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Next-Generation DNA Sequencing Informatics

Edited By Stuart M. Brown, New York University School of Medicine

© 2013 • 241 pp., illus. (48 4C & 15 B&W), index
Hardcover • $59 • ISBN 978-1-936113-87-3

Next-generation DNA sequencing (NGS) technology has revolutionized biomedical research, making complete genome sequencing an affordable and frequently used tool for a wide variety of research applications. Bioinformatics methods to support DNA sequencing have become a critical bottleneck for many researchers and organizations wishing to make use of NGS technology. This book provides a thorough introduction to the necessary informatics methods and tools for operating NGS instruments and analyzing NGS data. The book also provides extensive reference to best-practice bioinformatic methods for the most commonly used NGS technologies and applications. The book also includes reference to, and guidance on, the setup and use of essential software for NGS data analysis. This is the first book of its kind to address the informatics needs of scientists who wish to take advantage of the explosion of research opportunities offered by new DNA sequencing technologies.

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NF-κB: A Network Hub Controlling Immunity, Inflammation, and Cancer

Edited By Michael Karin, University of California, San Diego; Louis M. Staudt, National Cancer Institute

© 2010 • 272 pp., illus. (42 color, 10 b/w), index
Trim size: 7” x 10”
Paperback • $49 • ISBN 978-1-936113-55-2

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Nuclear Organization and Function
(Cold Spring Harbor Symposia on Quantitative Biology LXXV)

Edited By Terri Grodzicker, Cold Spring Harbor Laboratory; David Spector, Cold Spring Harbor Laboratory; David Stewart, Cold Spring Harbor Laboratory; Bruce Stillman, Cold Spring Harbor Laboratory

© 2010 • 630 pp., illus. (202 color, 74 b&w), indexes
(Hardcover edition includes online access; call for information and registration)
Hardcover • $318.00 • ISBN 978-1-936113-07-1

This volume is based on presentations by the world-renowned investigators who gathered at the 75th annual Cold Spring Harbor Symposium on Quantitative Biology to discuss the organization and function of the cell nucleus. It reviews the latest advances in research into nuclear structure, the organization of the genome within the nucleus, and spatiotemporal coordination of nuclear processes. The topics examined include nuclear domains, chromatin organization, transcription and RNA processing, DNA replication, nuclear reprogramming, and epigenetics. Cancer, premature aging syndromes, and other diseases that may be associated with altered nuclear organization are also covered.

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Nuclear Organization and Function
(Cold Spring Harbor Symposia on Quantitative Biology LXXV)

Edited By Terri Grodzicker, Cold Spring Harbor Laboratory; David Spector, Cold Spring Harbor Laboratory; David Stewart, Cold Spring Harbor Laboratory; Bruce Stillman, Cold Spring Harbor Laboratory

© 2010 • 630 pp., illus. (202 color, 74 b&w)
(Paperback edition does not include online access)
Paperback • $129.00 • ISBN 978-1-936113-08-8

This volume is based on presentations by the world-renowned investigators who gathered at the 75th annual Cold Spring Harbor Symposium on Quantitative Biology to discuss the organization and function of the cell nucleus. It reviews the latest advances in research into nuclear structure, the organization of the genome within the nucleus, and spatiotemporal coordination of nuclear processes. The topics examined include nuclear domains, chromatin organization, transcription and RNA processing, DNA replication, nuclear reprogramming, and epigenetics. Cancer, premature aging syndromes, and other diseases that may be associated with altered nuclear organization are also covered.

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The Nucleus

Edited By Tom Misteli, National Cancer Institute/National Institutes of Health; David L. Spector, Cold Spring Harbor Laboratory

© 2011 • 517 pp., illus. (82 color, 17 b/w), index
Trim size: 7” x 10”
Hardcover • $135 $33.75 • ISBN 978-0-879698-94-2

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The Origins of Life

Edited By David Deamer, University of California, Santa Cruz; Jack W. Szostak, Howard Hughes Medical Institute, Massachusetts General Hospital

© 2010 • 318 pp., illus. (47 color, 50 b/w), index
Trim size: 7” x 10”
Hardcover • $135 $33.75 • ISBN 978-1-936113-04-0

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The p53 Family

Edited By Arnold J. Levine, Institute for Advanced Study, Princeton; David P. Lane, A *Star (Agency for Science, Technology and Research), Singapore

© 2010 • 360 pp., illus. (48 color, 19 b/w), index
Trim size: 7” x 10”
Hardcover • $135 • ISBN 978-0-879698-30-0

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Pandora's Baby: How the First Test Tube Babies Sparked the Reproductive Revolution

By Robin Marantz Henig

© 2006 • 326 pp.
Paperback • $16.95 $8.47 • ISBN 978-087969809-6

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Parkinson's Disease

Edited By Serge Przedborski, Columbia University

© 2012 • 406 pp., illus. (36 4C images and 19 B&W), index
Hardcover • $135 • ISBN 978-1-936113-32-3

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A Passion for DNA: Genes, Genomes, and Society

By James D. Watson, Cold Spring Harbor Laboratory
With an Introduction, Afterword, and Annotations by Walter Gratzer, King’s College London

© 2001 • 266 pp., index
(Published /distributed in conjunction with Oxford University Press.
Click More INFO to find special ordering instructions.)
Paperback • $16 • ISBN 978-087969609-2

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A Passion for DNA: Genes, Genomes, and Society

By James D. Watson, Cold Spring Harbor Laboratory
With an Introduction, Afterword, and Annotations by Walter Gratzer, King’s College London

© 2000 • 250 pp., index
(Published /distributed in conjunction with Oxford University Press.
Click More INFO to find special ordering instructions.)
Cloth • $26 • ISBN 978-087969581-1

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Pattern Formation during Development
(Cold Spring Harbor Symposia on Quantitative Biology LXII)

© 1997 • 570 pp., illus., color plates, appendices, indexes
Cloth • $131 $26.20 • ISBN 978-087969535-4

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Phage and the Origins of Molecular Biology, The Centennial Edition

Edited By John Cairns, Cold Spring Harbor Laboratory; Gunther S. Stent, University of California, Berkeley; James D. Watson, Harvard University

© 2007 • 394 pp., illus., timeline, photo gallery
Hardcover • $30.00 • ISBN 978-087969800-3

This hugely influential book, published in 1966 as a 60th birthday tribute to Max Delbrück, is now republished as The Centennial Edition. On first publication, the book was hailed as “[introducing] into the literature of science, for the first time, a self–conscious historical element in which the participants in scientific discovery engage in writing their own chronicle. As such, it is an important document in the history of biology...” (Journal of History of Biology). And in another review it was described as “required reading for every student of experimental biology...[who] will sense the smell and rattle of the laboratory” (Bioscience). The book was a formative influence on many of today’s leading scientists.

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Phage Display: A Laboratory Manual

By Carlos F. Barbas III, The Scripps Research Institute; Dennis R. Burton, The Scripps Research Institute; Jamie K. Scott, Simon Fraser University; Gregg J. Silverman, University of California, San Diego

© 2001 • 736 pp., illus., index
This book has been produced using print on demand technology.
Paperback • $81.00 • ISBN 978-087969740-2

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Prion Biology and Diseases, Second Edition
(Cold Spring Harbor Monograph Series 41)

Edited By Stanley B. Prusiner, University of California, San Francisco

© 2004 • 1,050 pp., illus., color plates, glossary, appendix, index
Hardcover • $147 $29.40 • ISBN 978-087969693-1

This volume is a new edition of the most authoritative book on Prion Biology, first published in 1999 and edited by the Nobel Prize–winning founder of the field. This expanded edition has been completely updated, and includes chapters on therapeutics, and diagnostic methods and approaches.

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Protein Homeostasis

Edited By Richard I. Morimoto, Northwestern University; Dennis J. Selkoe, Brigham and Women's Hospital; Jeffrey W. Kelly, Scripps Institute

© 2011 • 349 pp., illus. (17 4C and 7 B&W images), index
Hardcover • $135 • ISBN 978-1-936113-06-4

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Protein Synthesis and Translational Control

Edited By John W.B. Hershey, University of California, Davis; Nahum Sonenberg, McGill University; Michael B. Mathews, New Jersey Medical School, University of Medicine and Dentistry of New Jersey

© 2012 • 352 pp., illustrations, index
Hardcover • $135 • ISBN 978-1-936113-46-0

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Protein-Protein Interactions: A Molecular Cloning Manual, Second Edition

Edited By Erica A. Golemis, Fox Chase Cancer Center, Philadelphia; Peter D. Adams, Fox Chase Cancer Center, Philadelphia

© 2005 • 938 pp., illus., index
Hardcover • $231 $46.20 • ISBN 978-087969722-8

This is an updated edition of a manual that provides a thorough collection of the technical and theoretical issues involved in the study of protein associations, including standard methods, biophysical approaches, and, in a final section, a collection of computational methods for integrating and analyzing interactions.

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Protein-Protein Interactions: A Molecular Cloning Manual, Second Edition

Edited By Erica A. Golemis, Fox Chase Cancer Center, Philadelphia; Peter D. Adams, Fox Chase Cancer Center, Philadelphia

© 2005 • 938 pp., illus., index
Paperback • $173 $34.60 • ISBN 978-087969723-5

This is an updated edition of a manual that provides a thorough collection of the technical and theoretical issues involved in the study of protein associations, including standard methods, biophysical approaches, and, in a final section, a collection of computational methods for integrating and analyzing interactions.

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Proteomics:
A Cold Spring Harbor Laboratory Course Manual

By Andrew J. Link, Vanderbilt University School of Medicine, Nashville, Tennessee; Joshua LaBaer, Harvard University School of Medicine

© 2009 • 228 pp., illus., appendices, index
Hardcover • $154.00 • ISBN 978-087969793-8

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Proteomics:
A Cold Spring Harbor Laboratory Course Manual

By Andrew J. Link, Vanderbilt University School of Medicine, Nashville, Tennessee; Joshua LaBaer, Harvard University School of Medicine

© 2009 • 228 pp., illus., appendices, index
Paperback • $96.00 • ISBN 978-087969787-7

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Regulatory RNAs
(Cold Spring Harbor Symposia on Quantitative Biology LXXI)

Edited By Bruce Stillman, Cold Spring Harbor Laboratory, New York; David Stewart, Cold Spring Harbor Laboratory, New York

© 2006 • 574 pp., illus., indexes
(Hardcover edition includes online access; call for information and registration)
Hardcover • $310 $155.00 • ISBN 978-087969817-1

“Regulatory RNAs” was the theme of the 71st annual Cold Spring Harbor Symposium on Quantitative Biology, where scientists from around the world presented the latest advances in the biology of RNAs, including RNA interference, transcriptional and translational control, RNA editing, and the role of RNAs in biological circuits and epigenetic events. Investigators discussed the latest technologies aimed at large-scale characterization of RNAs, as well as the roles of small RNAs in development and cancer. This volume is a timely survey of this important new area of molecular biology.

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The Ribosome
(Cold Spring Harbor Symposia on Quantitative Biology LXVI)

Edited By Bruce Stillman, Cold Spring Harbor Laboratory

© 2001 • 634 pp., illus., index
Hardcover • $270 $54.00 • ISBN 978-087969619-1

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RNA Worlds: From Life’s Origins to Diversity in Gene Regulation

Edited By John F. Atkins, University of Utah, University College Cork, and Trinity College Dublin; Raymond F. Gesteland, University of Utah; Thomas R. Cech, Howard Hughes Medical Institute, University of Colorado

© 2011 • 366 pp., illus. (73 color, 23 b/w), index
Trim size: 8-1/2” x 10-7/8”
Hardcover • $162.00 • ISBN 978-0-879699-46-8

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RNA: A Laboratory Manual

By Donald C. Rio, University of California, Berkeley; Manuel Ares, Jr., University of California, Santa Cruz; Gregory J. Hannon, Cold Spring Harbor Laboratory; Timothy W. Nilsen, Case Western Reserve University School of Medicine

© 2011 • 586 pp., illus., appendices, index
Hardcover • $246.00 $172.20 • ISBN 978-0-879698-90-4

So much has been learned about RNA in the past 10 years that the ability to purify, analyze, and manipulate RNA molecules is now essential in all kinds of bioscience. Initiating RNA research can be intimidating, but the new book RNA: A Laboratory Manual provides a broad range of up-to-date techniques presented in a functional framework, so that any investigator can confidently handle RNA and carry out meaningful experiments, from the most basic to the highly sophisticated. Originating in four of the field's most prominent laboratories, this manual provides the necessary background and strategies for approaching any RNA investigation, as well as detailed protocols and extensive tips and troubleshooting information. It is required reading for every research laboratory in the life sciences.

About the Authors

Donald C. Rio is Professor of Molecular and Cell Biology at the University of California, Berkeley. His research has focused on nucleic acid biochemistry, nucleic acid–protein interactions, nucleic acid rearrangements, and genome-wide approaches to the role of RNA binding proteins in controlling alternative pre-mRNA splicing. Dr. Rio obtained his Ph.D. with Robert Tjian at the University of California, Berkeley and did postdoctoral work with Gerald Rubin. He was Assistant and Associate Professor at MIT and Associate Member of the Whitehead Institute for Biomedical Research, prior to moving to his current position.

Manuel Ares, Jr. is Professor of Molecular, Cell & Developmental Biology at the University of California, Santa Cruz. He received a Ph.D. with Stephen H. Howell on the cell cycle of Chlamydomonas at UC San Diego (1982) and did postdoctoral work with Alan M. Weiner on snRNA transcription in HeLa cells at Yale. He joined the faculty at UC Santa Cruz in 1987 and has focused on snRNA processing, structure, and function in yeast and mammalian cells. He is currently President of the RNA Society.

Gregory J. Hannon is Professor and Howard Hughes Medical Institute Investigator at Cold Spring Harbor Laboratory. He received his Ph.D. at Case Western Reserve University working with Tim Nilsen (1992) and then moved to Cold Spring Harbor Laboratory for postdoctoral studies with David Beach. A pioneering investigator in the field of RNA interference, his laboratory has characterized many of the major complexes for small interfering RNAs and microRNAs. His lab also investigates the role of small RNAs as oncogenes and tumor suppressors and the use of RNAi libraries to identify therapeutic targets for specific disease subtypes.

Timothy W. Nilsen is Professor and Director of the Center for RNA Molecular Biology at Case Western Reserve University School of Medicine. He is also Professor of Biochemistry and of Medicine. After receiving a Ph.D. from the State University of New York at Albany working with Corrado Baglioni, he joined the faculty at CWRU (1982). His research has focused on RNA biology including the mechanism of action of interferon, the mechanism of SL-addition trans-splicing and mechanisms of regulation of alternative splicing. Currently, his laboratory is investigating the mechanism(s) of microRNA mediated gene regulation in animal cells and the structure and function of messenger ribonucleoprotiens. Dr. Nilsen became Editor in Chief of the journal RNA when it was founded in 1995 and still serves in that capacity. He is on the Editorial Board of Molecular and Cellular Biology and the Board of Reviewing Editors for Science.

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RNA: A Laboratory Manual

By Donald C. Rio, University of California, Berkeley; Manuel Ares, Jr., University of California, Santa Cruz; Gregory J. Hannon, Cold Spring Harbor Laboratory; Timothy W. Nilsen, Case Western Reserve University School of Medicine

© 2011 • 586 pp., illus., appendices, index
Paperback • $169.00 $118.30 • ISBN 978-0-879698-91-1

RNA molecules participate in and regulate a vast array of cellular processes, and the scientific community is now entering a new era in which some aspect of RNA biology—as a tool, a therapeutic, a diagnostic, or part of a fundamental process—is becoming increasingly important. But initiating RNA research can be intimidating, and without a thorough understanding of the challenges and complexities inherent in handling this fragile nucleic acid, forays into the RNA world can be quite frustrating. RNA: A Laboratory Manual provides a broad range of up-to-date techniques so that any investigator can confidently handle RNA and carry out meaningful experiments, from the most basic to the most sophisticated. Originating in four of the field’s most prominent laboratories and written with novices as well as more advanced researchers in mind, this manual provides the necessary background and strategies for approaching any RNA investigation in addition to detailed step-by-step protocols and extensive tips and troubleshooting information. RNA: A Laboratory Manual will enable any researcher to approach a wide variety of RNA-related problems with confidence and a high expectation of success.

About the Authors

Donald C. Rio is Professor of Molecular and Cell Biology at the University of California, Berkeley. His research has focused on nucleic acid biochemistry, nucleic acid–protein interactions, nucleic acid rearrangements, and genome-wide approaches to the role of RNA binding proteins in controlling alternative pre-mRNA splicing. Dr. Rio obtained his Ph.D. with Robert Tjian at the University of California, Berkeley and did postdoctoral work with Gerald Rubin. He was Assistant and Associate Professor at MIT and Associate Member of the Whitehead Institute for Biomedical Research, prior to moving to his current position.

Manuel Ares, Jr. is Professor of Molecular, Cell & Developmental Biology at the University of California, Santa Cruz. He received a Ph.D. with Stephen H. Howell on the cell cycle of Chlamydomonas at UC San Diego (1982) and did postdoctoral work with Alan M. Weiner on snRNA transcription in HeLa cells at Yale. He joined the faculty at UC Santa Cruz in 1987 and has focused on snRNA processing, structure, and function in yeast and mammalian cells. He is currently President of the RNA Society.

Gregory J. Hannon is Professor and Howard Hughes Medical Institute Investigator at Cold Spring Harbor Laboratory. He received his Ph.D. at Case Western Reserve University working with Tim Nilsen (1992) and then moved to Cold Spring Harbor Laboratory for postdoctoral studies with David Beach. A pioneering investigator in the field of RNA interference, his laboratory has characterized many of the major complexes for small interfering RNAs and microRNAs. His lab also investigates the role of small RNAs as oncogenes and tumor suppressors and the use of RNAi libraries to identify therapeutic targets for specific disease subtypes.

Timothy W. Nilsen is Professor and Director of the Center for RNA Molecular Biology at Case Western Reserve University School of Medicine. He is also Professor of Biochemistry and of Medicine. After receiving a Ph.D. from the State University of New York at Albany working with Corrado Baglioni, he joined the faculty at CWRU (1982). His research has focused on RNA biology including the mechanism of action of interferon, the mechanism of SL-addition trans-splicing and mechanisms of regulation of alternative splicing. Currently, his laboratory is investigating the mechanism(s) of microRNA mediated gene regulation in animal cells and the structure and function of messenger ribonucleoprotiens. Dr. Nilsen became Editor in Chief of the journal RNA when it was founded in 1995 and still serves in that capacity. He is on the Editorial Board of Molecular and Cellular Biology and the Board of Reviewing Editors for Science.

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RNA: Life's Indispensable Molecule

By James Darnell, The Rockefeller University

© 2011 • 416 pp., illus., appendix, index
Hardcover • $40.00 • ISBN 978-1-936113-19-4

In RNA: Life’s Indispensable Molecule, Jim Darnell provides a comprehensive and captivating account of RNA research, illuminated by his own life-long and celebrated engagement in the field. Darnell describes how scientists unraveled fundamental questions about the biochemical and genetic importance of RNA—how mRNAs are generated and used to produce proteins, how noncoding and catalytic RNAs mediate key cellular processes, and how RNA molecules likely initiated life on Earth. With a scope extending from the early 20th century to the present day, and with the clarity expected from an accomplished textbook author, he conveys the intellectual context in which these questions first arose and explains how the key experiments were structured and answers obtained. The book is geared towards scientists from the graduate level on up, and will particularly appeal to active investigators in RNA biology, educators of molecular biology and biochemistry, and science historians.

About the Author

James E. Darnell, Jr., M.D. has been Vincent Astor Professor at The Rockefeller University since 1974. His career has included poliovirus research with Harry Eagle at the National Institutes of Health, research with François Jacob at the Pasteur Institute in Paris, and academic appointments at the Massachusetts Institute of Technology, Albert Einstein College of Medicine, and Columbia University. He has mentored over 120 doctoral students and postdoctoral scientists. From the very beginning of his first lab at MIT, Darnell, his students and postdocs have studied RNA, its synthesis, processing, and transcriptional regulation.

Darnell is a member of the National Academy of Sciences and has received numerous awards, including the 2003 National Medal of Science and the 2002 Albert Lasker Award for Special Achievement in Medical Science. He is the coauthor, with S.E. Luria, of General Virology (Wiley) and the founding author with Harvey Lodish and David Baltimore of Molecular Cell Biology

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RNAi: A Guide to Gene Silencing

Edited By Gregory J. Hannon, Cold Spring Harbor Laboratory

© 2003 • 436 pp., illus., index
Printed hardcover • $130 $26.00 • ISBN 978-087969641-2

The ability of double stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way. This volume combines reliable RNAi protocols for a variety of species with discussion of strategies for the effective design of experiments using this important new technique that is changing the way experimental science is done.

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RNAi: A Guide to Gene Silencing

Edited By Gregory J. Hannon, Cold Spring Harbor Laboratory

© 2003 • 436 pp., illus., index
Paperback • $75 $15.00 • ISBN 978-087969704-4

The ability of double stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way. This volume combines reliable RNAi protocols for a variety of species with discussion of strategies for the effective design of experiments using this important new technique that is changing the way experimental science is done.

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A Short Guide to the Human Genome

By Stewart Scherer

© 2008 • 173 pp., illus., index
Paperback • $29 $20.30 • ISBN 978-087969791-4

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Signaling & Gene Expression in the Immune System
(Cold Spring Harbor Symposia on Quantitative Biology LXIV)

© 1999 • 621 pp., illus., color plates, appendices, index
Paper • $115 $57.50 • ISBN 978-087969570-5

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Single-Molecule Techniques: A Laboratory Manual

Edited By Paul R. Selvin, University of Illinois, Urbana-Champaign; Taekjip Ha, University of Illinois, Urbana-Champaign

© 2008 • 507 pp., illus., appendix, index
Paperback • $169.00 • ISBN 978-087969775-4

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The Skeletal System
(Cold Spring Harbor Monograph Series 53)

Edited By Olivier Pourquié, Stowers Institute for Medical Research, Kansas City, Missouri

© 2009 • 365 pp., illus., index
Hardcover • $95 $47.50 • ISBN 978-087969825-6

The thirteen chapters presented in this book summarize our current understanding of the development, biology, and evolution of the vertebrate skeleton. Written by experts in the field, chapters cover everything from the differentiation of chondrocytes, limb and craniofacial patterning, and the origins and evolution of bone to the genetics of human skeletal disease. It is a useful reference for scientists and clinicians wishing to learn how the skeleton is built and works.

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Speaking of Genetics: A Collection of Interviews

Edited By Jane Gitschier, Institute for Human Genetics, University of California, San Francisco

© 2011 • 259 pp., illus., further reading, index
Paperback • $19.00 • ISBN 978-1-936113-03-3

These 22 interviews were conducted by University of California Professor Jane Gitschier for publication in the scientific journal PLoS Genetics over the past 5 years. Here these interviews are presented as a single, stand-alone collection for the first time.

The collection makes a compelling read by capturing exhilarating moments of discovery on a wide range of genetic questions as recounted by the scientists themselves. We also hear from scientists whose careers have taken on a broader role—a University president, a master teacher, an author, the founder of a company, and an explorer. Further perspective is provided by several non-scientists whose work helps frame our understanding and practice of genetics; these include a judge, a reporter, an administrator, and an historian of science. Together, the interviews in this collection bring to life the practice and societal implications of contemporary genetics.

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Statistics at the Bench: A Step-by-Step Handbook for Biologists

By Martina Bremer, Department of Mathematics, San Jose State University, California; Rebecca W. Doerge, Departments of Statistics and Agronomy, Purdue University, Indiana

© 2010 • 167 pp., illus., indexes
Concealed wire binding • $61.00 $42.70 • ISBN 978-0-879698-57-7

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The Statue Within: An Autobiography

By Francois Jacob
Translated by Franklin Philip

© 1995 • 326 pp., index
This book has been produced using print on demand technology.
Paper • $22 $11.00 • ISBN 978-087969476-0

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Strategies for Protein Purification and Characterization: A Laboratory Course Manual

By Daniel R. Marshak, Cold Spring Harbor Laboratory; Mark W. Knuth, Promega Corporation, Madison, Wisconsin

© 1996 • 396 pp., illus., appendices, index
This book has been produced using print on demand technology.
Spiral binding • $48.00 • ISBN 978-087969385-5

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The Strongest Boy in the World: How Genetic Information is Reshaping Our Lives, Updated and Expanded Edition

By Philip R. Reilly

© 2008 • 300 pp., illus., bibliography, index
Paperback • $16 • ISBN 978-087969943-7

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Sydney Brenner: A Biography

By Errol C. Friedberg, University of Texas Southwestern Medical Center at Dallas

© 2011 • 334 pp., illus. (59 b/w), index
Trim size: 6” x 9”
Hardcover • $40.00 • ISBN 978-0-879699-47-5

Over his long and inspiring career, the Nobel Laureate Sydney Brenner has made some of the most significant and game-changing discoveries in the field of molecular biology. But Brenner’s reach has extended well beyond his own research to inspire new generations of young scientists and to promote the development of science and biotechnology around the world. Based on his personal recollections, with contributions and correspondence from his close friends and colleagues, this book tells the lively story, not only of Brenner himself, but of what came to be known as the “golden age” of biology.About the author: Errol C. Friedberg, M.D., is the Senator Betty and Dr. Andy Andujar Distinguished Professor and Chair at the Department of Pathology, University of Texas Southwestern Medical Center at Dallas. A Fellow of the Royal College of Pathologists and recipient of the Rous Whipple Award from the American Society for Investigative Pathology, Friedberg also serves as Editor-in-Chief of the journal DNA Repair. Friedberg is the author of Cancer Answers, Correcting the Blueprint of Life: An Historical Account of the Discovery of DNA Repair Mechanisms, The Writing Life of James D. Watson, and From Rags to Riches: The Phenomenal Rise of The University of Texas Southwestern Medical Center at Dallas. He is also senior author of the textbook DNA Repair & Mutagenesis, and has edited and annotated a series of interviews published as Sydney Brenner: My Life in Science.

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Symmetry Breaking in Biology

Edited By Rong Li, The Stowers Institute for Medical Research; Bruce Bowerman, Institute of Molecular Biology and Department of Biology, University of Oregon

© 2010 • 301 pp., illus., index
Hardcover • $135 $33.75 • ISBN 978-0-879698-89-8

Symmetry breaking events are critical for the survival of living systems. They are required for cell division, development, and movement in all organisms from single-celled species to human beings. Moreover, in multicellular organisms, symmetry breaking allows the generation of cells with different fates and underpins the complex arrangement of tissues and organs achieved during embryogenesis.

Written and edited by experts in the field, this volume explores how symmetry breaking occurs in biology and the roles of these events at numerous different levels. The contributors examine the mechanisms by which cells polarize, divide asymmetrically, and produce asymmetric structures, providing examples from bacteria, yeast, plants, invertebrates, and mammals.

Including discussions of the molecular basis of polarization mechanisms, asymmetric division of stem cells during development, the generation of left-right asymmetry of the body axis in mammals, and theoretical approaches to symmetry breaking, the volume is a vital reference for molecular, cell, and developmental biologists, as well as physical scientists interested in how and why symmetry breaking occurs in living systems.

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The Synapse

Edited By Morgan Sheng, Genentech, Department of Neuroscience; Bernardo Sabatini, Harvard Medical School; Thomas C. Südhof, Stanford University School of Medicine

© 2012 • 397 pp., illus. (78 4C and 6 B&W images), index
Hardcover • $135 • ISBN 978-1-936113-02-6

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Telomeres, Second Edition
(Cold Spring Harbor Monograph Series 45)

Edited By Titia de Lange, The Rockefeller University; Vicki Lundblad, The Salk Institute for Biological Sciences; Elizabeth Blackburn, University of California, San Francisco

© 2006 • 576 pp., illus., index
Hardcover • $139 $27.80 • ISBN 978-087969734-1

An up–to–date survey of the current exciting state of telomere biology. Telomeres—specialized structures found at the ends of chromosomes— are essential for maintaining the integrity of chromosomes and their faithful duplication during cell division. Chapters in this volume cover telomere structure and function in a range of organisms, focusing on how they are maintained, their roles in cell division and gene expression, and how deficiencies in these structures contribute to cancers and other diseases and even aging.

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Telomeres, Second Edition
(Cold Spring Harbor Monograph Series 45)

Edited By Titia de Lange, The Rockefeller University; Vicki Lundblad, The Salk Institute for Biological Sciences; Elizabeth Blackburn, University of California, San Francisco

© 2006 • 576 pp., illus., index
Paperback • $49 $9.80 • ISBN 978-087969810-2

An up–to–date survey of the current exciting state of telomere biology. Telomeres—specialized structures found at the ends of chromosomes— are essential for maintaining the integrity of chromosomes and their faithful duplication during cell division. Chapters in this volume cover telomere structure and function in a range of organisms, focusing on how they are maintained, their roles in cell division and gene expression, and how deficiencies in these structures contribute to cancers and other diseases and even aging.

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The TGF-β Family
(Cold Spring Harbor Monograph Series 50)

Edited By Rik Derynck, University of California, San Francisco; Kohei Miyazono, University of Tokyo

© 2008 • 1,114 pp., illus., index
Hardcover • $135 $67.50 • ISBN 978-087969752-5

TGF-β is the prototype of a protein superfamily which, in humans, contains at least 35 members, including activins, inhibins, bone morphogenetic proteins, growth/differentiation factors, and Müllerian inhibiting substance. This monograph draws on the world’s leading laboratories to comprehensively cover all aspects of the biology of TGF-β and serve as a reference work for both specialists and researchers less familiar with the field.

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The Cardiovascular System
(Cold Spring Harbor Symposia on Quantitative Biology LXVII)

© 2002 • 566 pp., illus., color plates, appendices, index
Paperback • $115 $46.00 • ISBN 978-087969679-5

Normal cardiovascular function requires the concerted action of many cell types, each capable of adaptive gene expression in response to developmental, physiological, and pathological cues. The genetic basis of cardiovascular function, development, and disease is an area of intense investigation, in the hope of significant insights into the heart and vessels? basic workings and improvements in diagnosis and therapy. This latest volume in a prestigious book series presents a remarkable survey of current progress in these efforts, through the contributions of over fifty of the world's leading investigators. Sections are devoted to angiogenesis, cardiogenesis, homeostasis, development, vascular biology, and cardiovascular repair and therapy. The book is an essential source of ideas, discoveries, and references for clinical scientists and physicians interested in basic cardiac biology, hypertension, atherosclerosis, coronary artery disease, and heart failure.

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The Dog and Its Genome
(Cold Spring Harbor Monograph Series 44)

Edited By Elaine A. Ostrander, National Human Genome Research Institute, NIH; Urs Giger, School of Veterinary Medicine, University of Pennsylvania, Philadelphia; Kerstin Lindblad-Toh, Broad Institute of MIT and Harvard

© 2006 • 584 pp., illus., index
Hardcover • $139 • ISBN 978-087969742-6

Dogs of different breeds can range remarkably in size, shape, and behavior, and yet they all carry essentially the same genome, making them a particularly fascinating model for genome plasticity. The recent release of the complete sequence of the dog genome provides an exciting new context in which to consider such variation. Twenty–five chapters written by experts in the field include various aspects of morphological and behavioral variation in dogs, their origins and domestication, and their unique value as a model system for many common but complex human diseases such as diabetes and cancer.

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The Genome of Homo sapiens
(Cold Spring Harbor Symposia on Quantitative Biology LXVIII)

By Bruce Stillman, Cold Spring Harbor Laboratory; David Stewart, Cold Spring Harbor Laboratory

© 2003 • 544 pp., illus., appendices, index
(Hardcover edition includes online access; call for information and registration)
Hardcover • $310 $62.00 • ISBN 978-087969709-9

The Human Genome Project was completed in 2003, 50 years after the discovery of the structure of DNA and 17 years after an influential debate at the annual Cold Spring Harbor Laboratory Symposium about the Project’s feasibility. The 2003 Symposium was dedicated to examining what has been learned so far from the human genome sequence. This book contains over sixty contributions from the world’s leaders in this field and covers genome structure and evolution, methods of data analysis, lessons from species comparison, and the application of sequence data to the understanding of disease.

Purchasers of the hard cover edition of this book are entitled to access to the Symposia website at www.cshl-symposium.org. The site contains the full text of the written communications from the 2003 Symposium and the Symposia held in 1998 through 2002 (Volumes LXIII–LXVII). Subscribers to the site also gain access to archive photographs and selected papers from the 60–year history of the Annual Symposium.

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The Genome of Homo sapiens
(Cold Spring Harbor Symposia on Quantitative Biology LXVIII)

By Bruce Stillman, Cold Spring Harbor Laboratory; David Stewart, Cold Spring Harbor Laboratory

© 2003 • 544 pp., illus., appendices, index
(Paperback edition does not include online access)
Paperback • $126 $25.20 • ISBN 978-087969710-5

The Human Genome Project was completed in 2003, 50 years after the discovery of the structure of DNA and 17 years after an influential debate at the annual Cold Spring Harbor Laboratory Symposium about the Project’s feasibility. The 2003 Symposium was dedicated to examining what has been learned so far from the human genome sequence. This book contains over sixty contributions from the world’s leaders in this field and covers genome structure and evolution, methods of data analysis, lessons from species comparison, and the application of sequence data to the understanding of disease.

Purchasers of the hard cover edition of this book are entitled to access to the Symposia website at www.cshl-symposium.org. The site contains the full text of the written communications from the 2003 Symposium and the Symposia held in 1998 through 2002 (Volumes LXIII–LXVII). Subscribers to the site also gain access to archive photographs and selected papers from the 60–year history of the Annual Symposium.

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The Molecular Gaze: Art in the Genetic Age

By Suzanne Anker, School of Visual Arts, New York; Dorothy Nelkin, New York University

© 2004 • 216 pp., illus., index
Hardcover • $47 $23.50 • ISBN 978-087969697-9

The gene has become a cultural icon and an increasingly rich source of imagery and ideas for visual artists. Drawing on a wide range of contemporary painting and sculpture, The Molecular Gaze: Art in the Genetic Age explores the moral and bioethical questions these works address. What does it mean to be human? What is “identity” in a society of genetically manipulated individuals? Questions like these are growing louder as genetic technology advances and the public examines the ethical consequences more widely. Suzanne Anker and Dorothy Nelkin, an artist and a social scientist, have written a thought–provoking and visually fascinating book for scientists, artists, students, and general readers intrigued by the anxiety and exhilaration of the genetic age.

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The Unfit: A History of a Bad Idea

By Elof Axel Carlson, Professor Emeritus, State University of New York at Stony Brook

© 2001 • 451 pp., illus., bibliography, indexes
Book of the Year for 2001 in Amazon's Professional Science Category!
Hardcover • $26 $6.50 • ISBN 978-087969587-3

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Times of Triumph, Times of Doubt: Science and the Battle for Public Trust

By Elof Axel Carlson, Professor Emeritus, State University of New York at Stony Brook

© 2006 • 227 pp., index
Hardcover • $39 $15.60 • ISBN 978-087969805-8

The intent and uses of science are a continuing preoccupation, especially in public debates on issues such as new pharmaceuticals, cloning, stem cells, genetically modified foods, and assisted reproduction. Times of Triumph, Times of Doubt, written by the eminent geneticist and historian Elof Carlson, explores the moral foundations of science and their role in these hot–button issues. Carlson chooses a variety of case histories and describes their scientific background and the part played by scientists in the application of their work, including their motivations and reactions to bad outcomes, both real and alleged. He examines why ethical lapses have occurred in these areas, why bad things happen when, for the most part, those who worked on the science had only good intentions in mind, and how such lapses can be prevented from occurring in the future. This exploration of ethics and science is important reading for those interested in issues of science and society, including journalists, theologians, legislators, lawyers, and scientists themselves.

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Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques, Second Edition

By Michael F. Carey, University of California, Los Angeles; Craig L. Peterson, University of Massachusetts Medical School, Worcester; Stephen T. Smale, University of California, Los Angeles

© 2009 • 620 pp., appendix, index
Hardcover • $246.00 • ISBN 978-087969777-8

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Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques, Second Edition

By Michael F. Carey, University of California, Los Angeles; Craig L. Peterson, University of Massachusetts Medical School, Worcester; Stephen T. Smale, University of California, Los Angeles

© 2009 • 620 pp., appendix, index
Paperback • $169.00 • ISBN 978-087969762-4

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Translational Control in Biology and Medicine
(Cold Spring Harbor Monograph Series 48)

Edited By Michael B. Mathews, UMDNJ-New Jersey Medical School, Newark, New Jersey; Nahum Sonenberg, McGill University, Montreal, Canada; John W.B. Hershey, University of California, Davis

© 2007 • 934 pp., illus., index
Hardcover • $139 $34.75 • ISBN 978-087969767-9

The new edition of this successful monograph has been both updated and broadened. Since the previous (second) edition was published in 2000, the structures of the bacterial and eukaryotic ribosomes have been published, advancing our basic understanding of translation and mechanisms involving protein and RNA regulators. In addition, as the title indicates, this edition has a new focus on the role of translational control in human development and disease. This book, with 30 chapters written by experts in the field, is essential reading for anyone interested in the process of translation, its regulation, and how its failure can be the cause of disease.

View two different sets of sample pages from the book here — and here —

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Type 1 Diabetes

Edited By Jeffrey A. Bluestone, University of California at San Francisco; Mark A. Atkinson, University of Florida, College of Medicine; Peter R. Arvan, University of Michigan Health System

© 2012 • 305 pp., illus., index
Hardcover • $135 • ISBN 978-1-936113-21-7

Type 1 diabetes, also known as juvenile-onset or insulin-dependent diabetes mellitus (IDDM), is caused by destruction of insulin-producing β cells in the pancreas by the body’s immune system. It affects hundreds of thousands of individuals worldwide, requiring regular insulin injections to control blood glucose levels and avoid severe consequences of glucose dysregulation. Written by experts in the field, this book provides a detailed examination of our understanding of the disease process and its causes, as well as genetic risk factors, animal models of diabetes, and new treatment strategies that are being developed.

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Untangling the Double Helix
DNA Entanglement and the Action of the DNA Topoisomerases

By James C. Wang, Harvard University

© 2009 • 233 pp., illus., appendices, index
Hardcover • $81.00 • ISBN 978-087969863-8

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Untangling the Double Helix
DNA Entanglement and the Action of the DNA Topoisomerases

By James C. Wang, Harvard University

© 2009 • 233 pp., illus., appendices, index
Paperback • $46.00 • ISBN 978-087969879-9

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We Can Sleep Later: Alfred D. Hershey and the Origins of Molecular Biology

Edited By Franklin W. Stahl, University of Oregon, Eugene

© 2000 • 357 pp., illus., indexes
Hardcover • $41 $20.50 • ISBN 978-087969567-5

An absorbing portrait of the pioneering molecular biologist best known for demonstrating that DNA is the genetic component of phages, through essays and reminiscences from twenty–three distinguished scientists whose work and careers were influenced by the man and his science.

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What a Time I Am Having: Selected Letters of Max Perutz

Edited By Vivien Perutz

© 2009 • 506 pp., illus., index
Hardcover • $40.00 • ISBN 978-087969864-5

Selected by his daughter, Vivien, from Max Perutz’s voluminous correspondence, the letters reproduced here portray their author with a spontaneity and directness no autobiography could have matched. They chronicle Perutz’s adventurous life through his own vivid, erudite and humorous pen, documenting the hopes, roadblocks and moments of elation of his sixty-year quest to understand the molecular biology of hemoglobin. The first great step in this quest — unraveling the molecular structure of hemoglobin — earned Perutz the 1962 Nobel Prize in Chemistry. Narrated against a backdrop of family and friends, politics and war, literature, travels, and Max’s beloved mountains, these letters provide rare insight into the thoughts of a remarkable and very human scientist, and delightful sketches of some of the people he encountered. Starting with lively letters to a girlfriend written in his youth in Vienna and the impressions of a young scientist in Cambridge, the letters progress to the desperate pleas of an “enemy alien” interned in Canada during World War II. The diary of Perutz’s subsequent super-secret war work for the British to build a floating ice airstrip in the North Atlantic, ardent campaigning letters to scientists and politicians, and self-deprecating stories of his own mishaps written to amuse his children and grandchildren are some of the many highlights of these fascinating letters, unique in the annals of recent scientific history. This book is a companion to Georgina Ferry’s Max Perutz and the Secret of Life. Together these volumes provide a portrait of an extraordinary character in the development of molecular biology.

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Wnt Signaling

Edited By Roel Nusse, Stanford University Medical Center; Xi He, Children’s Hospital Boston; Renée van Amerongen, Stanford University Medical Center

© 2013 • 454 pp., illus. (75 4C and 6 B&W), index
Hardcover • $135 • ISBN 978-1-936113-23-1

Wnt proteins are signaling molecules that play critical roles during embryonic development and in the regeneration of adult tissues. They bind to Frizzled and LRP family receptors on the cell surface, triggering a series of events that cause β-catenin to enter the nucleus and activate transcription factors that control cell fate and cell proliferation. Mutations in components of the Wnt pathway lead to developmental defects and are common in cancer.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology covers all aspects of canonical Wnt signaling, as well as β-catenin-independent Wnt signaling and cross-talk with other pathways. The contributors examine the numerous Wnt ligands; their production, secretion, and interactions with components of the extracellular environment; and details of the downstream signaling pathways that mediate the effects of Wnt proteins on cells. The roles of Wnt signaling in stem cell self-renewal, cell polarity, body-axis specification, wound healing, and other aspects of normal development and physiology are also covered.

This volume includes discussion of Wnt signaling in cancer, skeletal defects, neurological disorders, and other human disease states. Thus, it is an indispensable reference for cell and developmental biologists as well as those, especially in the fields of stem cell biology and regenerative medicine, who are interested in targeting the Wnt pathway for therapeutic purposes.

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Won for All: How the Drosophila Genome Was Sequenced

By Michael Ashburner

© 2006 • 107 pp., illus.
Hardcover • $19.95 $9.97 • ISBN 978-087969802-7

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The Writing Life of James D. Watson

By Errol C. Friedberg, University of Texas Southwestern Medical Center

© 2005 • 193 pp., illus., index
Hardcover • $26 $5.20 • ISBN 978-087969700-6

James Watson's fame as a scientist and research leader overshadows his considerable achievements as an innovator in the form and style of scientific communication. This book surveys Watson's books and essays from the perennially best–selling The Double Helix through his classic textbooks of the 1960s and 70s, polemics on ethical questions about genetic technology, to more recent works of autobiography.

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Yeast Intermediary Metabolism

By Dan G. Fraenkel, Harvard Medical School

© 2011 • 434 pp., illus. (138 b/w), appendix, index
Hardcover • $128 • ISBN 978-0-879697-97-6

The intermediary metabolism of small molecules is the meat and potatoes of cell function. The pathways and modes of obtaining energy, degradation and utilization of exogenous organic nutrients, and formation of the building blocks of the main macromolecules were a major focus of research in biology from the turn of the 20th century into the 1970s. Other matters have come to prominence, but the field is active, with interesting problems that are central to biology and medicine. Molecular biology developed through the use of one bacterium, Eschericha coli, with the saying “What’s true for E. coli is true for elephants.” In recent years, an analogous workhorse has been the eukaryotic microbe baker’s yeast, Saccharomyces cerevisiae, used in many studies of cell biology common to multicellular organisms. This book explains metabolism as based on Saccharomyces. The topics include central metabolic pathways; catabolism; fermentation; respiration; biosynthesis of small molecules including cofactors; the metabolism of lipids, polysaccharides, and storage molecules; inorganic ions; transport and compartments; the global analysis of metabolism; and issues of metabolic toxicity. It can be used in courses and as a reference book for research investigators.