© 2003 • 520 pp., illus., appendices, index
Paperback • ISBN  978-087969654-2

This second edition of a much–praised and widely used manual has been entirely revised and updated. Each technique is presented with extensive background information, advice, and troubleshooting. All current applications of PCR are covered, in protocols that have the hallmark reliability of the previous edition.

  “Most chapters contain nice graphics, and all feature multiple detailed protocols and recipes. Very useful (besides the index and the over 600 references) are the chapters that compare products—for example, thermostable polymerases or real–time PCR systems. This manual contains a wealth of tips and protocols that will inspire researchers or at least save them time.”
      —The Quarterly Review of Biology

Section 1. Introduction to PCR

Section 2. Sample Preparation

Section 3. RT-PCR Methods

Section 4. Detection of PCR Products: Specialized Applications

Section 5. Analysis of Differential Gene Expression by PCR

Section 6. Cloning by PCR

Section 7. Cloning of PCR Products

Section 8. Mutagenesis by PCR

Appendices