© 2008 • 242 pp., illustrations from original articles, indexes, CD
Hardcover • $39 • ISBN  978-087969804-1

This book is the second volume of an intellectual history of the science done at CSHL (the first volume, Illuminating Life, showed that genetics became the dominant theme of research at CSH by as early as 1904). The appointment of James Watson as Director of the Laboratory in 1968 set off the explosive research development at CSH, as he recruited widely and wisely teams of investigators with diverse scientific interests. From this collection of papers, presented in full on the accompanying CD, several themes emerge: the characterization and exploitation of mobile genetic elements; the mechanics of DNA replication and regulation of the cell cycle; the behavior and internal architecture of cells; how viruses induce tumors; the discovery of cancer genes; the characteristics of neurons; and the invention of techniques that make possible further progress. Each theme is introduced in the context of the science of the time, and each paper has a commentary by, in most cases, one of its authors. Life Illuminated is a story of scientific innovation and achievement, told in the words of the investigators themselves.

Preface

Jan A. Witkowski, Alexander Gann, and Joseph F. Sambrook

Acknowledgments

GENETICS

Gerald R. Fink

Introduction: Genes Are Interesting to Geneticists

Rasika Harshey

Ljungquist E. and Bukhari A.I. 1977. State of prophage Mu DNA upon induction. Proc. Natl. Acad. Sci. 74: 3143-3147.

Bukhari Memoriam

Taylor A.L. and Szybalski W. 1984. In memoriam: Ahmad I. Bukhari, 1943-1983. Gene 27: iii-iv.

James B. Hicks

Strathern J.N, Klar A.J.S., Hicks J.B., Abraham J.A., Ivy J.M., Nasmyth K.A., and McGill C. 1982. Homothallic switching of yeast mating type cassettes is initiated by a double-stranded cut in the MAT locus. Cell 31: 183-192.

Robert Martienssen

Sundaresan V., Springer P., Volpe T., Haward S., Jones J.D.G., Dean C., Ma H., and Martienssen R. 1995. Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements. Genes Dev. 9: 1797-1810.

David Beach

Draetta G., Luca F.,Westendorf J., Brizuela L., Ruderman J., and Beach D. 1989. cdc2 protein kinase is complexed with both cyclin A and B: Evidence for proteolytic inactivation of MPF. Cell 56: 829-838.

DNA

Bruce Alberts

Introduction: Why the Revolution That Jim Started Continues

Joseph F. Sambrook

Watson J.D. 1972. Origin of concatemeric T7 DNA. Nat. New Biol. 239: 197-201.

Bruce Stillman

Prelich G., Kostura M., Marshak D.R., Mathews M.B., and Stillman B. 1987. The cell cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro. Nature 326: 471-475.

Bruce Stillman

Bell S.P. and Stillman B. 1992. ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex. Nature 357: 128-134.

Richard J. Roberts

Klimašauskas S., Kumar S., Roberts R.J., and Cheng X. 1994. HhaI methyltransferase flips its target base out of the DNA helix. Cell 76: 357-369.

CELL BIOLOGY

David L. Spector

Introduction: From Architecture to Functional Analysis

Klaus Weber

Lazarides E. and Weber K. 1974. Actin antibody: The specific visualization of actin filaments in non-muscle cells. Proc. Natl. Acad. Sci. 71: 2268-2272.

Guenter Albrecht-Buehler

Albrecht-Buehler G. 1977. Daughter 3T3 cells. Are they mirror images of each other? J. Cell Biol. 72: 595-603.

Dafna Bar-Sagi and James R. Feramisco

Bar-Sagi D. and Feramisco J.R. 1985. Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. Cell 42: 841-848.

Carol W. Greider

Harley C.B., Futcher A.B., and Greider C.W. 1990. Telomeres shorten during ageing of human fibroblasts. Nature 345: 458-460.

David L. Spector

Spector D.L. 1990. Higher order nuclear organization: Three-dimensional distribution of small nuclear ribonucleoprotein particles. Proc. Natl. Acad. Sci. 87: 147-151.

TUMOR VIRUSES

Joseph F. Sambrook

Introduction: The Early Days of Tumor Virus Research at Cold Spring Harbor

Joseph F. Sambrook

Sambrook J., Sharp P.A., and Keller W. 1972. Transcription of simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells. J. Mol. Biol. 70: 57-71.

Terri Grodzicker

Grodzicker T., Williams J., Sharp P., and Sambrook J. 1974. Physical mapping of temperature-sensitive mutations of adenoviruses. Cold Spring Harbor Symp. Quant. Biol. 39(Pt 1): 439-446.

Louise T. Chow

Chow L.T., Gelinas R.E., Broker T.R., and Roberts R.J. 1977. An amazing sequence arrangement at the 5’ ends of adenovirus 2 messenger RNA. Cell 12: 1-8.

Michael Botchan

Botchan M., Topp W., and Sambrook J. 1976. The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell 9: 269-287.

Robert Tjian

Tjian R. 1978. The binding site on SV40 DNA for a T antigen-related protein. Cell 13: 165-179.

Joseph F. Sambrook

Gluzman Y. 1981. SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23: 175-182.

Carl S. Thummel

Thummel C., Tjian R., and Grodzicker T. 1981. Expression of SV40 T antigen under control of adenovirus promoters. Cell 23: 825-836.

Winship Herr

Herr W. and Clarke J. 1986. The SV40 enhancer is composed of multiple functional elements that can compensate for one another. Cell 45: 461-470.

NEUROSCIENCE

Eric Kandel

Introduction: Neuroscience at CSH Laboratory: Meetings, Courses, and Research

Ron McKay

Introduction: Getting the Point at Cold Spring Harbor

Birgit Zipser

Zipser B. and McKay R. 1981. Monoclonal antibodies distinguish identifiable neurons in the leech. Nature 289: 549-554.

Ron McKay

Hockfield S. and McKay R.D.G. 1985. Identification of major cell classes in the developing mammalian nervous system. J. Neurosci. 5: 3310-3328.

Tim Tully

Yin J.C.P.,Wallach J.S., Del Vecchio M.,Wilder E.L., Zhou H., Quinn W.G., and Tully T. 1994. Induction of a dominant negative CREB transgene specifically blocks longterm memory in Drosophila. Cell 79: 49-58.

CANCER

Arnold J. Levine

Introduction: The Road to Understanding the Origins of Cancer in Humans

Mitchell Goldfarb

Goldfarb M., Shimizu K., Perucho M., and Wigler M. 1982. Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells. Nature 296: 404-409.

Earl Ruley

Ruley H.E. 1983. Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture. Nature 304: 602-606.

Scott Powers

Kataoka T., Powers S., Cameron S., Fasano O., Goldfarb M., Broach J., and Wigler M. 1985. Functional homology of mammalian and yeast RAS genes. Cell 40: 19-26.

Douglas Hanahan

Hanahan D. 1985. Heritable formation of pancreatic β-cell tumours in transgenic mice expressing recombinant insulin/simian virus 40 oncogenes. Nature 315: 115-122.

B. Robert Franza, Jr.

Franza B.R. Jr., Rauscher F.J. 3rd, Josephs S.F., and Curran T. 1988. The Fos complex and Fos-related antigens recognize sequence elements that contain AP-1 binding sites. Science 239: 1150-1153.

Ed Harlow

Whyte P., Buchkovich K.J., Horowitz J.M, Friend S.H., Raybuck M., Weinberg R.A., and Harlow E. 1988. Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product. Nature 334: 124-129.

TECHNIQUES

Angela N.H. Creager

Introduction: Early Technological Breakthroughs at Cold Spring Harbor

Richard J. Roberts

Roberts R.J. 1978. Restriction and modification enzymes and their recognition sequences. Gene 4: 183-194.

James I. Garrels

Garrels J.I. 1989. The QUEST system for quantitative analysis of two-dimensional gels. J. Biol. Chem 264: 5269-5282.

Joseph F. Sambrook

Sharp P.A., Sugden B., and Sambrook J. 1973. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresis. Biochemistry 12: 3055-3063.

Tom Maniatis

Maniatis T., Kee S.G., Efstratiadis A., and Kafatos F.C. 1976. Amplification and characterization of a β-globin gene synthesized in vitro. Cell 8: 163-182.

Author Index

Index