Microproteomics, Immunoimaging featured in Cold Spring Harbor Protocols|
COLD SPRING HARBOR, N.Y. (Tues., Feb. 1, 2011) – Comparative and quantitative proteomic technologies have not progressed to the extent of their genomic and transcriptomic counterparts. Unlike the genome, which is essentially identical in the somatic cells of a given organism, the proteome varies in different cells, and there is no self-replicating amplification mechanism for proteins such as the polymerase chain reaction (PCR) for DNA. Because of this, methods that extract, separate, detect and identify proteins from extremely small samples are needed. In the February issue of Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/TOCs/toc2_11.dtl), Duke University’s Erich Jarvis and colleagues (http://www.jarvislab.net/) provide such a method, Microproteomics: Quantitative Proteomic Profiling of Small Numbers of Laser-Captured Cells. The protocol uses laser capture microdissection to isolate pure cell populations from tissue sections and nanoscale liquid chromatography/tandem mass spectrometry to simultaneously identify and quantify hundreds of proteins from samples as small as 1,000 cells. The article is freely available on the journal’s website (http://cshprotocols.cshlp.org/cgi/content/full/2011/2/pdb.prot5573).
Immunoimaging is rapidly developing from a merely descriptive technique into a set of methods and analytical tools that can be used to quantify and characterize an immune response at the cellular level. In the February issue of Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/TOCs/toc2_11.dtl), Ian Parker and colleagues from the University of California, Irvine http://parkerlab.bio.uci.edu/members/ianparker.htm) present Immunoimaging: Studying Immune System Dynamics Using Two-Photon Microscopy. The article outlines the hardware required for immunoimaging and discusses methods for quantitative analysis of multidimensional image stacks. This overview article is freely available on the journal’s website (http://cshprotocols.cshlp.org/cgi/content/full/2011/2/pdb.top99), and the issue also contains protocols from the same authors for a General Approach to Adoptive Transfer and Cell Labeling for Immunoimaging (http://cshprotocols.cshlp.org/cgi/content/full/2011/2/pdb.prot5565), Induction of an Immune Response for Imaging Antigen-Presenting Cell/T-Cell Interactions (http://cshprotocols.cshlp.org/cgi/content/abstract/2011/2/pdb.prot5566), In Situ Lymph Node Imaging (http://cshprotocols.cshlp.org/cgi/content/abstract/2011/2/pdb.prot5567) and In Vivo Lymph Node Imaging (http://cshprotocols.cshlp.org/cgi/content/abstract/2011/2/pdb.prot5568).
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Cold Spring Harbor Protocols (www.cshprotocols.org) is a monthly peer-reviewed journal of methods used in a wide range of biology laboratories. It is structured to be highly interactive, with each protocol cross-linked to related methods, descriptive information panels, and illustrative material to maximize the total information available to investigators. Each protocol is clearly presented and designed for easy use at the bench—complete with reagents, equipment, and recipe lists. Life science researchers can access the entire collection via institutional site licenses, and can add their suggestions and comments to further refine the techniques.
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