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Molecular Cloning: A Laboratory Manual, Fourth Edition

The most celebrated lab manual in molecular biology has been updated and expanded.

Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems.

For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.

Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled.

The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes.

Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standard—the one indispensable molecular biology laboratory manual and reference source.

•  Extensive new content: 12 entirely new chapters are devoted to the most exciting current research strategies, including epigenetic analysis, RNA interference, genome sequencing, and bioinformatics
•  Expanded scope: the nucleic-acid-based techniques selected for inclusion have promoted recent advances in gene transfer, protein expression, RNA analysis, and expression of cloned genes
•  Classic content: 10 original core chapters have been updated to reflect developments and innovations in standard techniques and to introduce new cutting-edge protocols

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About the book:
Molecular Cloning: A Laboratory Manual, Fourth Edition (ISBN: 978-1-936113-41-5 cloth; 978-1-936113-42-2 paper) was written by Michael R. Green and Joseph Sambrook and published by Cold Spring Harbor Laboratory Press. The three-volume set is available in hardcover and paperback and is 2,088 pp. in length. For more information, see http://www.cshlpress.com/link/molclon4p.htm.

About the authors:
Michael R. Green is a distinguished HHMI investigator at the University of Massachusetts Medical School and Joseph Sambrook, the celebrated founding author of Molecular Cloning at Peter MacCallum Cancer Institute, Melbourne, Australia.

About Cold Spring Harbor Laboratory Press:
Cold Spring Harbor Laboratory Press is an internationally renowned publisher of books, journals, and electronic media, located on Long Island, New York. Since 1933, it has furthered the advance and spread of scientific knowledge in all areas of genetics and molecular biology, including cancer biology, plant science, bioinformatics, and neurobiology. It is a division of Cold Spring Harbor Laboratory, an innovator in life science research and the education of scientists, students, and the public. For more information, visit www.cshlpress.com.