© 2003 • 926 pp., illus., appendices, index
Paperback • $184 • ISBN  978-087969554-5

This title also available in: Hardcover

Completion of sequencing of the human genome, as well as those of many other organisms, has now opened the door for exploration of the proteome, the many thousands of interacting proteins in a given organism. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathways and for performing functional assays. Proteins and Proteomics: A Laboratory Manual, authored by Richard Simpson of the Ludwig Institute for Cancer Research, Melbourne, provides the first authoritative and wide–ranging protocol-based approach to proteomics. Presented as a logical strategy for analyzing proteomes, Proteins and Proteomics provides information about protein structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive background information and references are provided regarding the theoretic aspects of the techniques presented as well as their applications. This manual is an essential laboratory tool for genetics and molecular biology investigators moving from studies of genomics and genotype to those of proteomics and phenotype. It is invaluable for cell biologists, developmental biologists, and neuroscientists exploring the intricacies of the proteome. It will also be an excellent resource for protein chemists and biochemists.

  "Proteins and Proteomics: A Laboratory Manual is an invaluable information tool both for the experienced protein chemist who bravely ventures into the new world of proteomics and for the novice to proteins and proteomes. By focusing on what is currently considered the bedrock of proteomics technologies, Professor Simpson ensures that—in spite of the rapid advances that characterize contemporary proteomics research—this volume will remain relevant and current for years to come."
      —From the Preface by Ruedi Aebersold

“. . . the proteome is multidimensional: a single gene can specify a number of different protein products that may themselves be modified post–translationally by the covalent attachment of a range of functional groups. Moreover, proteins commonly occur in complexes whose composition and conformation have a profound effect on both their activity and their stability. Proteomics attempts to grapple with this complexity by using a vast range of molecular, chemical and genetic techniques.

This superb volume embraces this diversity and, even if proteomic techniques are far from comprehensive in their scope, this weighty tome is certainly comprehensive in its description of them. Everything is here, from detailed laboratory protocols to clear explanations of the theoretical basis of the methods used. The book is as up–to–date as possible. It is peppered with references from 2002, and deals fully with recent technical advances, such as protein microarrays.

Chapter 1. Introduction to Proteomics

Chapter 2. One–dimensional Polyacrylamide Gel Electrophoresis

Chapter 3. Preparing Cellular and Subcellular Extracts

Chapter 4. Preparative Two–dimensional Gel Electrophoresis with Immobilized pH Gradients

Chapter 5. Reversed–phase High–performance Liquid Chromatography

Chapter 6. Amino– and Carboxy– terminal Sequence Analysis

Chapter 7. Peptide Mapping and Sequence Analysis of Gel–resolved Proteins

Chapter 8. The Use of Mass Spectrometry in Proteomics

Chapter 9. Proteomic Methods for Phosphorylation Site Mapping

Chapter 10. Characterization of Protein Complexes

Chapter 11. Making Sense of Proteomics: Using Bioinformatics to Discover a Protein?s Structure, Functions, and Interactions