© 2007 • 182 pp., illus., appendices, index
Concealed wire binding • $59 • ISBN  978-087969736-5

This handbook offers a practical guide to the principles of quantitative analysis in biological experiments. The material is primarily aimed at working molecular biologists, but the scope and clarity of presentation make it equally suitable as an introduction for students. Topics covered range from the basics—such as measuring the concentrations of macromolecules—through considerations of binding constants and the kinetics of molecular interactions. The book ends with a thorough consideration of data analysis.

  “This textbook provides a good introduction to the theory of binding kinetics. It supplies technical insight to proper affinity assay development. The volume is ideally suited for readers who seek background information prior to designing ligand-protein interaction assays.”
      —The Quarterly Review of Biology

1. Performing Quantitative Experiments with Biomolecules

2. Affinity Constants

3. Cooperativity in Binding

4. Rate Constants for Binding and Dissociation

5. Kinetics of Regulation and Cooperativity in Binding Reactions

6. Constants for a Two-step Binding Reaction

7. Data Analysis

Appendix 1: Equation Derivations

Appendix 2: Resources and References

Index