© 1988 • 726 pp., illus., appendices, bibliography, index
Paper • $87.00 • ISBN  978-087969314-5

Since its publication in 1988, Antibodies: A Laboratory Manual, by Harlow and Lane, has become a classic, an essential resource for molecular biology, immunology, and cell culture labs. In order to keep the book in print, Cold Spring Harbor Laboratory Press eventually produced the paperback edition currently available for sale.

Now, after 25 years, a second edition is being published in September 2013. Revised, extended and updated by Edward Greenfield of the Dana-Farber Cancer Center, the material has been recast with extensive new information and new chapters have been added. The new edition provides clear, authoritative, current and up-to-date protocols with background information and troubleshooting advice. The book is an invaluable resource for all those engaged in antibody research and development. If you wish to place a pre-publication order at 15% discount so that the new edition ships to you immediately upon publication, please click here.

  “[Antibodies aims] to put current immunological methodology into the hands of non-specialists. Happily, Harlow and Lane have succeeded admirably and this manual seems certain also to gain a place on the laboratory bench (too useful to remain on the bookshelf) of a wide variety of biologists—even immunologists. The volume opens with four introductory chapters summarizing key features of the immune response; these provide a succinct overview of the field and are very well written. The scope of the rest of the book includes techniques of immunization and bleeding, monoclonal antibodies and hybridomas, protein labelling, immunoassays (mostly those using solid supports), immunoprecipitation of cellular antigens, immunoblotting and immunoaffinity purification. The methods are presented as easy-to-follow ‘1-2-3 protocols,’ and assume relatively little experience. Rationales for procedures are often presented, which helps to remove some of the mystique that has been associated with immunological methodology.”
      —Nature

Preface

1. Immune Response

2. Antibody Molecules

3. Antibody-Antigen Interactions

4. Antibody Response

5. Immunizations

6. Monoclonal Antibodies

7. Growing Hybridomas

8. Storing and Purifying Antibodies

9. Labeling Antibodies

10. Cell Staining

11. Immunoprecipitation

12. Immunoblotting

13. Immunoaffinity Purification

14. Immunoassay

15. Reagents

Appendices

References

Index