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Basic Methods in Microscopy
Protocols and Concepts from Cells: A Laboratory Manual

By David L. Spector, Cold Spring Harbor Laboratory; Robert D. Goldman, Northwestern University Medical School, Chicago

© 2006 • 382 pp., illus., index
Paperback • £61.00 • ISBN 978-087969751-8

Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manual Cells: A Laboratory Manual, provide a solid course in the basics of using the microscope in a biology laboratory.

Basic Methods in Microscopy provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events.

This manual is an important tool for any biology researcher employing imaging as a research method.

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Basic Methods in Protein Purification and Analysis: A Laboratory Manual

Edited By Richard J. Simpson, Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Peter D. Adams, Fox Chase Cancer Center, Philadelphia; Erica A. Golemis, Fox Chase Cancer Center, Philadelphia

© 2009 • 436 pp., illus., appendices, index
Hardcover • £102.00 • ISBN 978-087969868-3

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein–protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods.

The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein–Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts—a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein–protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases.

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Basic Methods in Protein Purification and Analysis: A Laboratory Manual

Edited By Richard J. Simpson, Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Peter D. Adams, Fox Chase Cancer Center, Philadelphia; Erica A. Golemis, Fox Chase Cancer Center, Philadelphia

© 2009 • 436 pp., illus., appendices, index
Paperback • £61.00 • ISBN 978-087969-867-6

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein–protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods.

The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein–Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts—a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein–protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases.

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Basic Stereology for Biologists and Neuroscientists

By Mark J. West, University of Aarhus, Denmark

© 2012 • 206 pp., illus., index
Hardcover • £44 • ISBN 978-1-936113-60-6

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Binding and Kinetics for Molecular Biologists

By James A. Goodrich, University of Colorado, Boulder; Jennifer F. Kugel, University of Colorado, Boulder

© 2007 • 182 pp., illus., appendices, index
Concealed wire binding • £39.00 • ISBN 978-087969736-5

This handbook offers a practical guide to the principles of quantitative analysis in biological experiments. The material is primarily aimed at working molecular biologists, but the scope and clarity of presentation make it equally suitable as an introduction for students. Topics covered range from the basics—such as measuring the concentrations of macromolecules—through considerations of binding constants and the kinetics of molecular interactions. The book ends with a thorough consideration of data analysis.

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Bioinformatics: Sequence and Genome Analysis, Second Edition

By David Mount, University of Arizona, Tucson

© 2004 • 665 pp., illus., appendices, index
Paperback • £60.00 • ISBN 978-087969712-9

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The Biology of Alzheimer Disease

Edited By Dennis J. Selkoe, Harvard Medical School; Eckhard Mandelkow, Max-Planck-Unit for Structural Molecular Biology; David M. Holtzman, Washington University School of Medicine

© 2011 • 511 pp., illus. (63 4C & 9 B&W), index
Hardcover • £85.00 • ISBN 978-1-936113-44-6

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Biology of Drosophila

Edited By M. Demerec

© 2008 • 632 pp., illus., indexes
Paperback • £27.00 • ISBN 978-087969828-7

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The Biology of Plants
(Cold Spring Harbor Symposia on Quantitative Biology LXXVII)

Edited By Terri Grodzicker, Cold Spring Harbor Laboratory; Robert Martienssen, Cold Spring Harbor Laboratory; David Stewart, Cold Spring Harbor Laboratory; Bruce Stillman, Cold Spring Harbor Laboratory

Due June 2013 • 440 pp. (approx.), illus., index
(Paperback edition does not include online access)
Paperback • £82 • ISBN 978-1-621820-26-0

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The Biology of Plants
(Cold Spring Harbor Symposia on Quantitative Biology LXXVII)

Edited By Terri Grodzicker, Cold Spring Harbor Laboratory; Robert Martienssen, Cold Spring Harbor Laboratory; David Stewart, Cold Spring Harbor Laboratory; Bruce Stillman, Cold Spring Harbor Laboratory

Due June 2013 • 440 pp. (approx.), illus., index
(Hardcover edition includes online access; call for information and registration)
Hardcover • £201 • ISBN 978-1-621820-25-3