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An Introduction to Nervous Systems

By Ralph J. Greenspan, The Neurosciences Institute, San Diego, California

© 2007 • 172 pp., illus., bibliography, glossary, index
Hardcover • £42.00 £21.00 • ISBN 978-087969757-0

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Angiogenesis: Biology and Pathology

Edited By Michael Klagsbrun, Children's Hospital Boston; Patricia D'Amore, Schepens Eye Research Institute

© 2011 • 522 pp., illus. (62 4C and 11 B&W), index
Hardcover • £85.00 • ISBN 978-1-936113-43-9

The Annotated and Illustrated Double Helix

By James D. Watson, Cold Spring Harbor Laboratory; Alexander Gann, Cold Spring Harbor Laboratory; Jan Witkowski, Cold Spring Harbor Laboratory

© 2013 • 345 pages, illus. (320 B&W), index
Hardcover • £Not for Sale • ISBN 978-1-476715-49-0

Antibodies: A Laboratory Manual

By Ed Harlow, Cold Spring Harbor Laboratory; David Lane, Imperial Cancer Research Fund Laboratories

© 1988 • 726 pp., illus., appendices, bibliography, index
Paper • £55.00 • ISBN 978-087969314-5

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At the Bench: A Laboratory Navigator, Updated Edition

By Kathy Barker, The Institute for Systems Biology, Seattle

© 2005 • 465 pp., illus., appendices, index
Concealed wire binding • £39.00 • ISBN 978-087969708-2

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At the Helm: Leading Your Laboratory, Second Edition

By Kathy Barker, Seattle, Washington

© 2010 • 372 pp., illus. (2 b/w), index
Trim size: 7” x 10”
Hardcover • £39.00 • ISBN 978-0-879698-66-9

Since 2002, the first edition of this best-selling book has helped thousands of newly appointed principal investigators successfully transition to running their own labs. But changes in technology continue to transform the way science is done, affecting ways in which labs communicate and collaborate, organize data and supplies, and keep current on the latest developments. The culture of science has also evolved, as more scientists explore non-academic career paths, seek new ways to communicate information and ideas, and acquire skills and knowledge outside of their field. In the second edition of this book, Kathy Barker has substantially revised the text, offering PIs advice on adapting to the changes and challenges that the years have brought. New topics include collaboration contracts, performance evaluations, communicating with non-scientists, tips for succeeding on the tenure track, and professional development. With this book as a guide, any new or aspiring PI will be well-equipped to manage personnel, time, and institutional responsibilities with confidence.

About the author: Kathy Barker received her B.A. in Biology and English, and her M.A. and Ph.D. in Microbiology, from various branches of the University of Massachusetts. She did her postdoctoral work in the laboratory of Viral Oncology at Rockefeller University and was an Assistant Professor in the Laboratory of Cell Physiology and Immunology at Rockefeller University. She is now based in Seattle, where she writes and gives workshops on various aspects of running a lab.

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Auxin Signaling: From Synthesis to Systems Biology

Edited By Mark Estelle, University of California, San Diego; Dolf Weijers, Wageningen University, The Netherlands; Karin Ljung, Umeå Plant Science Centre, Umeå, Sweden; Ottoline Leyser, University of York, United Kingdom

© 2011 • 253 pp., illus. (36 color, 13 b/w), index
Trim size: 7” x 10”
Hardcover • £85.00 • ISBN 978-0-879698-98-0

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Basic Methods in Microscopy
Protocols and Concepts from Cells: A Laboratory Manual

By David L. Spector, Cold Spring Harbor Laboratory; Robert D. Goldman, Northwestern University Medical School, Chicago

© 2006 • 382 pp., illus., index
Paperback • £61.00 • ISBN 978-087969751-8

Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manual Cells: A Laboratory Manual, provide a solid course in the basics of using the microscope in a biology laboratory.

Basic Methods in Microscopy provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events.

This manual is an important tool for any biology researcher employing imaging as a research method.

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Basic Methods in Protein Purification and Analysis: A Laboratory Manual

Edited By Richard J. Simpson, Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Peter D. Adams, Fox Chase Cancer Center, Philadelphia; Erica A. Golemis, Fox Chase Cancer Center, Philadelphia

© 2009 • 436 pp., illus., appendices, index
Paperback • £61.00 • ISBN 978-087969-867-6

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein–protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods.

The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein–Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts—a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein–protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases.

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Basic Methods in Protein Purification and Analysis: A Laboratory Manual

Edited By Richard J. Simpson, Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Peter D. Adams, Fox Chase Cancer Center, Philadelphia; Erica A. Golemis, Fox Chase Cancer Center, Philadelphia

© 2009 • 436 pp., illus., appendices, index
Hardcover • £102.00 • ISBN 978-087969868-3

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein–protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods.

The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein–Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts—a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein–protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases.