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Basic Methods in Microscopy
Protocols and Concepts from Cells: A Laboratory Manual

By David L. Spector, Cold Spring Harbor Laboratory; Robert D. Goldman, Northwestern University Medical School, Chicago

© 2006 • 382 pp., illus., index
Paperback • $97.00 • ISBN 978-087969751-8

Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manual Cells: A Laboratory Manual, provide a solid course in the basics of using the microscope in a biology laboratory.

Basic Methods in Microscopy provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events.

This manual is an important tool for any biology researcher employing imaging as a research method.

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Molecular Approaches to Controlling Cancer
(Cold Spring Harbor Symposia on Quantitative Biology LXX)

Edited By Bruce Stillman, Cold Spring Harbor Laboratory; David Stewart, Cold Spring Harbor Laboratory

© 2005 • 538 pp., illus., index
(Paperback edition does not include online access)
Paperback • $126 $25.20 • ISBN 978-087969774-7

The 2005 Cold Spring Harbor Symposium on Quantitative Biology focused on molecular strategies aimed at understanding and controlling the cancerous state. The resulting volume, with contributions from the world’s leading laboratories, covers many aspects of cancer genetics, including DNA repair, telomeres, animal models, stem cells, and therapeutic approaches.

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Molecular Approaches to Controlling Cancer
(Cold Spring Harbor Symposia on Quantitative Biology LXX)

Edited By Bruce Stillman, Cold Spring Harbor Laboratory; David Stewart, Cold Spring Harbor Laboratory

© 2005 • 538 pp., illus., index
(Hardcover edition includes online access; call for information and registration)
Hardcover • $310 $62.00 • ISBN 978-087969773-0

The 2005 Cold Spring Harbor Symposium on Quantitative Biology focused on molecular strategies aimed at understanding and controlling the cancerous state. The resulting volume, with contributions from the world’s leading laboratories, covers many aspects of cancer genetics, including DNA repair, telomeres, animal models, stem cells, and therapeutic approaches.

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Genome Research Special Issue on Human Genome Variation

Edited By Hillary Sussman, Cold Spring Harbor Laboratory Press; Aravinda Chakravarti, Case Western Reserve University; Richard Gibbs, Baylor College of Medicine; Eric Green, National Human Genome Research Institute; Richard Myers, Stanford University School of Medicine; William Pavan, National Human Genome Research Institute; Evan Eichler, University of Washington

© 2005 • ISSN: 1088-9051; Volume 15, Genome Research Special Issue on Human Genome Variation
Journal • $25 • ISBN 1088-9051 V15-N11

The Genome Research November issue is a special issue devoted to a subjectof great current interest, Human Genome Variation. The human genome consistsof 3 billion base pairs of DNA, the sequence of which is 99.9% identicalbetween any two unrelated people. The sites in the remaining 0.1%, wherethe DNA sequence commonly varies among people, are important for understandingsusceptibility to complex diseases such as diabetes, cancer, stroke, heartdisease, and psychiatric disorders.  This special issue is publishedconcordant with Nature’s publication of The International HapMap Consortium’shaplotype map of the human genome, which is a public resource describingcommon genetic variation in the human genome expected to guidethe design of studies aimed at finding variants contributing to human disease.

This issue includes reports on diverse topics relating to populationstructure, demography, evolution, and whole genome association testing,including large–scale recombination patterns and linkage disequilibriumamong populations, ascertainment bias in human genome–wide studies, novelmethodologies to identify single nucleotide polymorphisms (SNPs)associated with cis–acting regulatory variants, choosing tagging SNPs, using SNPs toidentify regions of positive selection in human populations, the role ofsegmental duplications in genomic disorders and specific examples of howthese studies yield biologically relevant results in the form ofidentified susceptibility loci for important human diseases.  This special issue willalso include a perspective on the determinants of  success of whole genomeassociation testing, A User’s Guide to the HapMap Project website, and aposter that has been designed to be an educational resource demonstratingthe data used to describe the patterns of human variation.

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Protein-Protein Interactions: A Molecular Cloning Manual, Second Edition

Edited By Erica A. Golemis, Fox Chase Cancer Center, Philadelphia; Peter D. Adams, Fox Chase Cancer Center, Philadelphia

© 2005 • 938 pp., illus., index
Paperback • $173 $34.60 • ISBN 978-087969723-5

This is an updated edition of a manual that provides a thorough collection of the technical and theoretical issues involved in the study of protein associations, including standard methods, biophysical approaches, and, in a final section, a collection of computational methods for integrating and analyzing interactions.

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Protein-Protein Interactions: A Molecular Cloning Manual, Second Edition

Edited By Erica A. Golemis, Fox Chase Cancer Center, Philadelphia; Peter D. Adams, Fox Chase Cancer Center, Philadelphia

© 2005 • 938 pp., illus., index
Hardcover • $231 $46.20 • ISBN 978-087969722-8

This is an updated edition of a manual that provides a thorough collection of the technical and theoretical issues involved in the study of protein associations, including standard methods, biophysical approaches, and, in a final section, a collection of computational methods for integrating and analyzing interactions.

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Laboratory Research Notebook

© 2005 • 50 pp.
Paperback • $12.00 $2.40 • ISBN 978-087969760-0

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Laboratory Research Notebook

© 2005 • 100 pp.
Hardcover • $9.00 • ISBN 978-087969759-4

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The Inside Story: DNA to RNA to Protein

Edited By Jan Witkowski, Cold Spring Harbor Laboratory

© 2005 • 382 pp., illus., index
Paperback • $29 $14.50 • ISBN 978-087969750-1

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Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual, 2005 Edition

By David C. Amberg, Upstate Medical University, Syracuse; Daniel J. Burke, University of Virginia Medical Center, Charlottesville; Jeffrey N. Strathern, National Cancer Institute

© 2005 • 230 pp., illus., index
Paperback • $96.00 • ISBN 978-087969728-0

“Methods in Yeast Genetics” is a course that has been offered annually at Cold Spring Harbor Laboratory for the last thirty years. This is an updated edition of the course manual, which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology. Since the last edition of the manual was published (2000), revolutionary advances in genomics and proteomics technologies have had a significant impact on the field. This updated edition reflects these advances, and also includes new techniques involving vital staining, visualization of Green Fluorescent Protein, new drug resistance markers, high-copy suppression, Tandem Affinity Protein tag protein purification, gene disruption by double-fusion polymerase chain reaction, and many other recent developments.