` CSHLPress.com - organ cell culture methods
Search  (type two or more letters for suggestions): Or: Advanced Search

CSHL Press News

Cold Spring Harbor Protocols features organ and cell culture methods


Cold Spring Harbor Protocols features organ and cell culture methods

COLD SPRING HARBOR, N.Y. (Mon., Nov. 3, 2008) – Understanding the function of organs like the brain, kidney and reproductive tissues requires experimental systems that allow for the study and manipulation of developing cells and tissues in the laboratory. This month’s issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc11_08.dtl) features two articles with detailed instructions for setting up these experimental culture systems. Both methods are derived from Cold Spring Harbor Laboratory’s renowned Molecular Embryology of the Mouse course (meetings.cshl.edu/courses/c-mous09.shtml).

Neural progenitor cells, which are destined to contribute to the nervous system, are often difficult to identify. Isolation, Culture and Differentiation of Progenitor Cells from the Central Nervous System, from Larysa Pevny’s laboratory at the University of North Carolina (www.unc.edu/~pevny/), describes the Neurosphere Assay, which allows one to identify neural progenitor cells as well as to determine both their self-renewal capacity and their ability to generate three primary cell types of the nervous system: neurons, astrocytes and oligodendrocytes. The protocol is freely accessible on the website for Cold Spring Harbor Protocols (www.cshprotocols.org/cgi/content/full/2008/12/pdb.prot5077).

Blanche Capel’s laboratory at Duke University (www.cellbio.duke.edu) provides Preparing Recombinant Gonad Organ Cultures. It can be useful to assay migration between any two adjacent tissues during development. This protocol assays cell migration between the developing gonad and kidney using tissue recombination, combined with an organ culture technique. This method is freely accessible on the website for Cold Spring Harbor Protocols (www.cshprotocols.org/cgi/content/full/2008/12/pdb.prot5078).

# # #

About Cold Spring Harbor Protocols:
Cold Spring Harbor Protocols (www.cshprotocols.org) is a monthly peer-reviewed journal of methods used in a wide range of biology laboratories. It is structured to be highly interactive, with each protocol cross-linked to related methods, descriptive information panels, and illustrative material to maximize the total information available to investigators. Each protocol is clearly presented and designed for easy use at the bench—complete with reagents, equipment, and recipe lists. Life science researchers can access the entire collection via institutional site licenses, and can add their suggestions and comments to further refine the techniques.

About Cold Spring Harbor Laboratory Press:
Cold Spring Harbor Laboratory Press is an internationally renowned publisher of books, journals, and electronic media, located on Long Island, New York. Since 1933, it has furthered the advance and spread of scientific knowledge in all areas of genetics and molecular biology, including cancer biology, plant science, bioinformatics, and neurobiology. It is a division of Cold Spring Harbor Laboratory, an innovator in life science research and the education of scientists, students, and the public. For more information, visit www.cshlpress.com.

For content and submission information:
David Crotty (crotty@cshl.edu; 516-422-4007), Executive Editor, Cold Spring Harbor Protocols

For access, subscription, and free trial information:
Stephanie Novara (novara@cshl.edu;516-422-4159), Journals Marketing Manager, CSHL Press

Return to CSHL Press News index
CSH Symposia Archive Our Books on the Blogosphere GrrlScientist on Experimental Heart
Photographer Timothy Archibald on My Heart vs. The Real WOrld
The hottest jobs in science, and how to land them
New Evolution Textbook Podcasts
Georgina Ferry on Max Perutz and The Secret of Life
Editor’s Select -- Free Articles From the Current Issues of our Journals
CSH Perspectives

Cold Spring Harbor Perspectives

Defining left and right in developing embryos — the view from Nobutaka Hirokawa’s laboratory.
Nobutaka Hirokawa et al.

CSH Protocols logo art

Cold Spring Harbor Protocols

Genes and Development cover

Genes & Development

Switching of the core transcription machinery during myogenesis.
Maria Divina E. Deato and Robert Tjian
Vol. 21 (17) 2137–2149.

A tDNA establishes cohesion of a neighboring silent chromatin domain.
Rudra N. Dubey and Marc R. Gartenberg
Vol. 21 (17) 2150–2160.

Gennome Research cover

Genome Research

Learning and Memory cover

Learning & Memory

Hippocampal differentiation without recognition: An fMRI analysis of the contextual cueing task.
Anthony J. Greene, William L. Gross, Catherine L. Elsinger, and Stephen M. Rao
Vol. 14 (8) 548–553.

RNA cover


Elongation factor G stabilizes the hybrid-state conformation of the 70S ribosome.
P. Clint Spiegel, Dmitri N. Ermolenko, and Harry F. Noller
Vol. 13 (9) 1473–1482.