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Basic Methods in Protein Purification and Analysis: A Laboratory Manual

Edited By Richard J. Simpson, Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Peter D. Adams, Fox Chase Cancer Center, Philadelphia; Erica A. Golemis, Fox Chase Cancer Center, Philadelphia

© 2009 • 436 pp., illus., appendices, index
Paperback • $97 • ISBN 978-087969-867-6

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein–protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods.

The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein–Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts—a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein–protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases.

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Basic Methods in Protein Purification and Analysis: A Laboratory Manual

Edited By Richard J. Simpson, Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Peter D. Adams, Fox Chase Cancer Center, Philadelphia; Erica A. Golemis, Fox Chase Cancer Center, Philadelphia

© 2009 • 436 pp., illus., appendices, index
Hardcover • $162.00 • ISBN 978-087969868-3

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein–protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods.

The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein–Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts—a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein–protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases.

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Cold Spring Harbor Monograph Archive

© 2009
Online • $3000 • ISBN 978-087969-941-3

The Cold Spring Harbor Monograph Archive is the complete collection of scholarly monographs published by Cold Spring Harbor Laboratory Press from 1970 to 2009. The archive's 59 full-text volumes provide the life science community with definitive reviews of progress in areas of molecular, cell, and developmental biology, genetics, evolutionary biology, neuroscience, cancer biology, and molecular pathology. Each text is written and commissioned by foremost researchers in their particular discipline.The archive is an unmatched resource for its breadth of coverage in key topics and provides an in-depth account of developments as they occurred in numerous fields

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The Strongest Boy in the World: How Genetic Information is Reshaping Our Lives, Updated and Expanded Edition

By Philip R. Reilly

© 2008 • 300 pp., illus., bibliography, index
Paperback • $16 • ISBN 978-087969943-7

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Control and Regulation of Stem Cells
(Cold Spring Harbor Symposia on Quantitative Biology LXXIIl)

Edited By Bruce Stillman, Cold Spring Harbor Laboratory; David Stewart, Cold Spring Harbor Laboratory; Terri Grodzicker, Cold Spring Harbor Laboratory

© 2008 • 614 pp., illus., indexes
(Paperback edition does not include online access)
Paperback • $126 $63.00 • ISBN 978-087969862-1

Based on presentations by world-renowned investigators at the 73rd annual Cold Spring Harbor Symposium on Quantitative Biology, this volume reviews the latest advances in research on the control and regulation of stem cells. The topics covered include nuclear reprogramming, regulation of stem cell self-renewal and differentiation, the stem cell niche, and signaling and gene regulation in stem cells. Studies of embryonic stem cells and adult stem cells are covered, along with research shedding light on the roles of these cells in regeneration and cancer. 

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Control and Regulation of Stem Cells
(Cold Spring Harbor Symposia on Quantitative Biology LXXIIl)

Edited By Bruce Stillman, Cold Spring Harbor Laboratory; David Stewart, Cold Spring Harbor Laboratory; Terri Grodzicker, Cold Spring Harbor Laboratory

© 2008 • 614 pp., illus., indexes
(Hardcover edition includes online access; call for information and registration)
Hardcover • $310 $155.00 • ISBN 978-087969861-4

Based on presentations by world-renowned investigators at the 73rd annual Cold Spring Harbor Symposium on Quantitative Biology, this volume reviews the latest advances in research on the control and regulation of stem cells. The topics covered include nuclear reprogramming, regulation of stem cell self-renewal and differentiation, the stem cell niche, and signaling and gene regulation in stem cells. Studies of embryonic stem cells and adult stem cells are covered, along with research shedding light on the roles of these cells in regeneration and cancer. 

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Grounds for Knowledge
A Guide to Cold Spring Harbor Laboratory’s Landscapes and Buildings
Introducing the Bungtown Botanical Garden

By Elizabeth L. Watson; Landscape Photography by Peter Stahl

© 2008 • 208 pp., illus., maps, appendices, bibliography, index
Hardcover • $30.00 • ISBN 978-087969799-0

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Neither Gods Nor Beasts
How Science Is Changing Who We Think We Are

By Elof A. Carlson, Emeritus, State University of New York at Stony Brook

© 2008 • 180 pp., index
Hardcover • $29 $14.50 • ISBN 978-087969786-0

Traditional views of human nature focus on the supernatural, defining us as creatures with souls, minds, and spirits that transcend our physical attributes. In this provocative book, distinguished scientist and historian Elof Axel Carlson argues for a different understanding of ourselves based on our biology—cellular organization, genetics, life cycle, evolution, and our origins as a species. This interpretation does not negate our capacity for imagination, spiritual and emotional yearnings, or aesthetic appreciation for art, music, and literature. Carlson challenges educators, the media, and public policy makers to integrate the evidence from science more fully into our understanding of ourselves.

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A Short Guide to the Human Genome

By Stewart Scherer

© 2008 • 173 pp., illus., index
Paperback • $29 $20.30 • ISBN 978-087969791-4

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Life Illuminated: Selected Papers from Cold Spring Harbor
Volume 2, 1972–1994

Edited By Jan A. Witkowski, Cold Spring Harbor Laboratory, New York; Alexander Gann, Cold Spring Harbor Laboratory, New York; Joseph F. Sambrook, Peter MacCallum Cancer Institute, East Melbourne, Australia

© 2008 • 242 pp., illustrations from original articles, indexes, CD
Hardcover • $39 $19.50 • ISBN 978-087969804-1

This book is the second volume of an intellectual history of the science done at CSHL (the first volume, Illuminating Life, showed that genetics became the dominant theme of research at CSH by as early as 1904). The appointment of James Watson as Director of the Laboratory in 1968 set off the explosive research development at CSH, as he recruited widely and wisely teams of investigators with diverse scientific interests. From this collection of papers, presented in full on the accompanying CD, several themes emerge: the characterization and exploitation of mobile genetic elements; the mechanics of DNA replication and regulation of the cell cycle; the behavior and internal architecture of cells; how viruses induce tumors; the discovery of cancer genes; the characteristics of neurons; and the invention of techniques that make possible further progress. Each theme is introduced in the context of the science of the time, and each paper has a commentary by, in most cases, one of its authors. Life Illuminated is a story of scientific innovation and achievement, told in the words of the investigators themselves.