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The Nucleus, Second Edition

Subject Area(s):  Cell BiologyMolecular BiologyBiochemistry

Edited by Ana Pombo, Max Delbrück Center for Molecular Medicine; Martin W. Hetzer, Salk Institute for Biological Studies; Tom Misteli, National Cancer Institute, NIH

Download a Free Excerpt from The Nucleus, Second Edition:

Physical Nature of Chromatin in the Nucleus

© 2022 • 522 pages, illustrated (90 color and 2 B&W), index
Hardcover •
ISBN  978-1-621823-89-6

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  •     Description    
  •     Contents    


The nucleus is the most prominent structure in eukaryotic cells. It houses the cell’s DNA and is the hub for DNA replication, transcription, and RNA processing. Despite its prominence and importance, our understanding of how the nucleus and its DNA are organized in space and time—and the implications of that organization for proper function—has lagged behind that of other cellular structures.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology covers recent advances in our understanding of nuclear organization and function. The contributors discuss the 3D organization of chromatin, the various nuclear bodies and compartments that have been identified, and the roles of RNA and actin in shaping nuclear organization, as well as how these structures interact with each other and with peripheral features (e.g., the nuclear pore complex and inner nuclear membrane proteins) to carry out the work of the nucleus. Insights into DNA replication timing and RNA processing dynamics based on new technologies aimed at examining chromatin and other intranuclear structures at high resolution are also included.

Multiple chapters are devoted to physiological and disease processes involving disruption of nuclear structure and function (e.g., viral infection). This volume is therefore essential reading for all cell and molecular biologists, as well as pathologists interested in the role of nuclear architecture in disease.


The Nuclear Lamina
Xianrong Wong, Ashley J. Melendez-Perez, and Karen L. Reddy
Structure, Maintenance, and Regulation of Nuclear Pore Complexes: The Gatekeepers of the Eukaryotic Genome
Marcela Raices and Maximiliano A. D'Angelo
The Nuclear Pore Complex as a Transcription Regulator
Michael Chas Sumner and Jason Brickner
The Diverse Cellular Functions of Inner Nuclear Membrane Proteins
Sumit Pawar and Ulrike Kutay
Mechanical Forces in Nuclear Organization
Yekaterina A. Miroshnikova and Sara A. Wickström
Liquid–Liquid Phase Separation in Chromatin
Karsten Rippe
The Stochastic Genome and Its Role in Gene Expression
Christopher H. Bohrer and Daniel R. Larson
Emerging Properties and Functions of Actin and Actin Filaments Inside the Nucleus
Svenja Ulferts, Bina Prajapati, Robert Grosse, and Maria K. Vartiainen
Physical Nature of Chromatin in the Nucleus
Kazuhiro Maeshima, Shiori Iida, and Sachiko Tamura
Mechanisms of Chromosome Folding and Nuclear Organization: Their Interplay and Open Questions
Leonid Mirny and Job Dekker
Uncovering the Principles of Genome Folding by 3D Chromatin Modeling
Asli Yildirim, Lorenzo Boninsegna, Yuxiang Zhan, and Frank Alber
Transcription Factor Dynamics
Feiyue Lu and Timothée Lionnet
Essential Roles for RNA in Shaping Nuclear Organization
Sofia A. Quinodoz and Mitchell Guttman
Nuclear Compartments: An Incomplete Primer to Nuclear Compartments, Bodies, and Genome Organization Relative to Nuclear Architecture
Andrew S. Belmont
The Impact of Space and Time on the Functional Output of the Genome
Marcelo Nollmann, Isma Bennabi, Markus Götz, and Thomas Gregor
Mammalian DNA Replication Timing
Athanasios E. Vouzas and David M. Gilbert
Imaging Organization of RNA Processing within the Nucleus
Jeetayu Biswas, Weihan Li, Robert H. Singer, and Robert A. Coleman
Chromatin Mechanisms Driving Cancer
Berkley Gryder, Peter C. Scacheri, Thomas Ried, and Javed Khan
Viruses in the Nucleus
Bojana Lucic, Ines J. de Castro, and Marina Lusic
3D or Not 3D: Shaping the Genome during Development
Juliane Glaser and Stefan Mundlos
Epigenetic Reprogramming in Early Animal Development
Zhenhai Du, Ke Zhang, and Wei Xie
The Molecular and Nuclear Dynamics of X-Chromosome Inactivation
François Dossin and Edith Heard
Organization of the Pluripotent Genome
Patrick S.L. Lim and Eran Meshorer