryo: A Laboratory Manual, Second Edition and Transgenic Techniques in Mice: A Video Guide. It provides step-by-step demonstrations of the following procedures required for making mutant mice.