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382 pp., illus., index
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Basic Methods in Microscopy provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events.
This manual is an important tool for any biology researcher employing imaging as a research method.
In summary, this book is a useful reference for a teaching laboratory or a microscopy facility with very general interests in a variety of microscopy techniques primarily for fixed tissues.
Any research laboratory that uses microscopy would benefit immensely by having this volume on hand. The components, from underlying optical principles and theories behind the most basic light microscope to the highly advanced transmission electron microscope, are clearly described in the text, figures, and legends. A variety of techniques in fluorescence microscopy are presented, including protocols for the preparation of specimens, preparation of slides and cover slips, and proper care, maintenance, and cleaning of optical equipment. A handy troubleshooting section provides guidance on how to correct imaging problems.
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