© 2003 • 436 pp., illus., index
Printed hardcover • $130 26.00 • ISBN  978-087969641-2
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The ability of double stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way. This volume combines reliable RNAi protocols for a variety of species with discussion of strategies for the effective design of experiments using this important new technique that is changing the way experimental science is done.

  “Because of the overwhelming plethora of studies, this book, written by leaders in the field, arrives as a welcome road map for beginners and experts alike. The volume provides a broad overview of the basic mechanisms and applications of RNAi to different model organisms for functional gene analysis, including nonclassical model systems such as the parasitic protozoan Trypanosoma brucei and the chicken embryo. In addition, several chapters are devoted to subjects that are more loosely related to RNAi and thus less frequently reviewed in papers in the field....RNAi is indeed obligatory reading for anyone interested in modern molecular biology.”
      —Science

  “This book provides a useful and relatively comprehensive summary of progress in the field, and it is likely that every biologist will find something of interest. While experts in this field will be able to update their knowledge on RNAi and its applications (for example, its use in high–throughput screening), the book should also serve as a useful introduction to the world of small RNAs for the non–expert. An added bonus is the inclusion of many protocols that explain the details of key RNAi experiments that once again should prove useful to the expert and non–expert alike.”
      —Nature Cell Biology