© 2003 • 764 pp., illus., appendices, index
Paperback • $169.00 • ISBN  978-087969591-0

Regarded as the "Bible" of mammalian embryo manipulation techniques since the 1986 publication of the first edition of Manipulating the Mouse Embryo: A Laboratory Manual, the third edition of this essential laboratory resource has now been completely reorganized, rewritten, and updated by a new cast of authors. The result is a compilation of new, cutting–edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques. The "gold standard" techniques for applying recombinant DNA technology to investigations of mammalian embryonic development included in the first two editions of this book are also updated and recast, as is the summary of the current state of understanding of mouse development at the molecular level. This book is the premier authoritative and comprehensive source of technical and theoretical guidance for mouse developmental biologists and geneticists and is an essential resource for newcomers to these fields.

• New authors are leading authorities in mouse development and genetics, as were authors of prior editions.
• All new illustration and new design: All illustrations have been redrawn; extensive use of new illustrations and photographs of techniques and results; a new, contemporary design presents techniques more clearly.
• New explanatory text: More background information for techniques is presented as well as more extensive troubleshooting information.
• New, functional organization and techniques.

  “This manual will become rapidly a reference and find its place in most laboratories. It is usually difficult to reach broad consensus within the scientific field. However, I am sure that the current edition of this classical manual will delightfully and equally satisfy research scientists and specialized technicians within the field of mouse embriology and transgenesis. Undoubtedly, at present, this manual should be the first recommendation to any person willing to work with transgenic or knockout mice.”
      —Transgenic Research

  “This manual is a welcome update, which grew out of teaching material for two practical courses in the Molecular Embryology of the Mouse held at Cold Spring Harbor Laboratory in 1983 and 1984. The previous editions of what is affectionately known as the “Mouse Manual” became and remain the definitive reference sources on this subject. The current authors have taken the mantle of authorship from Brigid Hogan, Rosa Beddington, Frank Costantini, and Elizabeth Lacy and have extensively reorganized and rewritten the new edition. It contains 267 more pages than the second edition, which is largely reflective of the significant advances that have occurred since 1994. This is not just a “how–to” manual for those who seek to manipulate the mouse embryo and genome, but a useful, concise reference on mouse laboratory strain origins, genetics, and embryology and their places in contemporary biology and medicine.”
      —The Quarterly Review of Biology

Chapter 1. Developmental Genetics and Embryology of the Mouse: Past, Present, and Future

Chapter 2. Summary of Mouse Development

Chapter 3. Production of Transgenic and Chimeric Mice: General Issues

Chapter 4. Recovery and In Vitro Culture of Pre-implantation–Stage Embryos

Chapter 5. Isolation, Culture, and Manipulation of Post-implantation Embryos

Chapter 6. Surgical Procedures

Chapter 7. Production of Transgenic Mice

Chapter 8. Isolation, Culture, and Manipulation of Blastocyst–derived Stem Cells

Chapter 9. Vector Designs for Embryonic Stem Cell-based Transgenesis and Genome Alterations

Chapter 10. Introduction of Foreign DNA into Embryonic Stem Cells

Chapter 11. Production of Chimeras

Chapter 12. Detection and Analysis of Mouse Genome Alterations and Specific Sequences

Chapter 13. Parthenogenesis, Pronuclear Transfer, and Mouse Cloning

Chapter 14. Assisted Reproduction: Ovary Transplantation, In Vitro Fertilization, Artificial Insemination, and Intracytoplasmic Sperm Injection

Chapter 15. Cryopreservation, Rederivation, and Transport of Mice

Chapter 16. Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems

Chapter 17. Setting up and Equipping a Micromanipulation Lab

Appendices