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Lab Ref, Volume 2
A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench


Subject Area(s):  Microscopy and ImagingMolecular BiologyProteins and ProteomicsLaboratory Manuals/HandbooksPlant Biology

Edited by Albert S. Mellick, Cold Spring Harbor Laboratory, New York; Linda Rodgers, Cold Spring Harbor Laboratory, New York

View two different sets of sample pages from the book here and here

   

© 2007 • 248 pp., illus., appendix, index
Concealed wire binding • $40.00 • ISBN  978-087969815-7


 

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Lab Ref, Volume 1, A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench
$25

Description

Like its predecessor Lab Ref, Volume 1, this book is a handy benchtop source of recipes and information needed for common laboratory protocols. Lab Ref, Volume 2 contains recipes for procedures as diverse as RNA interference, imaging, proteomics, and quantitative nucleic acid analysis, and is organized by application for quick and easy reference. All recipes were derived from recent manuals published by Cold Spring Harbor Laboratory Press, and each recipe is cross-referenced to its manual of origin. Reference tables and charts are included. To meet the growing need for computational resources in the biology laboratory, Lab Ref, Volume 2 also has an expanded Web site section with sources for biological materials, databases for acquiring and annotating genomic and proteomic data, and links to downloadable software.

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Contents

Introduction
SECTION 1: QUANTITATIVE NUCLEIC ACID ANALYSIS
Isolation of Total DNA
Isolation of Total RNA
Selection of Poly(A) RNA from Total
Isolation and Amplification of RNA from Single Cells and Small Amounts of Tissue
Hybridization Analysis
Preparation of Nucleic Acid (Target) for Array Hybridization Analysis
Preparation of Nucleic Acid (Probe) for Array Hybridization Analysis
Other Quantitative Methods
SECTION 2: RNAi
RNAi in C. elegans
RNAi in D. melanogaster
RNAi in Plants
RNAi in Mammalians
RNAi in Mouse Oocytes and Early Embryos
SECTION 3: IMAGING
Preparation of Embryos for Live Cell Imaging
Imaging Fixed Tissue
Preparation of Mice for Imaging
Intracellular Imaging
General Information for Imaging
SECTION 4: PROTEIN ANALYSIS/PROTEOMICS
Basic Methods in Protein Analysis for Proteomics
Extraction of Proteins
Advanced Protocols in Identifying Protein–Protein Interactions for Proteomics
SECTION 5: ARABIDOPSIS
Microscopy
Genetic Manipulation
Protein Extraction
Nuclear Extraction
Histochemistry of GUS Fusion
SECTION 6: MURINE
In Vitro Culturing of Embryonic Stem Cells
Genotyping
Immunohistochemistry of Whole-mount Embryos
Other Buffers and Solutions
SECTION 7: STORAGE AND SHIPMENT OF BIOLOGICAL SAMPLES
SECTION 8: USEFUL FACTS AND TABLES
A. Useful Information and Reference Tables
B. Nomenclature Guide
C. Useful World Wide Web Sites
Appendix
Index
 
 

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