The first edition of Mark Ptashne's 1986 book describing the principles of gene regulation in phage lambda became a classic in both content and form, setting a standard of clarity and precise prose that has rarely been bettered. This edition is a reprint of the original text, together with a new chapter updating the story to 2004. Among the striking new developments are recent findings on long–range interactions between proteins bound to widely separated sites on the phage genome, and a detailed description of how gene activation works.
Author
Mark Ptashne
Sloan-Kettering Memorial Cancer Center, New York
Publication Date
2004/2004
Bibliographic Information 154 pp., illus., appendices, index
Twenty-four true, wide-ranging tales of crime, history, human behavior, illness, and ethics, told from the personal perspective of the author, an eminent physician-lawyer who uses the stories to illustrate the principles of human genetics and to discuss the broader issues.
Author
Philip R. Reilly
Interleukin Genetics and Tufts University School of Medicine
Publication Date
2000/2000
Bibliographic Information 339 pp., illus., indexes, references
Twenty-four true, wide-ranging tales of crime, history, human behavior, illness, and ethics, told from the personal perspective of the author, an eminent physician-lawyer who uses the stories to illustrate the principles of human genetics and to discuss the broader issues.
Author
Philip R. Reilly
Interleukin Genetics and Tufts University School of Medicine
Publication Date
September 2000/2000
Bibliographic Information 339 pp., illus., indexes, references
The idea that the adult brain of mammals can generate new neurons has only recently been accepted by the scientific community, and research in this exciting area is now in full swing. Bringing together leading researchers in the field of adult neurogenesis, the 30 chapters in this monograph provide a valuable overview of this emerging field and lay the groundwork for future studies. Adult Neurogenesis includes discussions on neural stem cell biology; methods and models for studying adult neurogenesis; physiological and molecular processes and their control; related neurological diseases; and comparisons of neurogenesis in humans, birds, fish, and invertebrates. It will be of interest to all researchers in neurobiology as well as those in the medical field, as it has implications for understanding depression, epilepsy, and other psychiatric disorders.
Editor
Fred H. Gage
The Salk Institute for Biological Studies, San Diego, California
Ageless Quest is a personal, sometimes controversial, account of the pursuit of a genetic ‘cure’ for aging by an expert in the field.
The author is the Novartis Professor of Biology at the Massachusetts Institute of Technology.
Aging has always been regarded as a highly complex process with many degenerative changes leading to the cessation of life. But recent research has identified a relatively simple mechanism that governs the pace of aging.
Lenny Guarente's Ageless Quest is a scientific detective story for the baby boom generation. It offers an insider's view of an area of potentially astonishing high reward—and equally high risk.
To read Lenny Guarente’s and Robert Butler’s interview with Sara Davidson (The NY Times) about how the body ages and the research on trying to extend our healthy life span, click here. (You must register for free access to NYTimes.com)
Every human being is unique. You are made up of 23 chromosomes from your mother and 23 chromosomes from your father, and those 46 chromosomes contain a unique mixture of genes. That same mixture is in the millions of cells that make up your body. But although about 99.5% of your genes are the same as everybody else's, some parts vary: hair color, shape of ears, color of skin. And some people inherit genetic diseases like cystic fibrosis. How does that happen? Read this book to discover some amazing facts about your genes. (Ages 9-15)
Author
Fran Balkwill
Contributor
Mic Rolph
Publication Date
JANUARY 1991/1993
Bibliographic Information 32 fully illustrated 4-color pages
Medical science constantly demands our attention, as patients or relatives, concerned citizens, voters, investors, or simply curious individuals. But for those without training, the language of science is often hard to follow. The A to Z of DNA Science book series defines and illustrates specialized terms in ways that non-specialists can appreciate and enjoy. This volume focuses on the language of genes, genomes, DNA, biotechnology, and heredity, defining, explaining, and illustrating over 200 terms used in books, broadcasting, websites, and newspaper and magazine articles.
Author
Jeffre L. Witherly
National Human Genome Research Institute, Bethesda, Maryland
Medical science constantly demands our attention, as patients or relatives, concerned citizens, voters, investors, or simply curious individuals. But for those without training, the language of science is often hard to follow. The A to Z of DNA Science book series defines and illustrates specialized terms in ways that non-specialists can appreciate and enjoy. This volume focuses on the language of genes, genomes, DNA, biotechnology, and heredity, defining, explaining, and illustrating over 200 terms used in books, broadcasting, websites, and newspaper and magazine articles.
Author
Jeffre L. Witherly
National Human Genome Research Institute, Bethesda, Maryland
The Illustrated Chinese–English Guide for Biomedical Scientists is intended to build confidence in the use of English scientific language. The book lists terms that are in common use in science laboratories, translated into both simplified and complex Chinese. It also contains illustrations of equipment, labeled in both languages.
Author
James M. Samet
Publication Date
2004/2004
Bibliographic Information 115 pp., illus., illustrations index
The Illustrated Chinese–English Guide for Biomedical Scientists is intended to build confidence in the use of English scientific language. The book lists terms that are in common use in science laboratories, translated into both simplified and complex Chinese. It also contains illustrations of equipment, labeled in both languages.
Author
James M. Samet
Publication Date
2004/2004
Bibliographic Information 115 pp., illus., illustrations index
An Introduction to Nervous Systems presents the principles of neurobiology from an evolutionary perspective—from single–celled organisms to complex invertebrates such as flies—and is ideal for use as a supplemental textbook. Greenspan describes the mechanisms that allow behavior to become ever more sophisticated—from simple avoidance behavior of Paramecium through to the complex cognitive behaviors of the honeybee—and shows how these mechanisms produce the increasing neural complexity found in these organisms. The book ends with a discussion of what is universal about nervous systems and what may be required, neurobiologically, to be human. This novel and highly readable presentation of fundamental principles of neurobiology is designed to be accessible to undergraduate and graduate students not already steeped in the subject.
Author
Ralph J. Greenspan
The Neurosciences Institute, San Diego, California
Publication Date
2007/2007
Bibliographic Information 172 pp., illus., bibliography, glossary, index
An Introduction to Nervous Systems presents the principles of neurobiology from an evolutionary perspective—from single–celled organisms to complex invertebrates such as flies—and is ideal for use as a supplemental textbook. Greenspan describes the mechanisms that allow behavior to become ever more sophisticated—from simple avoidance behavior of Paramecium through to the complex cognitive behaviors of the honeybee—and shows how these mechanisms produce the increasing neural complexity found in these organisms. The book ends with a discussion of what is universal about nervous systems and what may be required, neurobiologically, to be human. This novel and highly readable presentation of fundamental principles of neurobiology is designed to be accessible to undergraduate and graduate students not already steeped in the subject.
Author
Ralph J. Greenspan
The Neurosciences Institute, San Diego, California
Publication Date
2007/2007
Bibliographic Information 172 pp., illus., bibliography, glossary, index
This volume, which presents the results of a Cold Spring Harbor Banbury Center meeting, summarizes aspects of the molecular and cell biology, pathophysiology, and therapeutic use of angiogenic factors and inhibitors. Researchers, clinicians, and students will find this concise survey exceptionally helpful in integrating the current knowledge available from the wide spectrum of areas concerned with angiogenesis and in understanding future progress toward elucidating this experimentally and clinically interesting system.
Recent progress in cell and molecular biology has profound implications for understanding the developmental biology of mammalian embryos. The unifying theme of this volume is a cellular and molecular approach to gametogenesis, embryogenesis, and maternal-fetal interactions in the developmental biology of agriculturally significant mammals. Great progress has been achieved in our understanding of preimplantation mammalian embryogenesis in laboratory species, particularly the mouse, through application of cell and embryo culture methods, cell biological techniques, and, more recently, recombinant DNA technology. A major remaining opportunity is the application of these techniques to problems in domestic species, such as improved methods for in vitro growth and maturation of oocytes, egg and embryo cryopreservation, and genetic manipulation to enhance productivity and herd quality. The authors review current knowledge in key areas, emphasizing new approaches to unsolved problems in the developmental biology of domestic species.
Cell death in vivo, like cell replication, is a normal and continuous process with complex physiological controls. The form of programmed cell death known as apoptosis has become an intense focus of investigation in cancer biology, virology, immunology, neuroscience, and aging research. Understanding the events involved at a molecular level may permit their manipulation for therapeutic purposes. In this sequel to their pioneering 1991 book Apoptosis: The Molecular Basis of Cell Death, Tomei and Cope have assembled an entirely new collection of articles which describe the genetic control of apoptosis, and its regulation in normal and neoplastic cells of many types. This is an essential compilation of up-to-date research on an emerging topic of central concern in many fields.
Editor
L. David Tomei
LXR Biotechnology Inc., Richmond,California
Publication Date
April 1994/1994
Bibliographic Information 430 pp., illus., color plates, index
The cruciferous weed Arabidopsis thialiana has become a model system for the study of an unusually wide variety of aspects of plant biology. As a result, nearly all aspects of Arabidopsis biology are now under investigation, more scientists are involved, and plant science as a whole has been invigorated.
This book is the first comprehensive account of what is known about the organism. The information is presented in the context of plant biology in general, with the properties of Arabidopsis mutants and the insights derived from their analysis as a unifying theme. The book's scope includes genetics, growth and development, biochemistry, physiology, and responses to pathogens and environmental stress.
This unique volume, in the classic tradition of the Cold Spring Harbor Monograph Series, is a landmark in plant science, essential reading for investigators at graduate student level and beyond, and a work of reference that will serve the field for years to come.
Editor
Elliot M. Meyerowitz
California Institute of Technology
Publication Date
December 1994/1994
Bibliographic Information 1270 pp., illus., color plates, appendices, index
The thale cress Arabidopsis thaliana is increasingly popular among plant scientists: it is small, easy to grow, and makes flowers, and the sequence of its small and simple genome was recently completed. This is the most complete and authoritative laboratory manual to be published on this model organism and the first to deal with genomic and proteomic approaches to its biology.
Author
Detlef Weigel
Salk Institute Plant Biology Laboratoryand Max Planck Institute forDevelopmental Biology
Publication Date
2002/2002
Bibliographic Information 354 pp., appendices, index
The thale cress Arabidopsis thaliana is increasingly popular among plant scientists: it is small, easy to grow, and makes flowers, and the sequence of its small and simple genome was recently completed. This is the most complete and authoritative laboratory manual to be published on this model organism and the first to deal with genomic and proteomic approaches to its biology.
Author
Detlef Weigel
Salk Institute Plant Biology Laboratoryand Max Planck Institute forDevelopmental Biology
Publication Date
2002/2002
Bibliographic Information 354 pp., appendices, index
The use of ribosomal RNA sequences as molecular chronometers has led to the recognition of a group of prokaryotes, the Archaea, that are phylogenetically distinct from both bacteria and eukaryotes. Although the Archaea are prokaryotes, lacking a nuclear membrane and possessing a single circular chromosome, they possess several molecular properties with similarity to the eukaryotes such as transcription signals, transcription factors, chaperones, and histones. Their unique phylogeny is underscored by a predilection for extreme environments which include high temperature, high salt, and strictly anaerobic conditions and the adaptive strategies that they have evolved to survive in these environments. These unique biomolecular adaptations have recently generated interest in the areas of genetic variability and genome evolution, extremely thermostable enzymes, such as DNA polymerases for PCR, methanogenesis as an alternative fuel source, anaerobic bioremediation, and use of bacteriorhodopsin for production of a biocomputer chip or a model for transmembrane ion translocation.
The Archaea occupy a pivotal phylogenetic position and offer extraordinary potential for biotechnology. One of the difficulties facing researchers, especially newcomers who wish to study the Archaea, has been the lack of a comprehensive source for the specialized techniques required. As the field has evolved, solutions to many problems have been discovered empirically and the special techniques have largely been disseminated either verbally or by means of journal articles. The protocols in these three books are selected to provide a detailed guide to experiments with the methanogenic, extremely halophilic, and thermophilic sulfur-utilizing Archaea, with overviews to highlight areas of future development. The individual protocols consist of an introduction describing the specific applications of the techniques, step-by-step procedures for applying the protocols, followed by any additional comments that will facilitate successful application of the protocol. A feature of research in this area is the interplay between microbiology, bioengineering, bio-chemistry, and molecular biology, and authors from all of these fields have been selected to provide these three concise and comprehensive resources for scientists interested in conducting research on the Archaea.
Volume 1: Halophilic Archaea
Volume 2: Methanogenic Archaea
Volume 3: Thermophilic Archaea
Editor
Frank T. Robb (Editor in Chief)
University of Maryland BiotechnologyInstitute
Publication Date
September 1995/1995
Bibliographic Information 1037 pp., illus., appendices, index
A research laboratory filled with competent, busy people entirely familiar with its arcane customs and practices is a daunting place for newcomers. Kathy Barker knows this world. She was a technician, an undergraduate, then a graduate student at the University of Massachusetts, and as a postdoctoral fellow and assistant professor at Rockefeller University, she was a mentor to grad students, physicians in training, technicians, and research nurses. From this rich experience, she has written At the Bench, a unique handbook for living and working in the laboratory. Much more than a simple primer or lab manual, this book is an essential aid to understanding:
how research groups work at a human level—and how to fit in
what equipment is essential, and how to use it properly
how to get started and get organized
how to set up an experiment
how to handle and use data and reference sources
how to present yourself and your results—in print and in person
Wise, light-hearted, but thoroughly practical, Dr. Barker offers advice, moral support, social etiquette, and professional reassurance along with assume-nothing, step-by-step instructions for those basic but vital laboratory procedures that experienced investigators know—but may not realize novices don’t.
If you are a graduate student, a physician with research intentions, or a laboratory technician, this book is indispensable. If you have to manage or mentor such people, giving a copy to each of them will greatly improve your life, and theirs.
Author
Kathy Barker
Rockefeller University, New York
Publication Date
July 1998/1998
Bibliographic Information 460 pp., glossary, index
At the Bench is the unique and hugely successful handbook for living and working in the laboratory, an essential aid to understanding basic lab techniques and how research groups work at a human level. In this newly revised edition, chapters have been rewritten to accommodate the impact of computer technology and the Internet, not only on the acquisition and analysis of data, but also on its organization and presentation. Alternatives to the use of radiation have been expanded, and figures and illustrations have been redrawn to reflect changes in laboratory equipment and procedures.
Author
Kathy Barker
The Institute for Systems Biology, Seattle
Publication Date
2005/2005
Bibliographic Information 465 pp., illus., appendices, index
Newly appointed principal research investigators have to recruit, motivate, and lead a research team, manage personnel and institutional responsibilities, and compete for funding, while maintaining the outstanding scientific record that got them their position in the first place. Small wonder, then, that many principal investigators feel ill-prepared. In this book, a successor to her best-selling manual for new recruits to experimental science, At The Bench, Kathy Barker provides a guide for newly appointed leaders of research teams, and those who aspire to that role. With extensive use of interviews and a text enlivened with quotes and real-life examples, Dr. Barker discusses a wide range of management challenges and the skills that promote success. Her book is a unique and much-needed contribution to the literature of science.
This full-color atlas graphically documents the main events of embryonic and post-embryonic development in Drosophila. Schematic surface views and transverse sections from several developmental stages are shown for the individual organs such as gut, nervous system, epidermis and musculature. By combining camera lucida tracing with digital technology, Volker Hartenstein has created a unique, beautiful and convenient reference book that will interest all developmental biologists and is a must for the personal library of anyone working on fly biology.
Author
Volker Hartenstein
University of California, Los Angeles
Publication Date
April 1995/1993
Bibliographic Information 58 pp., 50 full color illustrations, index
Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manual Cells: A Laboratory Manual, provide a solid course in the basics of using the microscope in a biology laboratory.
Basic Methods in Microscopy provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events.
This manual is an important tool for any biology researcher employing imaging as a research method.
Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manual Cells: A Laboratory Manual, provide a solid course in the basics of using the microscope in a biology laboratory.
Basic Methods in Microscopy provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events.
This manual is an important tool for any biology researcher employing imaging as a research method.
Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein–protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods.
The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein–Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts—a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein–protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases.
Editor
Richard J. Simpson
Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Publication Date
2009/2009
Bibliographic Information 436 pp., illus., appendices, index
Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein–protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods.
The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein–Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts—a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein–protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases.
Editor
Richard J. Simpson
Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Publication Date
2009/2009
Bibliographic Information 436 pp., illus., appendices, index
On Becoming a Scientist is a fast-paced "MTV-style" video showing a "day in the life" of three graduate students and a laboratory manager. These young scientists are followed as they work in their laboratories, take part in UCSF programs for public school children and the homeless, and socialize. Through interviews with scientists and students, viewers learn what attracts scientists to science, what it takes to become a scientist and what impact their work has on our world. The goal is to dispel stereotypes — to show scientists as people who lead interesting lives and are approachable and accessible, and to provide role models for women and minorities notably underrepresented in science. This video can be used in biology classes, in school career centers, or to prepare for visiting a local laboratory. Scientists also can use it when meeting with students and members of the public.
Director
Valli T. McDougle, Executive Producer
Producer
The University of California, San Francisco
Publication Date
June 1996/1996
Bibliographic Information 19-minute video and Teacher's Guide
On Becoming a Scientist is a fast-paced "MTV-style" video showing a "day in the life" of three graduate students and a laboratory manager. These young scientists are followed as they work in their laboratories, take part in UCSF programs for public school children and the homeless, and socialize. Through interviews with scientists and students, viewers learn what attracts scientists to science, what it takes to become a scientist and what impact their work has on our world. The goal is to dispel stereotypes — to show scientists as people who lead interesting lives and are approachable and accessible, and to provide role models for women and minorities notably underrepresented in science. This video can be used in biology classes, in school career centers, or to prepare for visiting a local laboratory. Scientists also can use it when meeting with students and members of the public.
Director
Valli T. McDougle, Executive Producer
Producer
The University of California, San Francisco
Publication Date
June 1996/1996
Bibliographic Information 19-minute video and Teacher's Guide
This handbook offers a practical guide to the principles of quantitative analysis in biological experiments. The material is primarily aimed at working molecular biologists, but the scope and clarity of presentation make it equally suitable as an introduction for students. Topics covered range from the basics—such as measuring the concentrations of macromolecules—through considerations of binding constants and the kinetics of molecular interactions. The book ends with a thorough consideration of data analysis.
Author
James A. Goodrich
University of Colorado, Boulder
Publication Date
2007/2007
Bibliographic Information 182 pp., illus., appendices, index
The application of computational methods to DNA and protein science is a new and exciting development in biology. Bioinformatics: Sequence and Genome Analysis is a comprehensive introduction to this emerging field of study. The book has many unique and valuable features:
It is written for any biologist who wants to understand methods of sequence and structure analysis and how the necessary computer programs work
Sequence alignment, structure prediction, phylogenetic and gene prediction, database searching, and genome analysis are clearly explained and amply illustrated
Underlying algorithms and assumptions are clearly explained for the non-specialist
Examples are presented in simple numerical terms rather than complex formulas and notation
Theoretical underpinnings are linked to biological problems and their solutions
Extensive tables provide descriptions and Web sources for a broad range of publicly available software
An associated Website (www.BioinformaticsOnline.org), accessible free of charge by book purchasers, provides links to Internet sources referred to in the text, as well as problem sets for classroom use, and other useful material not included in the text.
Based on the author's extensive experience as a molecular geneticist and bioinformaticist at the University of Arizona, this is a uniquely educational book, ideal as a laboratory reference for investigators and also as teaching reference for graduate and undergraduate students studying this fast-changing discipline.
The application of computational methods to DNA and protein science is a new and exciting development in biology. Bioinformatics: Sequence and Genome Analysis is a comprehensive introduction to this emerging field of study. The book has many unique and valuable features:
It is written for any biologist who wants to understand methods of sequence and structure analysis and how the necessary computer programs work
Sequence alignment, structure prediction, phylogenetic and gene prediction, database searching, and genome analysis are clearly explained and amply illustrated
Underlying algorithms and assumptions are clearly explained for the non-specialist
Examples are presented in simple numerical terms rather than complex formulas and notation
Theoretical underpinnings are linked to biological problems and their solutions
Extensive tables provide descriptions and Web sources for a broad range of publicly available software
An associated Website (www.BioinformaticsOnline.org), accessible free of charge by book purchasers, provides links to Internet sources referred to in the text, as well as problem sets for classroom use, and other useful material not included in the text.
Based on the author's extensive experience as a molecular geneticist and bioinformaticist at the University of Arizona, this is a uniquely educational book, ideal as a laboratory reference for investigators and also as teaching reference for graduate and undergraduate students studying this fast-changing discipline.
As more species’ genomes are sequenced, computational analysis of these data has become increasingly important. The second, entirely updated edition of this widely praised textbook provides a comprehensive and critical examination of the computational methods needed for analyzing DNA, RNA, and protein data, as well as genomes. The book has been rewritten to make it more accessible to a wider audience, including advanced undergraduate and graduate students. New features include chapter guides and explanatory information panels and glossary terms. New chapters in this second edition cover statistical analysis of sequence alignments, computer programming for bioinformatics, and data management and mining. Practically oriented problems at the ends of chapters enhance the value of the book as a teaching resource. The book also serves as an essential reference for professionals in molecular biology, pharmaceutical, and genome laboratories.
As more species’ genomes are sequenced, computational analysis of these data has become increasingly important. The second, entirely updated edition of this widely praised textbook provides a comprehensive and critical examination of the computational methods needed for analyzing DNA, RNA, and protein data, as well as genomes. The book has been rewritten to make it more accessible to a wider audience, including advanced undergraduate and graduate students. New features include chapter guides and explanatory information panels and glossary terms. New chapters in this second edition cover statistical analysis of sequence alignments, computer programming for bioinformatics, and data management and mining. Practically oriented problems at the ends of chapters enhance the value of the book as a teaching resource. The book also serves as an essential reference for professionals in molecular biology, pharmaceutical, and genome laboratories.
Author
David Mount
University of Arizona, Tucson
Publication Date
2004/2004
Bibliographic Information 665 pp., illus., appendices, index
Dioxin and its congeners have aroused considerable environmental concern in recent years. Their concentration levels and exposures are usually very small but generally persistent and there is still great debate about the effects of these compounds on humans and in ecosystems. New research and extensive discussion in the scientific community in the late 1980's shed new light on the chemistry, toxicology, and biology of dioxin. It seemed appropriate, therefore, for scientists engaged in dioxin research and officials from regulatory agencies in the US and Europe to gather at the Banbury Center of Cold Spring Harbor Laboratory to re-examine the assessment of dioxin's medical and environmental effects in the light of these new data.
They met with three aims in mind:
to review current knowledge of the low-dose chronic effects and mechanisms of action of dioxin;
to determine whether or not this knowledge provided a credible basis for assessing human risk from exposure to low dose levels;
to establish what problems would need resolution before an accord could be reached on the human risk of dioxin and related substances.
The proceedings of this important meeting are available in this monograph, which contains the speakers' presentations. The topicality of the meeting was confirmed by the April, 1991 decision of the Environmental Protection Agency to reevaluate the risks of exposure to dioxin "in the light of scientific advances that provide additional understanding of those risks."
This important volume should be read by scientists in all disciplines who are interested in dioxin research, environmental and public health officials, and representatives of industries in which dioxin release is of concern.
Editor
Michael A. Gallo
University of Medicine and Dentistry of New and Robert Wood Johnson Medical School
Publication Date
JANUARY 1991/1991
Bibliographic Information 501 pp., illus., indexes
McArdle Laboratory for Cancer Research,University of Wisconsin, Madison
Publication Date
1984/1984
Bibliographic Information 500 pp., illus., indexes
Set Info
Topics
Chemistry and General Pathology; Receptor Binding; Enzyme Induction;Biochemical Changes in Liver; Lipid Metabolism and Wasting Disease;Skin and In Vitro Responses; Immunological Mechanisms; Epidemiology
Living cells have evolved many ways of coping with metabolic events and environmental influences that damage DNA. These mechanisms, and the frequent progression to cancer that results when they go awry, are reviewed in this volume by authors from over sixty of the world’s leading laboratories. The topics discussed include DNA repair, mutagenesis and other damage-tolerance functions, checkpoint control, apoptosis, and adaptation. They draw from studies on human and yeast cells. Current, but with a valuable historical perspective, this volume has the depth and lasting value typical of this most prestigious series and is essential reading for investigators of DNA replication, cell cycle control, and tumorigenesis.
Publication Date
May 2001/2000
Bibliographic Information 613 pp., illus., color plates, appendices, indexes
Living cells have evolved many ways of coping with metabolic events and environmental influences that damage DNA. These mechanisms, and the frequent progression to cancer that results when they go awry, are reviewed in this volume by authors from over sixty of the world’s leading laboratories. The topics discussed include DNA repair, mutagenesis and other damage-tolerance functions, checkpoint control, apoptosis, and adaptation. They draw from studies on human and yeast cells. Current, but with a valuable historical perspective, this volume has the depth and lasting value typical of this most prestigious series and is essential reading for investigators of DNA replication, cell cycle control, and tumorigenesis.
Publication Date
May 2001/2000
Bibliographic Information 613 pp., illus., color plates, appendices, indexes
The Biological Revolution: 100 Years of Science at Cold Spring Harbor, a 30-minute video documentary providing the nonscientist with a cogent explanation of the origins of the DNA revolution that is now sweeping the country, is invaluable teaching material for junior and senior high school classes.
Using Cold Spring Harbor Laboratory as a case study, the video shows the parallel development of American science and society, while providing a first-hand account of the development of modern genetics. Through events that have occurred at Cold Spring Harbor, the video documents the quest to understand the nature of the genetic code, beginning with Darwin's theory of evolution and culminating with powerful techniques to precisely manipulate the DNA molecule.
Laboratory Director James Watson, who shared the Nobel Prize for elucidating the double-helix structure of DNA, serves as on-site host. He and four other Nobel laureates-Alfred Hershey, Salvador Luria, Barbara McClintock, and Walter Gilbert-lend their own insights on the genetic revolution they helped launch.
Viewers are brought up-to-the-minute on how recombinant DNA techniques are being employed in the wars against cancer and world hunger. Included is stunning footage which shows cancer-causing proteins being injected directly into living cells using a needle with a tip 1/100th the diameter of a human hair.
The Biological Revolution: 100 Years of Science at Cold Spring Harbor, a 30-minute video documentary providing the nonscientist with a cogent explanation of the origins of the DNA revolution that is now sweeping the country, is invaluable teaching material for junior and senior high school classes.
Using Cold Spring Harbor Laboratory as a case study, the video shows the parallel development of American science and society, while providing a first-hand account of the development of modern genetics. Through events that have occurred at Cold Spring Harbor, the video documents the quest to understand the nature of the genetic code, beginning with Darwin's theory of evolution and culminating with powerful techniques to precisely manipulate the DNA molecule.
Laboratory Director James Watson, who shared the Nobel Prize for elucidating the double-helix structure of DNA, serves as on-site host. He and four other Nobel laureates-Alfred Hershey, Salvador Luria, Barbara McClintock, and Walter Gilbert-lend their own insights on the genetic revolution they helped launch.
Viewers are brought up-to-the-minute on how recombinant DNA techniques are being employed in the wars against cancer and world hunger. Included is stunning footage which shows cancer-causing proteins being injected directly into living cells using a needle with a tip 1/100th the diameter of a human hair.
Biology of Drosophila was first published by John Wiley and Sons in 1950. Until its appearance, no central, synthesized source of biological data on Drosophila melanogaster was available, despite the fly's importance to science for three decades. Ten years in the making, it was an immediate success and remained in print for two decades. However, original copies are now very hard to find. This facsimile edition makes available to the fly community once again its most enduring work of reference.
Editor
M. Demerec
Publication Date
2008/2008
Bibliographic Information 632 pp., illus., indexes
Biology of Drosophila was first published by John Wiley and Sons in 1950. Until its appearance, no central, synthesized source of biological data on Drosophila melanogaster was available, despite the fly's importance to science for three decades. Ten years in the making, it was an immediate success and remained in print for two decades. However, original copies are now very hard to find. This facsimile edition makes available to the fly community once again its most enduring work of reference.
Editor
M. Demerec
Publication Date
November 1994/1994
Bibliographic Information 632 pp., illus., indexes
The heat shock proteins and molecular chaperones form large gene families and are active in every cellular compartment, regulating protein folding, translocation and complex assembly. A sequel to the 1990 volume, Stress Proteins in Biology and Medicine, this monograph updates progress and introduces new fields of investigation. Particular emphasis is given to the biochemical properties of hs proteins and chaperones in protein biogenesis, the role of hs proteins in thermotolerance and cytoprotection, transcriptional regulation of hs genes, and the function of the hs response in immunology and disease.
This is a thoughtful and comprehensive account of a field of investigation that has been developing at a breathtaking pace. The book is a vital resource for investigators in cell biology, protein biochemistry and molecular genetics, and those with interests in immunity, ischemic disease and aging.
Mammalian germ-cell mutation research is advancing rapidly. Views of barriers and targets mediating the induction of mutations in the germ line are changing, and new molecular and cytological methods are converging with traditional experimentation in the field to detect mutations and assess their nature, expression, and transmission.
This book ties together research findings in reproductive biology, molecular and cellular mechanisms of mutagenesis, mutation expression, and risk assessment. It provides an unusually broad perspective of the unique biological aspects of germ-cell mutations, with emphasis on male systems. Germ-line properties affecting the induction and recovery of such mutations, and variables affecting their rate and nature, are stressed. Related topics include aberrant chromosome structure and behavior, nonmutational genetic effects on early development, and the utilization of DNA techniques to detect germ-line mutations. Discussions in these areas lead up to the final chapter on genetic risk estimation which focuses on the central problems and issues facing those responsible for assessing risks associated with germ-cell mutations.
The central question in neurobiology is: How does the brain work? At the beginning of the 1980s, armed with the new techniques of recombinant DNA and monoclonal antibodies, molecular biologists began to approach some aspects of this question without being intimidated by the formidable facts of electrophysiology or neuroanatomy. In the past decade these first steps toward the last frontier of biology have quickened and lengthened. Talented investigators have brought to the field expertise and imagination derived from other branches of biology and computer science. These have been applied to the study of nerve cell growth and differentiation, signal reception and transduction, and networked cellular interactions in such fundamental processes as vision, olfaction, learning, memory, and motor control. Over 100 outstanding scientists were invited to Cold Spring Harbor's 55th annual Symposium to present their latest ideas and data.
In May 1991, their papers were published in this volume of the most prestigious book series in biology. Symposium LV presents current neurobiology in all its dramatic diversity and is an unrivaled resource for workers in this field.
Publication Date
JANUARY 1991/1990
Bibliographic Information 1,082 pp., illus., color plates, indexes
The central question in neurobiology is: How does the brain work? At the beginning of the 1980s, armed with the new techniques of recombinant DNA and monoclonal antibodies, molecular biologists began to approach some aspects of this question without being intimidated by the formidable facts of electrophysiology or neuroanatomy. In the past decade these first steps toward the last frontier of biology have quickened and lengthened. Talented investigators have brought to the field expertise and imagination derived from other branches of biology and computer science. These have been applied to the study of nerve cell growth and differentiation, signal reception and transduction, and networked cellular interactions in such fundamental processes as vision, olfaction, learning, memory, and motor control. Over 100 outstanding scientists were invited to Cold Spring Harbor's 55th annual Symposium to present their latest ideas and data.
In May 1991, their papers were published in this volume of the most prestigious book series in biology. Symposium LV presents current neurobiology in all its dramatic diversity and is an unrivaled resource for workers in this field.
Publication Date
JANUARY 1991/1990
Bibliographic Information 1,082 pp., illus., color plates, indexes
What happens when you read? Your eyes see, your mind thinks, your fingers turn the pages. This is all organized by your brain. What does your brain look like? What is it made of? Your brain sends and receives messages, makes and stores memories, controls feelings and behavior and much more. Learn how to keep your brain fit and busy and join in the Mega Memory Experiment.
Brainbox Memory Game
Steven Rose would like to find out how well people remember things as they get older. If you want to join in his experiment you can try the memory game from the book by clicking here:
Steven Rose directs the Brain and Behavior Research Group at the Open University, where he is Professor of Biology. He researches the biological mechanisms of learning and memory. His most recent books for adults include The Making of Memory (winner of the 1993 Rhone-Poulenc Science Book prize); Molecules and Minds, and Not in Our Genes. Brainbox is co-written with Alexander Lichtenfels who goes to school in Leeds. Alexander founded, edited and wrote for a magazine, Red Alert, sold in aid of Greenpeace.
Author
Steven Rose
Contributor
Mic Rolph
Editor
Fran Balkwill
Publication Date
January 1997/1997
Bibliographic Information 32 fully illustrated 4-color pages
Derived from the acclaimed online “WormAtlas,” C. elegans Atlas is a large-format, full-color atlas of the hermaphroditic form of the model organism C. elegans, known affectionately as “the worm” by workers in the field. Prepared by the editors of the WormAtlas Consortium, David H. Hall and Zeynep F. Altun, this book combines explanatory text with copious, labeled, color illustrations and electron micrographs of the major body systems of C. elegans. Also included are electron microscopy cross sections of the worm. This laboratory reference is essential for the working worm biologist, at the bench and at the microscope, and provides a superb companion to the C. elegans II monograph. It is also a valuable tool for investigators in the fields of developmental biology, neurobiology, reproductive biology, gene expression, and molecular biology.
Author
David H. Hall
Albert Einstein College of Medicine, New York
Publication Date
2008/2008
Bibliographic Information 348 pp., illus., appendix, index
Derived from the acclaimed online “WormAtlas,” C. elegans Atlas is a large-format, full-color atlas of the hermaphroditic form of the model organism C. elegans, known affectionately as “the worm” by workers in the field. Prepared by the editors of the WormAtlas Consortium, David H. Hall and Zeynep F. Altun, this book combines explanatory text with copious, labeled, color illustrations and electron micrographs of the major body systems of C. elegans. Also included are electron microscopy cross sections of the worm. This laboratory reference is essential for the working worm biologist, at the bench and at the microscope, and provides a superb companion to the C. elegans II monograph. It is also a valuable tool for investigators in the fields of developmental biology, neurobiology, reproductive biology, gene expression, and molecular biology.
Author
David H. Hall
Albert Einstein College of Medicine, New York
Publication Date
2008/2008
Bibliographic Information 348 pp., illus., appendix, index
Studies of the cells and genes of the nematode C. elegans have become a cornerstone of current biology. A classic 1988 Cold Spring Harbor monograph, The Nematode Caenorhabditis elegans, described the basic genetics, anatomy and development of the organism. Now, in that authoritative tradition, comes C. elegans II -- not a second edition but a book that breaks new ground and defines the current status of the field, providing a detailed molecular explanation of how development is regulated and the nervous system specifies varied aspects of behavior. This volume is a must for any investigator doing worm studies but it has been written and rigorously edited to illuminate for a wider community of investigators in cell and molecular biology who should know how new knowledge of C. elegans relates to their own specialty.
Editor
Donald L. Riddle
University of Missouri, Columbia
Publication Date
February 1997/1997
Bibliographic Information 1222 pp., illus., color plates, index
As the world of biotechnology has grown in leaps and bounds, so too have the career opportunities. But the choices can be daunting. What types of jobs are available? How do you get your foot in the door? What will your job entail if you become a “Preclinical Project Manager” or a “Process Scientist”? What’s the difference between biotech and pharma?
Career Opportunities in Biotechnology and Drug Development provides a comprehensive and systematic overview of careers in the life science industry, with all their ups and downs. The author, Toby Freedman, Ph.D., has conducted interviews with hundreds of key players in the industry, who provide first–hand explanations of their day–to–day roles and responsibilities, and offer key insights into how they landed those jobs in the first place. Careers in everything from discovery research to venture capital are covered in detail.
Each chapter includes valuable sections on preparing yourself for a prospective career: educational requirements and personality characteristics needed; recommendations of books, magazines, and Web site resources; and issues to consider regarding salary and compensation. The book also includes interviewing and job searching tips, as well as suggestions on writing a resume specifically for industry.
Career Opportunities in Biotechnology and Drug Development is an essential guide for science graduates and medical, business, legal, high–tech or engineering professionals. With discussions of job security, future trends, and potential career paths, even those already working in industry will find helpful information on how to take advantage of opportunities available within their own companies and elsewhere. This book will help you make wiser and more informed decisions about what role you would like to play in the biotechnology and drug development industry.
This monograph, written by experts in the field, is devoted to the molecular analysis of addiction pathways in the brain. It provides an intensive overview of the fundamentals, state–of–the–art advances, and major gaps in the cell and molecular biology of drug addiction within the broader context of neuroscience. Addiction research is a branch of neuroscience and psychology. The emphasis in this book is on hard science and the market for it will be found among research investigators and grad students within the field of neuroscience. The research presented is not only applicable to the study of drug abuse and addiction, but has clear implications for clarifying mechanisms of learning and memory, neuroadaptation, perception, volitional behavior, motivation, reward, and other disciplines of neuroscience.
Editor
Bertha K. Madras
Harvard Medical School
Publication Date
2006/2006
Bibliographic Information 465 pp., illus., appendices, index
All cells make orderly transitions between states of division and rest. The events of this cycle are extraordinary to look at. Chromosomes condense, the nucleus breaks down, a complex spindle is built, and daughter cells are born -- all with a precision and control that beg for explanation and understanding.
In the past three years, some of the complex biochemistry underlying these events has become known. Experiments in yeast, frog, and human cells have suggested that the enzyme pathway involved may be much the same in all eukaryotes. With a generally accepted model in place, several master protein components and their genes are identifiable. The details of their functions and interactions are now a major preoccupation in cell biology.
The 56th annual Cold Spring Harbor Symposium was acclaimed as a key event for progress in this field. Over 90 leading investigators presented data on DNA replication, mitosis, cell cycle controls, checkpoints affecting cycling, and transcriptional control. Included in the Symposium was the first evidence that oncogene and tumor suppressor gene products and protein components of signal transduction pathways directly interact with enzymes that control the cell division cycle.
This volume of collected papers from the Symposium is an unrivaled resource for students and researchers studying cell and molecular biology.
Publication Date
JANUARY 1992/1991
Bibliographic Information 782 pp., illus., indexes
The biochemical events underlying cell growth and passage through the division cycle are closely related, yet tend to be studied in different ways: growth control and oncogenesis in cultures of mammalian cells in vitro and passage through the cell cycle by studies in yeast, sea urchins, and frogs. As a result of rapid advances in the molecular characterization of oncogenes, growth factors, and cell cycle genes, a coherent understanding of how the events of the cell cycle are controlled is emerging. This survey of this evolving topic will benefit all who are interested in the cell and molecular biology of the cell cycle and cell growth.
Recent breakthroughs in the field of cell growth, particularly in the control of cell size, are reviewed by experts in the three major divisions of the field: growth of individual cells, growth of organs, and regulation of cell growth in the contexts of development and cell division. This book is an introductory overview of the field and should be adaptable as a textbook.
Editor
Michael N. Hall
Biozentrum, University of Basel
Publication Date
2004/2004
Bibliographic Information 652 pp., illus., appendices, index
So much has been learned about the surface of cells that it can almost be counted as an organelle. The surface has been revealed as a complex assembly of proteins, highly mobile in time and space, with overlapping activities and shared structural motifs. The locations of their genes and the untangling of their functions are major challenges being tackled by laboratories worldwide.
The 57th annual Cold Spring Harbor Symposium brought together over 80 leading investigators to discuss data and ideas on receptor-ligand interactions, membrane organization, signal transduction, peptide transport, cell adhesion, and the regulation of developmental processes. Studies in molecular and cell biology, embryology, neurobiology, and immunology were presented.
This volume of collected papers from the Symposium, like its predecessors in this most prestigious series, provides a wide-ranging, eclectic review of a topic central to the understanding of cell structure and function.
Publication Date
JANUARY 1993/1992
Bibliographic Information 707 pp., illus., color plates, indexes
So much has been learned about the surface of cells that it can almost be counted as an organelle. The surface has been revealed as a complex assembly of proteins, highly mobile in time and space, with overlapping activities and shared structural motifs. The locations of their genes and the untangling of their functions are major challenges being tackled by laboratories worldwide.
The 57th annual Cold Spring Harbor Symposium brought together over 80 leading investigators to discuss data and ideas on receptor-ligand interactions, membrane organization, signal transduction, peptide transport, cell adhesion, and the regulation of developmental processes. Studies in molecular and cell biology, embryology, neurobiology, and immunology were presented.
This volume of collected papers from the Symposium, like its predecessors in this most prestigious series, provides a wide-ranging, eclectic review of a topic central to the understanding of cell structure and function.
Publication Date
JANUARY 1993/1992
Bibliographic Information 707 pp., illus., color plates, indexes
After reading this fully illustrated account of immune responses and other anti-microbial reactions, a child will view illness quite differently. He/she will have a better understanding about what actually happens up one's nose, down one's throat, and anywhere else that has been invaded by germs. (Ages 7-13)
Author
Fran Balkwill
Contributor
Mic Rolph
Publication Date
JANUARY 1991/1990
Bibliographic Information 32 fully illustrated 4-color pages
This is a book about the various types of cells that make up the human body. By using lively and expressive language, and by portraying the different cells with colorful and imaginative drawings, the author and artist teach the reader how an individual person is created from just one cell. (Ages 5-8)
Author
Fran Balkwill
Contributor
Mic Rolph
Publication Date
JANUARY 1991/1990
Bibliographic Information 32 fully illustrated 4-color pages
This book is the first scholarly history of research into the genetics of body cells, from its origins in the 19th century to the present day. Henry Harris, a well-known writer and a distinguished investigator in cell biology and cancer genetics, brings an unusually informed perspective to the technical aspects of his subject. He has written a book to be enjoyed not just by professional historians of science, but by working scientists in genetics, cell biology, and cancer research, from the graduate student level upwards. Its readers will derive a richer understanding of how and why the cells of the body are studied in the way that they are today.
Author
Henry Harris
University of Oxford
Publication Date
December 1997/1995
Bibliographic Information 263 pp., illus., indexes
Having identified a gene product, how do you determine what it does? The answer lies in Cells, a new manual designed to do for studies of cell biology what Cold Spring Harbor's Molecular Cloning has done for molecular biology.
Sets the standard for techniques of proven bench reliability needed by all biomedical scientists studying cellular structure and function.
Delivers consistent, precisely crafted step-by-step protocols in an accessible format, with essential background details and in-depth advice on pitfalls and problem solving.
Created by three distinguished cell biologist/educators, from the contributions of over 180 leading cell biologists.
Complete with more than 300 expertly selected and superbly reproduced illustrations, over 70 in color.
Volume 1: Culture and Biochemical Analysis of Cells
Volume 2: Light Microscopy and Cell Structure
Volume 3: Subcellular Localization of Genes and Their Products
Editor
David L. Spector
Cold Spring Harbor Laboratory
Publication Date
December 1997/1998
Bibliographic Information 2136 pp., illus., color plates, appendices, index
141 striking, specially created color illustrations from Cells: A Laboratory Manual are now available as a set of slides, with a 26-page booklet of explanations.
An expertly crafted series of 35-mm slides complete with diagrams to show the basic principles and color examples of microscopic applications.
Perfect for developing course lectures on:
Principles of Microscopy
Imaging Alternatives using the Light Microscope
Basic Approaches using the Electron Microscope
Editor
David L. Spector
Cold Spring Harbor Laboratory
Publication Date
October 1998/1999
Bibliographic Information 141 slides and booklet of explanations
Airborne fibers have long been recognized as a major health hazard in certain industries and exposure to fibers in the general environment may also cause adverse effects such as cancer, in particular, mesothelioma. Asbestos is the best-known fiber carcinogen and, in the construction industry, is being widely replaced by substitute materials. Yet to perform properly, these materials must physically resemble asbestos. Does this mean that they too are hazardous?
In this volume, invited experts in cell and tumor biology review the processes of cell division and the influence of fibers upon them. The biology of tumors and other diseases of the lung is discussed in some detail and in several papers the molecular basis of malignant transformation in mesothelioma cells is considered. These contributions combine to give the book a unique, scientific slant on an important environmental issue. It will interest research scientists investigating carcinogenesis and the biology of cell division, thoracic physicians, public health specialists, and those concerned with environmental control policy.
Accurate transmission of genetic information in cells is ensured by DNA repair, delays in replication until repairs are complete, and by cell death. Work in yeast and subsequently in mammalian cells has shown that monitoring the cell cycle generates signals which halt it at specific "checkpoints". Failures in these processes can release uncontrolled cell division. This volume examines the mechanisms of checkpoint control, the cellular activities they influence, and their importance in understanding the fundamentals of the neoplastic process. An illuminating book for both researchers and clinicians in oncology.
Editor
M.B. Kastan
Johns Hopkins Oncology Center
Publication Date
/1997
Bibliographic Information 363 pp., illus., color plates, index
Based on presentations by world-renowned investigators at the 72nd annual Cold Spring Harbor Symposium on Quantitative Biology, this volume reviews the latest advances in biological clocks and rhythms. Topics include genetic and cellular studies aimed at characterizing circadian mechanisms; systems approaches to understanding physiological, endocrine, and neural networks; and models used for studying mental and physical cycles. A variety of normal and abnormal chronobiological patterns are discussed, including sleep, aging, migration, hibernation, seasonality, depression, and arrhythmias.
Editor
Bruce Stillman
Cold Spring Harbor Laboratory
Publication Date
2007/2007
Bibliographic Information 674 pp., illus., indexes (Hardcover edition includes online access; call for information and registration)
Based on presentations by world-renowned investigators at the 72nd annual Cold Spring Harbor Symposium on Quantitative Biology, this volume reviews the latest advances in biological clocks and rhythms. Topics include genetic and cellular studies aimed at characterizing circadian mechanisms; systems approaches to understanding physiological, endocrine, and neural networks; and models used for studying mental and physical cycles. A variety of normal and abnormal chronobiological patterns are discussed, including sleep, aging, migration, hibernation, seasonality, depression, and arrhythmias.
Editor
Bruce Stillman
Cold Spring Harbor Laboratory
Publication Date
2007/2007
Bibliographic Information 674 pp., illus., indexes (Paperback edition does not include online access)
A concise, readable account of the principles underlying embryonic and appendage development in animals and plants, illustrated with colorful artwork and interviews with prominent investigators. Highly recommended for use in undergraduate and graduate courses.
Author
Ethan Bier
University of California, San Diego
Contributor
Cliff Tabin
Harvard Medical School
Publication Date
August 2000/2000
Bibliographic Information 252 pp., illus., color plates, index, glossary, bibliography
A concise, readable account of the principles underlying embryonic and appendage development in animals and plants, illustrated with colorful artwork and interviews with prominent investigators. Highly recommended for use in undergraduate and graduate courses.
Author
Ethan Bier
University of California, San Diego
Contributor
Cliff Tabin
Harvard Medical School
Publication Date
August 2000/2000
Bibliographic Information 252 pp., illus., color plates, index, glossary, bibliography
This book, which includes the first fourteen chapters of the acclaimed monograph DNA Replication in Eukaryotic Cells, is a broad account of the basic principles of DNA replication and related functions such as DNA repair and protein phosphorylation. It also includes a newly commissioned chapter in which the editor, Mel DePamphilis, provides a review of the most recent advances in understanding the complex gene and protein interactions that underpin this essential cellular function.
DNA Replication in Eukaryotic Cells has been acclaimed as “...an absolute must for anyone working in the DNA replication field” (Trends in Cell Biology), “...highly significant addition to the replication literature” (Trends in Biochemical Sciences) and “...an invaluable addition for libraries...the first place to stop for anyone wishing to enter the eukaryotic replication field” (The Quarterly Review of Biology). This abridged edition is ideal for graduate students or established investigators who need a complete and timely introduction to this rapidly advancing field of research.
Editor
Melvin L. DePamphilis
National Institutes of Health
Publication Date
November 1998/1999
Bibliographic Information 520 pp., illus., color plates, index
An English translation of Boveri’s famous monograph which was first published in Germany in 1914.
Written almost a hundred years ago, Theodor Boveri’s Zur Frage der Entstehung maligner Tumoren has had a momentous impact on cancer research. In it he argues that malignancy arises as a consequence of chromosomal abnormalities and that multiplication is an inherent property of cells. With astonishing prescience, Boveri predicts in this monograph the existence of tumor suppressor mechanisms and is perhaps the first to suggest that hereditary factors (genes) are linearly arranged along chromosomes. This new translation by Sir Henry Harris, Regius Professor of Medicine Emeritus at Oxford University and former Editor-in-Chief of Journal of Cell Science, includes extensive annotations in which he discusses the relevance of Boveri’s views today. It is essential reading for all cancer researchers, as well as those interested in the history of cytogenetics and cell biology.
Based on presentations by world-renowned investigators at the 73rd annual Cold Spring Harbor Symposium on Quantitative Biology, this volume reviews the latest advances in research on the control and regulation of stem cells. The topics covered include nuclear reprogramming, regulation of stem cell self-renewal and differentiation, the stem cell niche, and signaling and gene regulation in stem cells. Studies of embryonic stem cells and adult stem cells are covered, along with research shedding light on the roles of these cells in regeneration and cancer.
Editor
Terri Grodzicker
Cold Spring Harbor Laboratory
Publication Date
June 2009/2008
Bibliographic Information 650 pp. (approx.), illus., indexes (Paperback edition does not include online access)
Based on presentations by world-renowned investigators at the 73rd annual Cold Spring Harbor Symposium on Quantitative Biology, this volume reviews the latest advances in research on the control and regulation of stem cells. The topics covered include nuclear reprogramming, regulation of stem cell self-renewal and differentiation, the stem cell niche, and signaling and gene regulation in stem cells. Studies of embryonic stem cells and adult stem cells are covered, along with research shedding light on the roles of these cells in regeneration and cancer.
Editor
Terri Grodzicker
Cold Spring Harbor Laboratory
Publication Date
June 2009/2008
Bibliographic Information 650 pp. (approx.), illus., indexes (Hardcover edition includes online access; call for information and registration)
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Producer
Executive Producer, Rochelle Easton Esposito
The University of Chicago
Publication Date
2002/2002
Bibliographic Information Set of 5 DVD discs, NTSC version, region unencoded
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Producer
Executive Producer, Rochelle Easton Esposito
The University of Chicago
Publication Date
2005/2005
Bibliographic Information Set of 5 DVD discs, NTSC version, region unencoded
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Bibliographic Information DVD disc (NTSC version, region unencoded), 108 minutes in length (Part 1: Physics and Phage - 69 minutes; Part 2: Behavioral Genetics - 39 minutes)
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each Volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Producer
the Following
Publication Date
2002/2002
Bibliographic Information Set of 5 DVD discs, NTSC version, region unencoded
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each Volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each Volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each Volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each Volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
Conversations in Genetics is a collection of videotaped conversations with geneticists who have made major contributions to the conceptual foundations of modern genetics. These presentations reflect the thoughts and feelings of accomplished researchers as they recall their research achievements and describe the paths they took during various phases of their lives. They provide a rich resource for anyone interested in the history of genetics and the evolution of scientific ideas.
Each Volume of Conversations is comprised of five DVDs. They can be purchased individually or as a set of five.
Click links below to see a sample video interview from this DVD.
In this brief, readable, and revealing book, one of the pioneers of the now rapidly evolving field of DNA repair traces the history of the discovery of the more important mechanisms by which cells respond to DNA damage. Errol Friedberg has written an enjoyable and informative introduction to the study of DNA mutagenesis and re-pair that will interest students at an advanced undergraduate or graduate student level as well as investigators in fields as diverse as oncogenesis, cell cycle regulation, transcription and DNA replication.
Author
Errol C. Friedberg
The University of Texas SouthwesternMedical Center, Dallas
Crystals of macromolecules have become keystones of molecular biology, bridging recombinant DNA and X-ray diffraction analysis. Protein, nucleic acid, and virus crystal growth are now possible, raising hopes that virtually all macromolecules might be studied in this way. This extensively illustrated book, by a master practitioner, provides a biochemical context in which crystal growth can be pursued and instructs readers in practical aspects of the technology, laying out effective strategies for success. The underlying physical and chemical principles are presented in approachable form and the most recent advances are described, those that have succeeded in transforming the growth of macromolecular crystals from a "black art" to a firmly founded science.
This readable volume is highly recommended for every investigator in biomedicine whose studies may require a shift in focus from gene to protein product, as well as chemists and physicists interested in the function of biologically active macromolecules.
Author
Alexander McPherson
University of California, Irvine
Publication Date
December 1998/1999
Bibliographic Information 586 pp., illus., appendix, index
The double helix, the genetic code, jumping genes, the PCR technique, the human genome project, RNA interference ... These and hundreds of other important advances in biology were announced, debated, and distilled at the Cold Spring Harbor Symposia. These meetings, held each year on tranquil grounds of one of the world's leading research institutes, have been notable events in biomedical research since 1933.
Now this essential archive, dating from 1933 to 2003, is going online. Learn more about the CSH Symposia Online Archive by visiting the Symposia website.
The main purpose of this Banbury Conference was to document the state of the art of cyto-skeletal proteins as markers of differentiation and cell type. Proteins discussed included intermediate filaments, keratins, actins, MAPs, Tau proteins, tropomyosins, myosins, spectrins, villin, synaptophysin, desmosomal proteins, and cell adhesion molecules. Emphasis was placed not only on the cell and molecular biology of these molecules, but also on how far antibodies could be used to distinguish different isoforms present in different cell types in normal and fetal tissues. The meeting resulted in a particularly lively discussion, reflected in the papers, of the advantages and inherent limitations of using such molecules as aids in determining tumor types.
Editor
M. Osborn
Max-Planck-Institute for BiophysicalChemistry, Göttingen
In 1911, the influential geneticist Charles Davenport published Heredity in Relation to Eugenics, advancing his ideas of how genetics would improve society in the 20th century. It became a college textbook and a foundation for the widespread eugenics movement in the United States. Nearly 100 years later, many of the issues raised by Davenport are again being debated, in different guises. In this new volume, prominent academics discuss themes from Davenport’s book—human genetic variation, mental illness, nature vs. nurture, human evolution—in a contemporary context. Davenport’s original book is reprinted along with the essays. This book will be useful to historians of science as well as those interested in the social implications of human genetics research—past, present, and future.
This volume summarizes research on the use of molecular markers for gene mapping in plants. It is the first synthesis of progress in an area that has important consequences for both basic and applied research. The rapid development in this field has produced complete molecular maps in at least eight species and partial maps in a number of others. The density of these maps permits the genetic analysis of quantitative traits with an accuracy not previously possible. The ability to map quantitative trait loci accurately is likely to have profound effects on the nature of quantitative genetics.
Editor
Timothy Helentjaris
Native Plants, Inc.
Publication Date
JANUARY 1989/1989
Bibliographic Information 165 pp., illus.
Set Info
Topics
Topics addressed include practical considerations in the constructionof molecular and physical maps, the use of cytogenetic tools torelate physical and genetic maps, the molecular nature of geneticpolymorphism and its relationship to speciation, statistical methodsfor mapping quantitative trait loci, the nature of quantitative traitloci and prospects for cloning these factors, the efficacy ofmolecular markers in marker-based selection and backcrossintrogression, and the development of new technologies for measuringpolymorphism.
The idea of human gene therapy was accepted by the medical community and society at large long before believable clinical benefits began to emerge. In this book, some of the field’s most distinguished contributors chronicle the evolution of this momentous direction for medicine, illustrating how imaginative concepts shaped the development of technologies and brought the daring new idea to its current position of imminent practical success. This is a book designed to endure as clinical advances accumulate, a clear-eyed work of reference that will anchor the further development of this revolution in therapy. It is an essential addition to libraries of clinical medicine, biotechnology, and public policy, and a resource that no laboratory investigator with an interest in the biology of gene transfer should be without.
Editor
Theodore Friedmann
University of California, San Diego
Publication Date
December 1998/1999
Bibliographic Information 729 pp., illus., color plates, index
Discovering Genomics, Proteomics, & Bioinformatics is the first text to combine real–world data and web activities with a hands–on approach to learning the fundamentals of genomic analysis. Written more like a workbook than a text, Discovering Genomics, Proteomics, & Bioinformatics has been thoroughly revised and updated to incorporate the latest scientific findings on genetic defects, disease–causing organisms, and other fast–breaking developments in genomics relevant to our lives.
With Discovering Genomics, Proteomics, & Bioinformatics you will:
Gain real–life context for genomics concepts through case study chapters appearing throughout the text
Visualize 3–D DNA structures on the interactive companion website (www.GeneticsPlace.com), now with Jmol
Research and analyze real genomics data as you work your way through each chapter of the companion website (www.GeneticsPlace.com)
Discover genomics through updated and expanded Discovery Questions appearing throughout each chapter
Learn genomics through hands–on practice, including using databases to extract pertinent information
Get help when you need it through updated and expanded Math Minutes—brief tutorials that reveal the math behind the biology and illustrate how investigators apply math in solving specific biology problems
Also available: Instructor’s Manual (ISBN 0-8053-8218-6)—the printed version of the Instructor’s Manual provides answers to all the Discovery Questions. In addition to the written answers, the electronic version of the Instructor’s Manual (www.aw-bc.com/genomics) provides color figures to accompany some answers. For example, if students are asked to draw a graph or construct a circuit diagram, your electronic version will illustrate one possible answer. These illustrations, along with all illustrations on the web site, may be used for lecture presentation such as PowerPoint or web pages. Access to these answers is restricted to faculty who have adopted the textbook for course use. Students cannot access this resource. Contact your Benjamin Cumming sales representative for your free password.
Author
A. Malcolm Campbell
Davidson College
Publication Date
2007/2007
Bibliographic Information 447 pp., glossary, index
Discovering Genomics, Proteomics, and Bioinformatics is the first textbook which combines integrated web exercises with a problem–solving approach to train students in basic hands–on genomic analysis. The authors present global problems, then provide the tools of genomic analysis to help students dissect the answer, thus encouraging critical–thinking skills. Microarray images and all art and figures from the text are available on the free art CD–ROM. The Discovering Genomics, Proteomics, and Bioinformatics Companion Website www.geneticsplace.com helps students learn about cutting–edge research and visualize complex processes. This integrated learning package sets the standard for material in the exciting new field of genomics.
FEATURES:
First genomics textbook written for students that focuses on the process of doing genomic analysis and thinking from a genomics perspective.
Inquiry approach gives students hands–on practice and builds problem solving skills. Students learn to use databases and how to extract pertinent information.
Math Minutes supply brief tutorials that reveal the math behind the biology and illustrate how investigators apply math in solving specific biology problems.
Discovery Questions appear throughout each chapter and ask students to consider what is happening in a particular example or case study, thus engaging students in taking a genomic approach to real–world problems.
Case studies and real–world examples provide a context for understanding the experiments and results.
Integrated multimedia and web resources direct students to a full methods index, relevant web links, and animations that allow them to research and analyze real genomics data as they work their way through each chapter. Icons in the text alert students to these media tools.
This book is the syllabus for an innovative and imaginative course in molecular genetics given by the author at UCLA for the past seven years. Its unique features include:
A case-study approach, based on analysis of classic and recent papers and discussion of the lives of the principal investigators concerned
Introductory essays which review the key concepts in each course unit
Over 180 questions, with answers, which test factual knowledge derived from each unit
Over 140 problems, including scenarios from history, mythology, movies, and TV series, which test students' abilities to apply molecular genetic concepts. Solutions and strategies for the problems are provided in a companion workbook.
This novel, fully illustrated textbook offers teachers a ground-breaking but fully validated 1-semester syllabus for use by life science majors (microbiology, biochemistry, molecular biology, or biology) or pre-med students. It is thoroughly recommended for course adoption. Examination copies are available to teachers on application.
Author
Jeffrey H. Miller
University of California, Los Angeles
Publication Date
December 1995/1996
Bibliographic Information 696 pp., illus., indexes
This book has been produced using print on demand technology.
This workbook is a companion to Discovering Molecular Genetics: A Case Study Course with Problems and Scenarios. One of the unique features of the latter is:
Over 140 problems, including scenarios from history, mythology, movies, and TV series, which test students' abilities to apply molecular genetic concepts.
Solutions and strategies for these problems are provided in the companion workbook, which is also available separately.
Author
Jeffrey H. Miller
University of California, Los Angeles
Publication Date
June 1996/1996
Bibliographic Information 80 pp.
This book has been produced using print on demand technology.
Discovering Neurons is a collection of investigative laboratory experiments designed for the advanced undergraduate and the first or second year graduate student. In its three major sections, neurons are studied as:
individual cells, including topics such as intracellular recording, immunocytochemistry and HPLC
forming connections, with experiments on tract tracing, information coding and LTP, among others
as coordinators of behavior, through approaches such as pharmacology, lesioning and ethology.
The fourth section focuses on techniques applicable to several experiments in the collection, including electrophysiology recording, surgery, anesthetics and staining methods.
The experiments introduce students to the techniques used in today's leading neuroscience research labs. They have been thoroughly tested in the student laboratory and are written and designed to be easily integrated into existing neuroscience courses of any length or emphasis. This is a unique volume that is an important resource for all teachers of neuroscience.
Contributor
Barbara Beltz
Wellesley College
Editor
Carol Ann Paul
Wellesley College
Publication Date
October 1997/1997
Bibliographic Information 420 pp., illus., appendices, index
Discovering Neurons is a collection of investigative laboratory experiments designed for the advanced undergraduate and the first or second year graduate student. In its three major sections, neurons are studied as:
individual cells, including topics such as intracellular recording, immunocytochemistry and HPLC
forming connections, with experiments on tract tracing, information coding and LTP, among others
as coordinators of behavior, through approaches such as pharmacology, lesioning and ethology.
The fourth section focuses on techniques applicable to several experiments in the collection, including electrophysiology recording, surgery, anesthetics and staining methods.
The experiments introduce students to the techniques used in today's leading neuroscience research labs. They have been thoroughly tested in the student laboratory and are written and designed to be easily integrated into existing neuroscience courses of any length or emphasis. This is a unique volume that is an important resource for all teachers of neuroscience.
Contributor
Barbara Beltz
Wellesley College
Editor
Carol Ann Paul
Wellesley College
Publication Date
October 1997/1997
Bibliographic Information 420 pp., illus., appendices, index
The structure of DNA proposed by James Watson and Francis Crick in 1953 was one of the most important scientific discoveries of the twentieth century, transforming biology, giving medicine new impetus, and providing a foundation for the new industry of biotechnology. Jim Watson’s best-selling memoir of the events leading to this discovery, The Double Helix, has enthralled millions of readers since its publication in 1968. In this videotaped lecture, recorded live at Cold Spring Harbor Laboratory in November 1999, Dr. Watson retraces those events in a very personal reminiscence of the period and the people involved. This is an account of one of the great dramas of science, told from the intimate perspective of a participant who, aged 25 at the time of the discovery, has become one of the intellectual leaders of our time.
James D. Watson was Director of Cold Spring Harbor Laboratory, New York, from 1968 to 1993 and is now its President. He was the first Director of the National Center for Human Genome Research of the National Institutes of Health from 1989 to 1992.
Dr. Watson is a member of the National Academy of Sciences and Royal Society and has received many awards and honors, including the Presidential Medal of Freedom and the National Medal of Science. In addition to The Double Helix, his books include Molecular Biology of the Gene, Molecular Biology of the Cell, Recombinant DNA, and A Passion for DNA.
The structure of DNA proposed by James Watson and Francis Crick in 1953 was one of the most important scientific discoveries of the twentieth century, transforming biology, giving medicine new impetus, and providing a foundation for the new industry of biotechnology. Jim Watson’s best-selling memoir of the events leading to this discovery, The Double Helix, has enthralled millions of readers since its publication in 1968. In this videotaped lecture, recorded live at Cold Spring Harbor Laboratory in November 1999, Dr. Watson retraces those events in a very personal reminiscence of the period and the people involved. This is an account of one of the great dramas of science, told from the intimate perspective of a participant who, aged 25 at the time of the discovery, has become one of the intellectual leaders of our time.
James D. Watson was Director of Cold Spring Harbor Laboratory, New York, from 1968 to 1993 and is now its President. He was the first Director of the National Center for Human Genome Research of the National Institutes of Health from 1989 to 1992.
Dr. Watson is a member of the National Academy of Sciences and Royal Society and has received many awards and honors, including the Presidential Medal of Freedom and the National Medal of Science. In addition to The Double Helix, his books include Molecular Biology of the Gene, Molecular Biology of the Cell, Recombinant DNA, and A Passion for DNA.
In this volume of the most prestigious series in biology, published to mark the 40th anniversary of the Watson-Crick structure, 87 of the world's leading investigators discuss the most pressing questions in molecular genetics and cell biology. These include the expression of genes, DNA transcription, the structure and replication of genomes, the structure of chromosomes and the nucleus. A unique compilation of insights with lasting value.
Publication Date
June 1994/1993
Bibliographic Information 861 pp., illus., color plates, indexes
In this volume of the most prestigious series in biology, published to mark the 40th anniversary of the Watson-Crick structure, 87 of the world's leading investigators discuss the most pressing questions in molecular genetics and cell biology. These include the expression of genes, DNA transcription, the structure and replication of genomes, the structure of chromosomes and the nucleus. A unique compilation of insights with lasting value.
Publication Date
June 1994/1993
Bibliographic Information 861 pp., illus., color plates, indexes
DNA from the Beginning (a 3–CD set) is a multimedia primer on the basics of DNA, genes, and heredity. It is an ideal teaching tool for high school students and will also interest adults with a non-technical background who wish to learn more about the impact that DNA science is having on our lives.
The content in DNA from the Beginning is built on a progressive series of important but simply stated concepts such as Children resemble their parents. An understanding of the concept is then conveyed in clear,understandable terms with the aid of links to animations, photographs, and video clips, and biographies or interviews featuring key scientists. A wealth of other information and links to web resources are included. Problems and their solutions are included to allow teaching through self-examination.
Based on the award-winning web site, the DNA from the BeginningCD–ROM set provides over 72 hours of study time. It runs on Macintosh computers and PCs, and gives immediate access to all the online content, in attractive, uncluttered screens, without the need for network connections.
A must for high schools and junior colleges in conjunction with biology classes.
“DNA has come a long way.” James D. Watson, Nobel Laureate
DNA Interactive (DNAi) is a multimedia resource for high school and introductory college biology that shows how we came to understand the language of DNA, how we bend it to our own service, and what it can tell us about who we are. The DNAi products—an award winning website (http://www.dnai.org) and the DVD—are the result of a two–year collaboration between scientists, educators, and film–makers on three continents. It includes more than 200 video clips and animations; video interviews with 11 Nobel Laureates and more than 50 other scientists, clinicians, and patients; and spectacular 3–D animations that illustrate intracellular processes and explore aspects of DNA science.
Producer
Dolan DNA Learning Center, CSHL in collaboration with Red Green & Blue Company Ltd. Film footage by Windfall Films Ltd.
Publication Date
/2003
Bibliographic Information DVD disc (NTSC version—unencoded), 4 hours (approx.) of viewing
This is the third book in the colorful, award-winning series for children, which began with Cells Are Us and Cell Wars. The authors have a unique way of combining lively graphics, based on real scientific observations, with a clear, simple text. DNA Is Here To Stay explores the secret of life itself—the secret of DNA. The book is designed to teach young readers about the master plan that directs the growth of a single cell into a complete human being. (Ages 9-15)
Author
Fran Balkwill
Contributor
Mic Rolph
Publication Date
JANUARY 1991/1992
Bibliographic Information 32 fully illustrated 4-color pages
DNA microarray technology is a new and powerful means to analyze genomes and characterize patterns of gene expression. Its applications are widespread across the many fields of plant and animal biological and biomedical research. This manual, designed to extend and to complement the information in the best–selling Molecular Cloning, is a synthesis of the expertise and experience of more than 30 contributors—all innovators in a fast–moving field. DNA Microarrays provides authoritative, detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for analysis of images and data.
Editor
David Bowtell
Peter MacCallum Cancer Institute
Publication Date
2003/2003
Bibliographic Information 712 pp., illus., appendices, index
DNA microarray technology is a new and powerful means to analyze genomes and characterize patterns of gene expression. Its applications are widespread across the many fields of plant and animal biological and biomedical research. This manual, designed to extend and to complement the information in the best–selling Molecular Cloning, is a synthesis of the expertise and experience of more than 30 contributors—all innovators in a fast–moving field. DNA Microarrays provides authoritative, detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for analysis of images and data.
Editor
David Bowtell
Peter MacCallum Cancer Institute
Publication Date
2003/2003
Bibliographic Information 712 pp., illus., appendices, index
At least 5 trillion cell divisions are required for a fertilized egg to develop into an adult human, resulting in the production of more than 20 trillion meters of DNA! And yet, with only two exceptions, the genome is replicated once and only once each time a cell divides. How is this feat accomplished? What happens when errors occur? This book addresses these questions by presenting a thorough analysis of the molecular events that govern DNA replication in eukaryotic cells. The association between genome replication and cell proliferation, disease pathogenesis, and the development of targeted therapeutics is also addressed. At least 160 proteins are involved in replicating the human genome, and at least 40 diseases are caused by aberrant DNA replication, 35 by mutations in genes required for DNA replication or repair, 7 by mutations generated during mitochondrial DNA replication, and more than 40 by DNA viruses. Consequently, a growing number of therapeutic drugs are targeted to DNA replication proteins. This authoritative volume provides a rich source of information for researchers, physicians, and teachers, and will stimulate thinking about the relevance of DNA replication to human disease.
Editor
Melvin L. DePamphilis
National Institutes of Health
Publication Date
2006/2006
Bibliographic Information 814 pp., illus., appendices, index
DNA replication is a central cog in the machinery of cell and viral proliferation. After significant advances in the past few years, its regulation is now understood in unprecedented depth.
This is the first book to provide a detailed and thoroughly up-to-date review of the complexity of DNA replication in eukaryotic cells. It is organized into three parts: Concepts, a distillation of underlying principles; Enzymes, a description of each protein class involved; and Systems, a review of events over a wide range of organisms. The book is therefore invaluable for teachers who want a current survey of a topic central to the biology syllabus; investigators of replication who will appreciate a remarkably concise, central source of knowledge in their specialty; and scientists studying other biological functions on which DNA replication has an impact.
Editor
Melvin L. DePamphilis
National Institute of Child Health and Human Development, National Institutes of Health
Publication Date
July 1996/1996
Bibliographic Information 1058 pp., illus., color plates, index
This new laboratory text combines the theory, practice, and applications of recombinant DNA technology into one articulated package. Unlike super texts that can only be sampled by even the most ambitious instructor or student, DNA Science is designed to be read from cover to cover. The eight text chapters are written in a semi-journalistic style and adopt a historical perspective to explain where DNA science has come from and where it is going. Combining the unique perspectives of both a research biologist and a science writer, the topical treatment integrates up-to-the-minute examples drawn directly from the research literature.
Extensively tested by thousands of high school and college teachers and students in 25 states and Canada, the ten laboratory experiments cover the basic techniques of gene isolation and analysis. The experiments engender systematic repetition to build student confidence and mastery of techniques. Extensive prelab notes at the beginning of each experiment explain how to schedule and prepare, and flowcharts and icons make the protocols easy to follow. The laboratory course is completely supported by quality-assured Carolina Biological Supply Company products-from bulk reagents, to reusable reagent systems, to single-use kits-satisfying a range of teaching applications.
Truly a first course in recombinant DNA technology, the laboratory sequence presupposes no prior experience on the part of the instructor or student. Structured to follow directly from an introduction to principles of biology, the experiments are equally appropriate for the advanced high school student and the beginning college student. The book can be used as the first course in a molecular biology sequence, be integrated as a genetics/DNA structure component of a general biology course, or be used as a unit within a microbiology or genetics course. The text is suitable for introducing recombinant DNA in science and society courses.
Author
David A. Micklos
DNA Learning Center, Cold Spring HarborLaboratory
Publication Date
JANUARY 1990/1990
Bibliographic Information 477 pp., illus., bibliography, name index, glossary/index
This is the second edition of a highly successful textbook (over 50,000 copies sold) in which a highly illustrated, narrative text is combined with easy–to–use thoroughly reliable laboratory protocols. It contains a fully up–to–date collection of 12 rigorously tested and reliable lab experiments in molecular biology, developed at the internationally renowned Dolan DNA Learning Center of Cold Spring Harbor Laboratory, which culminate in the construction and cloning of a recombinant DNA molecule.
Proven through more than 10 years of teaching at research and nonresearch colleges and universities, junior colleges, community colleges, and advanced biology programs in high school, this book has been successfully integrated into introductory biology, general biology, genetics, microbiology, cell biology, molecular genetics, and molecular biology courses.
The first eight chapters have been completely revised, extensively rewritten, and updated. The new coverage extends to the completion of the draft sequence of the human genome and the enormous impact these and other sequence data are having on medicine, research, and our view of human evolution. All sections on the concepts and techniques of molecular biology have been updated to reflect the current state of laboratory research.
The laboratory experiments cover basic techniques of gene isolation and analysis, honed by over 10 years of classroom use to be thoroughly reliable, even in the hands of teachers and students with no prior experience. Extensive prelab notes at the beginning of each experiment explain how to schedule and prepare, while flow charts and icons make the protocols easy to follow.
As in the first edition of this book, the laboratory course is completely supported by quality–assured products from the Carolina Biological Supply Company, from bulk reagents, to useable reagent systems, to single–use kits, thus satisfying a broad range of teaching applications.
Author
Greg A. Freyer
Columbia University, New York
Publication Date
2003/2003
Bibliographic Information 575 pp., illus., appendices, index
The application of DNA technology in forensic science has generated considerable excitement, both in the criminal justice community and in the popular media. The first flush of excitement is now giving way to the realities of trying to implement these techniques, including the problems of transforming a research technique into a routine procedure and a concern for the legal and ethical issues involved. The objective of this meeting was to examine some of the key questions of policy relating to DNA technology and forensic science. For example: Should DNA taken for identification be used to determine other genetic characteristics? Should there be different standards of admissibility for DNA evidence compared with other types of evidence? Should there be regulation or accreditation of laboratories that provide DNA services and should it be on a state or national level? Data banks will be established for DNA information—how will these be controlled? This book marks the coming together of molecular biologists, legal authorities, forensic scientists, and policy analysts in a workshop setting. The proceedings of the meeting provide useful and unusual perspectives on this exciting area, and serve as the starting point for further developments.
Editor
John Ballantyne
Office of Medical Examiner, County ofSuffolk
Publication Date
JANUARY 1989/1989
Bibliographic Information 368 pp., illus., indexes
Continuous changes occur in the coiling, supercoiling, and catenation of DNA molecules during gene transcription and cell division. The activity of DNA is thus heavily influenced by its topology, yet this influence is not widely appreciated or understood. This book explains the biological implications of DNA superstructure for readers who are presumed not to know higher mathematics. It is an introductory text, but not an elementary one, and will be valuable for molecular biologists at graduate student level and beyond.
Editor
Nicholas R. Cozzarelli
University of California, Berkeley
Publication Date
JANUARY 1990/1990
Bibliographic Information 480 pp., illus., appendix, index
The DNA tumor viruses have long served as model systems for the study of eukaryotic gene expression, DNA replication, and transformation. DNA Tumor Viruses: Control of Gene Expression and Replication presents findings in the molecular biology and biochemistry of simian virus 40, polyomavirus, adenoviruses, papillomaviruses, herpes simplex virus, and Epstein-Barr virus.
Enhancing this collection of research reports are extensive introductions written by leading investigators, who review past progress in the study of the DNA tumor viruses, starting where the now-classic DNA Tumor Viruses, edited by John Tooze, leaves off.
DNA Tumor Viruses will be of vital interest to researchers active in the study of the expression and regulation of eukaryotic genes as well as to students seeking an introduction to the field.
Editor
Michael Botchan
University of California, Berkeley
Publication Date
JANUARY 1986/1986
Bibliographic Information 620 pp., illus., indexes
The first biography of the Nobel-prize winning chemist and peace activist, this book is a winning portrait of an accomplished woman who combined an ambitious career with family responsibilities, often at great cost.
Author
Georgina Ferry
Publication Date
December 2000/2000
Bibliographic Information 423 pp., illus., bibliography, index
The first biography of the Nobel-prize winning chemist and peace activist, this book is a winning portrait of an accomplished woman who combined an ambitious career with family responsibilities, often at great cost.
Author
Georgina Ferry
Publication Date
September 2000/2000
Bibliographic Information 423 pp., illus., bibliography, index
"...the point of this story, I'll tell you right now.
Did you ever sit down and think about how
It is that each time a baby's born
It's a baby -- not a rabbit or an ear of corn?"
The answer is in DNA -- the stuff that genes are made of. Twin brothers Joel and Ira Herskowitz, physician and scientist, respectively, have come up with a new way to explain to children (ages 8+) how it is that when a person's genetic message is "copied," the result is another human being -- not a rabbit or an ear of corn.
Based on a song written by Joel Herskowitz while he was in medical school, the "Double Talking Helix Blues" is a book and audio tape presentation on the structure and function of DNA. The book includes the text for Joel's song, which is illustrated with spectacular paintings by Judy Cuddihy to provide an important visual component to the overall "lesson" on DNA.
This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.
The outstanding features of this protocol collection are:
Scope: The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development.
Depth: Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators.
Format: Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable.
Added value: The book’s appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.
An evolution of Michael Ashburner’s 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.
Author
William Sullivan
University of California, Santa Cruz
Publication Date
March 2000/2000
Bibliographic Information 728 pp., illus., appendices, index
This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.
The outstanding features of this protocol collection are:
Scope: The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development.
Depth: Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators.
Format: Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable.
Added value: The book’s appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.
An evolution of Michael Ashburner’s 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.
Author
William Sullivan
University of California, Santa Cruz
Publication Date
March 2000/2000
Bibliographic Information 728 pp., illus., appendices, index
The second edition of the highly successful “grey book” Drosophila: A Laboratory Handbook, first published in 1989, has been completely updated to reflect advances in techniques and knowledge. The original format is maintained, providing a handy reference guide to most aspects of the biology of Drosophila. Each of the thirty–six chapters summarizes the present state of the subject, written with a historical perspective, but with an emphasis on the practical use of genetic and other methods. Extensively referenced.
Author
Michael Ashburner
University of Cambridge
Publication Date
2005/2005
Bibliographic Information 1409 pp., illus., appendices, index
Barbara McClintock was born in 1902, within a few years of the rediscovery of Mendel's laws. Her life, discoveries, and insights span the history of genetics in this century.
In the 1920s, she became a dominant figure in the group that flourished at Cornell University under R.A. Emerson and made remarkable technical and conceptual advances in maize cytogenetics. These studies continued at the California Institute of Technology, in Freiburg, Germany, and at the University of Missouri. In 1942, she joined the staff of the Carnegie Institution of Washington at Cold Spring Harbor, New York, where she became a Distinguished Service Member.
McClintock's unique ability to discern relationships between the behavior of chromosomes and the properties of the whole organism earned her early recognition. She was elected to the National Academy of Sciences in 1944 and to the presidency of the Genetics Society of America in 1945. Had she done no more, McClintock would have become a major figure in the history of genetics.
But at Cold Spring Harbor, she began the studies of the consequence of dicentric chromosome formation and breakage that led her to the discovery of genetic elements capable of moving within the genome and controlling expression of other genes. Although McClintock was universally respected and admired, the first reaction to these findings was often uncomprehending or indifferent, even dismissive. In due course, however, the generality of mobile genetic elements and the concept of a dynamic genome were understood and widely accepted, culminating in the award to McClintock of an unshared Nobel Prize in 1983.
As Barbara's 90th birthday approached, some of her many friends and colleagues were invited to write essays for the occasion. This book contains a kaleidoscope of contributions, many by those who discovered transposition in other organisms. Their essays give a remarkable account of the scientific legacy of one of the century's greatest geneticists.
Editor
Nina Fedoroff
Carnegie Institution of Washington
Publication Date
JANUARY 1992/1992
Bibliographic Information 422 pp., illus, indexes
This book has been produced using print on demand technology.
Yeast genetics began with Winge's 1935 studies of S. cerevisiae in Copenhagen, and afterwards was pursued by Lindegren in the U.S. and Ephrussi in France. Genetic studies in S. pombe were pioneered by Leupold in the 1940s in Switzerland. Within four decades, not without controversies, both yeast species were recognized as essential models in eukaryotic molecular cell biology. In this remarkable volume, Hall and Linder have assembled the reminiscences of many early investigators whose pioneering studies in the years before 1975 brought yeast biology to its current maturity. These illustrated essays about the science, the events and the personalities involved capture a fascinating era, in the informal style made famous by Phage and the Origins of Molecular Biology. This is a book that all scientists interested in the development of modern genetics and molecular biology should have on their shelves.
Editor
Michael N. Hall
Biozentrum der Universität Basel
Publication Date
JANUARY 1993/1993
Bibliographic Information 477 pp., illus., color plate, indexes
Yeast genetics began with Winge's 1935 studies of S. cerevisiae in Copenhagen, and afterwards was pursued by Lindegren in the U.S. and Ephrussi in France. Genetic studies in S. pombe were pioneered by Leupold in the 1940s in Switzerland. Within four decades, not without controversies, both yeast species were recognized as essential models in eukaryotic molecular cell biology. In this remarkable volume, Hall and Linder have assembled the reminiscences of many early investigators whose pioneering studies in the years before 1975 brought yeast biology to its current maturity. These illustrated essays about the science, the events and the personalities involved capture a fascinating era, in the informal style made famous by Phage and the Origins of Molecular Biology. This is a book that all scientists interested in the development of modern genetics and molecular biology should have on their shelves.
Editor
Michael N. Hall
Biozentrum der Universität Basel
Select
Patrick Linder
Biozentrum der Universität Basel
Publication Date
JANUARY 1993/1993
Bibliographic Information 477 pp., illus., color plate, indexes
Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis, which is easy to keep and ovulates at any time of year in response to simple hormone injections.
Xenopus embryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopus development has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation.
This manual contains a comprehensive collection of protocols for the study of early development in Xenopus embryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere.
This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopus and vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapes which are cross-referenced to the appropriate section of the manual.
Author
Hazel L. Sive
Whitehead Institute for Biomedical Research
Publication Date
November 1999/2000
Bibliographic Information 338 pp., illus., color plate, appendices, index
Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis, which is easy to keep and ovulates at any time of year in response to simple hormone injections.
Xenopus embryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopus development has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation.
This manual contains a comprehensive collection of protocols for the study of early development in Xenopus embryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere.
This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopus and vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapes which are cross-referenced to the appropriate section of the manual.
Author
Hazel L. Sive
Whitehead Institute for Biomedical Research
Publication Date
November 1999/2000
Bibliographic Information 338 pp., illus., color plate, appendices, index
In this classic, originally published 25 years ago and now reprinted with a new Afterword by the author on how he came to write the book, Judson tells the story of the birth and early development of molecular biology, in the US, the UK and France. In particular, the fascinating account of the remarkable golden period from the revelation of the double helix structure of DNA through to cracking the genetic code and solving the basic problems of how genes are regulated, is told largely in the words of the main players in the unfolding drama, all of whom were interviewed extensively by Judson in preparing this acclaimed volume.
As well as the new Afterword that appears here for the first time, the current edition contains the material added by the author to the earlier "Expanded Edition" (CSHL Press 1996) on some of the principal figures involved, particularly Rosalind Franklin, together with the Afterword added at that time which sketches the further development of molecular biology into the era of recombinant DNA.
Author
Horace Freeland Judson
Publication Date
November 1996/1996
Bibliographic Information 714 pp., illus., index, appendix, notes
The use of pulsed-field gel electrophoresis to manipulate large DNA molecules represents one of the most important recent technical breakthroughs in molecular biology. It has extended the resolving power of gel electrophoresis and bridged the gap between cytogenetic techniques and methods for detailed physical mapping. In the past five years, the techniques used have improved and a great deal has been learned about the principles underlying the movement of large DNA molecules through a gel. Until recently, much of the research has focussed on instrument improvement and the practical applications of the techniques. This volume is the first to combine what has been learned from years of running pulsed-field gels with new direct observations of DNA molecules in a gel, the latest theoretical studies, and computer modeling, giving the clearest view yet offered of the process concerned. It also contains the first attempt to define formulae describing the mobility and resolution of DNA in pulsed-field gels, the most recent pulsed regimens, and discussion on the application of the technique to biological systems.
“…it is interesting to know how phenomena were discovered, how the problems were born, attacked and solved, and how and why our ideas have evolved. The danger of parachuting young enthusiastic scientists into a flower bed of selected data and fully bloomed conceptions should not be underestimated.”
Bacteriology and genetics began as independent fields of study but converged in the 1930s in a series of stunning research achievements. Within 20 years, bacterial genetics would provide the tools and concepts on which molecular biology was founded.
In this book, Thomas Brock, author of a widely praised biography of Robert Koch, highlights and analyzes the experimental work that drove bacterial genetics forward.
Concentrating on the science rather than the personalities involved, Brock discusses key data from original sources, illuminating his analysis with unpublished material and insights from conversations with surviving investigators.
He has written a readable account of an important aspect of biology not covered in other books. It will appeal to everyone interested in the development of today's molecular biology.
Author
Thomas D. Brock
University of Wisconsin, Madison
Publication Date
JANUARY 1990/1990
Bibliographic Information 346 pp., illus., indexes
Until recently, a small number of model organisms has been the focus of most research in molecular, cellular, and developmental biology. But in the last few years, due in part to increased interest in questions of evolution, technical advances in selectively altering gene expression patterns, and the reduced costs of genome sequencing, the range of organisms used for research is greatly expanding. Emerging Model Organisms, Volume 1, introduces the reader to this new generation of model organisms, providing a diverse catalog of potential species useful for extending research in new directions. In this volume leading experts provide chapters on 23 emerging model systems, ranging from bat and butterfly to cave fish and choanoflagellates; cricket and finch to quail, snail, and tomato. Subsequent releases of the Emerging Model Organisms series, already in preparation, will focus on additional species. Material is also available in CSH Protocols: www.cshprotocols.org/emo
Publication Date
2009/2009
Bibliographic Information 592 pp., illus., appendix, index
Until recently, a small number of model organisms has been the focus of most research in molecular, cellular, and developmental biology. But in the last few years, due in part to increased interest in questions of evolution, technical advances in selectively altering gene expression patterns, and the reduced costs of genome sequencing, the range of organisms used for research is greatly expanding. Emerging Model Organisms, Volume 1, introduces the reader to this new generation of model organisms, providing a diverse catalog of potential species useful for extending research in new directions. In this volume leading experts provide chapters on 23 emerging model systems, ranging from bat and butterfly to cave fish and choanoflagellates; cricket and finch to quail, snail, and tomato. Subsequent releases of the Emerging Model Organisms series, already in preparation, will focus on additional species. Material is also available in CSH Protocols: www.cshprotocols.org/emo
Publication Date
2009/2009
Bibliographic Information 592 pp., illus., appendix, index
Beneath your skin there is an amazing hidden world of living cells. Millions and millions of cells work together, to make everything that is you. But did you know that you started life as just one tiny cell? And did you know that everything that lives on Planet Earth is also made of these amazing cells?
Author
Fran Balkwill
Publication Date
November 2001/2002
Bibliographic Information 32 fully illustrated 4-color pages
Beneath your skin there is an amazing hidden world of living cells. Millions and millions of cells work together, to make everything that is you. But did you know that you started life as just one tiny cell? And did you know that everything that lives on Planet Earth is also made of these amazing cells?
Author
Fran Balkwill
Publication Date
November 2001/2002
Bibliographic Information 32 fully illustrated 4-color pages
Many inheritable changes in gene function are not explained by changes in the DNA sequence. Such epigenetic mechanisms are known to influence gene function in most complex organisms and include effects such as transposon function, chromosome imprinting, yeast mating type switching and telomeric silencing. In recent years, epigenetic effects have become a major focus of research activity. This monograph, edited by three well-known biologists from different specialties, is the first to review and synthesise what is known about these effects across all species, particularly from a molecular perspective, and will be of interest to everyone in the fields of molecular biology and genetics.
Editor
Vincenzo E.A. Russo
Max-Planck-Institut für MolekulareGenetik
Publication Date
December 1996/1996
Bibliographic Information 692 pp., illus., color plates, glossary, index
The regulation of gene expression in many biological processes involves epigenetic mechanisms. In this new volume, 24 chapters written by experts in the field discuss epigenetic effects from many perspectives. There are chapters on the basic molecular mechanisms underpinning epigenetic regulation, discussion of cellular processes that rely on this kind of regulation, and surveys of organisms in which it has been most studied. Thus, there are chapters on histone and DNA methylation, siRNAs and gene silencing; X-chromosome inactivation, dosage compensation and imprinting; and discussion of epigenetics in microbes, plants, insects, and mammals. The last part of the book looks at how epigenetic mechanisms act in cell division and differentiation, and how errors in these pathways contribute to cancer and other human diseases. Also discussed are consequences of epigenetics in attempts to clone animals. This book is a major resource for those working in the field, as well as being a suitable text for advanced undergraduate and graduate courses on gene regulation.
Editor
C. David Allis
The Rockefeller University, New York
Publication Date
2007/2007
Bibliographic Information 502 pp., illus., appendices, index
The importance of epigenetic regulation of gene expression, particularly in higher organisms, is now clear and the 2004 Cold Spring Harbor Annual Symposium provided many new examples as well as insights into the underlying mechanisms. The resulting volume—with over 60 papers from experts across the field—covers various aspects of nuclear organization and dynamics; genomic imprinting, chromosomal inactivation, and other examples of gene silencing; the histone and DNA modifications associated with these conditions; and the roles of RNA and protein regulators in establishing and maintaining these states.
Editor
Bruce Stillman
Cold Spring Harbor Laboratory
Publication Date
2004/2004
Bibliographic Information 532 pp., illus., index (Paperback edition does not include online access)
The importance of epigenetic regulation of gene expression, particularly in higher organisms, is now clear and the 2004 Cold Spring Harbor Annual Symposium provided many new examples as well as insights into the underlying mechanisms. The resulting volume—with over 60 papers from experts across the field—covers various aspects of nuclear organization and dynamics; genomic imprinting, chromosomal inactivation, and other examples of gene silencing; the histone and DNA modifications associated with these conditions; and the roles of RNA and protein regulators in establishing and maintaining these states.
Editor
Bruce Stillman
Cold Spring Harbor Laboratory
Publication Date
2004/2004
Bibliographic Information 532 pp., illus., index (Hardcover edition includes online access; call for information and registration)
The regulation of gene expression in many biological processes involves epigenetic mechanisms. In this new volume, 24 chapters written by experts in the field discuss epigenetic effects from many perspectives. There are chapters on the basic molecular mechanisms underpinning epigenetic regulation, discussion of cellular processes that rely on this kind of regulation, and surveys of organisms in which it has been most studied. Thus, there are chapters on histone and DNA methylation, siRNAs and gene silencing; X-chromosome inactivation, dosage compensation and imprinting; and discussion of epigenetics in microbes, plants, insects, and mammals. The last part of the book looks at how epigenetic mechanisms act in cell division and differentiation, and how errors in these pathways contribute to cancer and other human diseases. Also discussed are consequences of epigenetics in attempts to clone animals. This book is a major resource for those working in the field, as well as being a suitable text for advanced undergraduate and graduate courses on gene regulation.
Editor
C. David Allis
The Rockefeller University, New York
Publication Date
2007/2007
Bibliographic Information 502 pp., illus., appendices, index
Published in 1998, the three-volume lab manual Cells contained a comprehensive collection of techniques for studying cellular structure and function. This new CD-ROM,Essentials from Cells: A Laboratory Manual, presents a selection from the book of methods with proven reliability that every investigator working with cellular systems should have available. Essentials from Cells: A Laboratory Manual provides precisely crafted, step–by–step protocols and essential background details in a readily accessible, searchable electronic format.
Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. They participate in activities as diverse as protein folding, leukocyte physiology, and microbial pathogenesis. Genetic defects in their assembly are at the root of a growing list of human diseases. Essentials of Glycobiology is an ideal gateway to the understanding of glycans, and is the first textbook approach to describing their biogenesis and function. Written and edited by glycobiologists with experience in both teaching and research, this volume will be invaluable for students and established investigators in developmental biology, cell biology, neuroscience, immunology, and biochemistry, who need a complete yet concise introduction to this burgeoning field.
Contributor
Maarten Chrispeels
University of California, San Diego
Editor
Ajit Varki
University of California, San Diego
Publication Date
August 1999/1999
Bibliographic Information 653 pp., illus., glossary, index
Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. They participate in activities as diverse as protein folding, leukocyte physiology, and microbial pathogenesis. Genetic defects in their assembly are at the root of a growing list of human diseases. Essentials of Glycobiology is an ideal gateway to the understanding of glycans, and is the first textbook approach to describing their biogenesis and function. Written and edited by glycobiologists with experience in both teaching and research, this volume will be invaluable for students and established investigators in developmental biology, cell biology, neuroscience, immunology, and biochemistry, who need a complete yet concise introduction to this burgeoning field.
Contributor
Maarten Chrispeels
University of California, San Diego
Editor
Ajit Varki
University of California, San Diego
Publication Date
1999/1999
Bibliographic Information 653 pp., illus., glossary, index
Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. They participate in activities as diverse as protein folding, leukocyte physiology, and microbial pathogenesis. Genetic defects in their assembly are at the root of a growing list of human diseases. Essentials of Glycobiology is an ideal gateway to the understanding of glycans, and is the first textbook approach to describing their biogenesis and function. Written and edited by glycobiologists with experience in both teaching and research, this volume will be invaluable for students and established investigators in developmental biology, cell biology, neuroscience, immunology, and biochemistry, who need a complete yet concise introduction to this burgeoning field.
Contributor
Maarten Chrispeels
University of California, San Diego
Editor
Ajit Varki
University of California, San Diego
Publication Date
August 1999/1999
Bibliographic Information 653 pp., illus., glossary, index
The sugar chains of cells—known collectively as glycans—play a variety of impressive, critical, and often surprising roles in biological systems. Glycobiology is the study of the roles of glycans in the growth and development, function, and survival of an organism. Glyco-related processes, described in vivid detail in the text, have become increasingly significant in many areas of basic research as well as biomedicine and biotechnology.
This new edition of Essentials of Glycobiology covers the general principles and describes the structure and biosynthesis, diversity, and function of glycans and their relevance to both normal physiologic processes and human disease. Several new chapters present significant advances that have occurred since the publication of the first edition. Three sections of note describe organismal diversity, advances in our understanding of disease states and related therapeutic applications, and the genomic view of glycobiology. “Glycomics,” analogous to genomics and proteomics, is the systematic study of all glycan structures of a given cell type or organism and paves the way for a more thorough understanding of the functions of these ubiquitous molecules.
The first edition of Essentials of Glycobiology represented also a notable experiment in publishing, as it became one of the first electronic textbooks. And, now, in recognition of its wide audience and the changing ways in which researchers and students learn and access information, the new edition of Essentials will be made available online simultaneously with the print edition. This novel experiment is the result of the collaborative efforts of the Cold Spring Harbor Laboratory Press, the National Center for Biotechnology Information, and the editors of the book. Written and edited by glycobiologists with experience in teaching and in research, this volume will be an invaluable resource, both for students and for established investigators in fields such as developmental biology, cell biology, neuroscience, immunology, and biochemistry who require a complete yet concise introduction to this burgeoning field.
Advance praise for the Second Edition:
“The basic principles of glycobiology are clearly articulated in this volume, and the roles of complex carbohydrates in disease are an important read for all biomedical scientists.”
—Peter Agre, M.D., Nobel Laureate in Chemistry, 2003
“Essentials of Glycobiology is a major resource for understanding these post-translational biochemical reactions that affect the function and fate of proteins produced by the genes that are profoundly changed by their added sugars.”
—Baruch S. Blumberg, Nobel Laureate in Medicine, 1976
“The second edition of Essentials of Glycobiology, superbly printed and illustrated, develops in simple and absolutely precise terms the complicated intricacies of glycobiology. I would have killed to get this encyclopedic treatise 40 years ago when I was working my way through this field.”
—Edmond H. Fischer, Nobel Laureate in Medicine, 1992
Editor
Ajit Varki
University of California, San Diego
Publication Date
2009/2009
Bibliographic Information 784 pp., illus. glossary, study guide, index
Understanding the mechanism and regulation of eukaryotic DNA replication remains a fundamental area of modern biological research. Replication of the cell's genome is one of the critical, regulated events that occur during cell proliferation and cell cycle progression. This volume presents important advances in this field that were discussed at the Cold Spring Harbor Cancer Cells meeting on Eukaryotic DNA Replication. The papers cover topics as diverse as viral DNA replication, genome amplification, cell cycle and developmental control of DNA replication, replication proteins, and the replication and function of centromeres and telomeres. This volume is a valuable resource for researchers and students.
Editor
Thomas Kelly
Johns Hopkins University School ofMedicine
Publication Date
JANUARY 1988/1988
Bibliographic Information 489 pp., illus., indexes
Evolution is a new book on evolutionary biology that integrates molecular biology, genomics, and human genetics with traditional studies of evolutionary processes.
Recommended as a primary textbook for undergraduate courses in evolution
Required reading for biologists seeking a clear, current, and comprehensive account of evolutionary theory and mechanisms
Written by experts in population genetics, bacterial genomics, paleontology, human genetics, and developmental biology
Integrates molecular and evolutionary biology in ways that reflect current directions in research
Contents and Coverage
This extensively illustrated, full-color book has four sections:
Introduction (Part I) gives an account of how the ideas underpinning evolutionary theory developed and a history of experiments and ideas in the development of molecular biology.
Origin and Diversification of Life (Part II) describes the history of life on earth from the origin of life to the evolution of humans, with emphasis on the major transitions in genetic organization and novel adaptations that have appeared. The diversity of life is emphasized. The chapters make extensive use of information from complete genome sequences and analysis of molecular mechanisms in development.
Evolutionary Processes (Part III) describes how the diversity of life is generated: How variation arises and how selection acts are considered in detail. Many examples used to illustrate these processes are drawn from molecular sources.
Human Evolution (Part IV) discusses human evolution and diversity. The benefits of molecular markers for our understanding of human evolution are highlighted and these findings integrated with paleontological evidence. Also discussed is the use of evolutionary methods to identify genetic differences that predispose people to specific diseases and affect their responses to treatment.
Online-only Chapters
Additional chapters, found on the Web only, deal with techniques and models used in studying evolutionary biology, emphasizing the contribution of molecular biology and genomics to phylogenetic reconstruction methods.
Resources for Instructors
The Evolution Web site (www.evolution-textbook.org) is an invaluable supplement to the textbook, a resource for teachers that will contain downloadable figures (for PowerPoint or overhead display) and chapter problems.
Request exam copies and other information
Visit the Evolution Web site now for more information about this new book. Request a detailed Table of Contents, Sample Chapters, Exam Copies, and Updates about Evolution.
Author
Nicholas H. Barton
University of Edinburgh
Publication Date
2007/2007
Bibliographic Information 833 pp., color illus., glossary, index Price subject to change
The effective design of scientific experiments is critical to success, yet graduate students receive very little formal training in how to do it. Based on a well–received course taught by the author, Experimental Design for Biologists fills this gap.
Experimental Design for Biologists explains how to establish the framework for an experimental project, how to set up a system, design experiments within that system, and how to determine and use the correct set of controls. Separate chapters are devoted to negative controls, positive controls, and other categories of controls that are perhaps less recognized, such as “assumption controls,” and “experimentalist controls.” Furthermore, there are sections on establishing the experimental system, which include performing critical “system controls.”
Should all experimental plans be hypothesis–driven? Is a question/answer approach more appropriate? What was the hypothesis behind the Human Genome Project? What color is the sky? How does one get to Carnegie Hall? The answers to these kinds of questions can be found in Experimental Design for Biologists. Written in an engaging manner, the book provides compelling lessons in framing an experimental question, establishing a validated system to answer the question, and deriving verifiable models from experimental data. Experimental Design for Biologists is an essential source of theory and practical guidance in designing a research plan.
Author
David J. Glass, M.D.
Novartis Institutes for Biomedical Research, Cambridge, Massachusetts
Andy O'Hara, a post-doctoral researcher in a London cancer research institute, agrees to help an attractive colleague, Gina, who is collaborating with a pharmaceutical company. She is working on a virus-based vaccine but has discovered the company is planning a clinical trial of the vaccine in Africa without preliminary animal tests. Andy gets a tip-off that Gina's corporate collaborator has a shady past, but also discovers a scientific reason why the vaccine may be doomed to failure. As this excerpt begins, Andy and his friend Christine have helped Gina set up an illicit mouse experiment in their own animal facility, a move that could end all their careers if discovered.
Sixty-two experiments are set forth in step-by-step detail, including lists of required materials and complete characterization of the 79 strains of E. coli used. For additional experiments, see the sequel to this volume, A Short Course in Bacterial Genetics
Author
J.H. Miller
Harvard Society of Fellows and University of Geneva
Publication Date
/1972
Bibliographic Information 468 pp., illus., color plates
Schizosaccharomyces pombe, the fission yeast, is an important tool for studying the genetic regulation of cell division and other aspects of cell and molecular biology. Based on a practical laboratory course on techniques using S. pombe, given for three years at Cold Spring Harbor Laboratory, this book is the only available techniques manual for fission yeast. It consists of tried and tested experiments devised by experts in the field.
In addition to clearly explained step-by-step protocols, the manual provides the reader with an overview of the cell biology, molecular biology, and genetics of S. pombe. The book enables scientists with little or no experience in working with fission yeast to carry out the techniques necessary to design research projects using the organism.
Author
Caroline Alfa
University College London
Publication Date
JANUARY 1993/1993
Bibliographic Information 188 pp., illus., color plates, appendices
Experiments with Drosophila give investigators the chance to combine molecular and genetic approaches in studies of gene expression, cell function, and development. Fly genetics has a large and complex folklore that, for the uninitiated, is quite daunting. In this eagerly awaited book, Ralph Greenspan provides a bridge to that folklore. Entertaining and lucid, he guides the reader carefully through the practicalities of making crosses, isolating variants, mapping genes, constructing specific genotypes, and analyzing mutations. No previous knowledge of fly genetics is assumed. The techniques used are illustrated, and practice problems and solutions are included to assist the reader. This is an affordable and uniquely valuable book, ideal for graduate students starting projects and established investigators considering new avenues. A must for both personal and laboratory libraries.
Author
Ralph J. Greenspan
New York University
Publication Date
January 1997/1997
Bibliographic Information 155 pp., illus., appendices, index
A new edition of the classic handbook that has become a standard in the Drosophila field. The book is expanded to include topics in which classical genetic strategies have been augmented with new molecular tools. Included are such new techniques as homologous recombination, RNAi, new mapping techniques, and new mosaic marking techniques.
Author
Ralph J. Greenspan
The Neurosciences Institute, San Diego
Publication Date
2004/2004
Bibliographic Information 191 pp., illus., appendices, index
This engrossing biography by one of molecular biology’s foremost scholars reveals the remarkable evolution of Francis Crick’s scientific career and the shaping of his personality. From unpromising beginnings, he became a vital contributor to a remarkably creative period in science. Olby chronicles Crick’s life from his early studies in biophysics, to the discovery of the structure of DNA, to his later work in neuroscience and the nature of consciousness. This account is woven together with insights into his personal life gained through access to Crick’s papers, family, and friends. Robert Olby’s book is a richly detailed portrait of one of the great scientists of our time.
Author
Robert Olby
Publication Date
August 2009/2008
Bibliographic Information 450 pp. (approx.), illus., indexes
From a to α is a short supplemental textbook that uses control of yeast mating type as a model for many aspects of cell determination in general. Topics covered include gene silencing; genetic recombination; differentiation; combinatorial gene regulation; mRNA transport to establish asymmetric cell division; signal transduction; evolution of genetic networks; and various aspects of cell biology, including action of cytoskeleton and bud site selection. The book includes a foreword by Mark Ptashne, author of A Genetic Switch.
From a to α is a short supplemental textbook that uses control of yeast mating type as a model for many aspects of cell determination in general. Topics covered include gene silencing; genetic recombination; differentiation; combinatorial gene regulation; mRNA transport to establish asymmetric cell division; signal transduction; evolution of genetic networks; and various aspects of cell biology, including action of cytoskeleton and bud site selection. The book includes a foreword by Mark Ptashne, author of A Genetic Switch.
Deciphering Nature’s Alphabet is a compilation of five 10–minute films, which celebrate major achievements in genetic science from the discovery of DNA, fifty years ago, to the mapping of the human genome today. Through intimate interviews with some of the key players in genetics, each film explores a distinct historical period, its scientific breakthroughs and how they set the stage for the next phase of discovery.
On the occasion of the 50th anniversary of the discovery of DNA, State of the Art, Inc. (SOTA) developed these five films in high definition television format for the NHGRI (National Human Genome Research Institute). The films were screened as interstitial material, bracketed by lectures from key scientists at the Scientific Symposium on April 15, 2003, and were called the “jewel in the crown” of the symposium. They are now available in a single DVD, with total viewing time of 60 minutes. This DVD can be viewed on a Windows or MAC computer with a DVD drive, or a DVD player.
Producer
Gerardine Wurzburg
President of SOTA (State of the Art, Inc.) Published by SOTA (State of the Art, Inc.) and the NHGRI (National Human Genome Research Institute)
Publication Date
2003/2003
Bibliographic Information DVD disc, NTSC version, region unencoded
Much is being learned about how the brain works by studies of diseases that affect it. This ambitious volume portrays some of the most exciting aspects of neuroscience today—language development, visual awareness, neuronal plasticity, sensory perception, memory formation—by presenting studies on their normal mechanisms alongside illuminating investigations of abnormalities caused by degenerative disease, addiction, developmental errors, and other maladies. In this sixty-first volume of the most prestigious book series in experimental biology, over eighty of the world's most distinguished investigators provide a perspective that resonates beyond the lab bench into the clinics of the not-so-distant future.
Publication Date
May 1997/1996
Bibliographic Information 875 pp., illus., color plates, appendices
Much is being learned about how the brain works by studies of diseases that affect it. This ambitious volume portrays some of the most exciting aspects of neuroscience today—language development, visual awareness, neuronal plasticity, sensory perception, memory formation—by presenting studies on their normal mechanisms alongside illuminating investigations of abnormalities caused by degenerative disease, addiction, developmental errors, and other maladies. In this sixty-first volume of the most prestigious book series in experimental biology, over eighty of the world's most distinguished investigators provide a perspective that resonates beyond the lab bench into the clinics of the not-so-distant future.
Publication Date
May 1997/1996
Bibliographic Information 875 pp., illus., color plates, appendices
This volume describes the advances that have been made in the study of gap junctions using the techniques of biochemistry, biophysics, and structural analysis. The control of gap junction formation, their role in intercellular communication and development, and synaptic interactions are examined in a wide range of cell types and organisms. This book will be a valuable resource for advanced undergraduate and graduate students and research scientists in neurobiology, physiology, anatomy, cell biology, and developmental biology.
Editor
Michael V.L. Bennett
Albert Einstein College of Medicine
Publication Date
JANUARY 1985/1985
Bibliographic Information 409 pp., illus., indexes
Set Info
Topics
Structure;Biochemistry;Biophysics;Control of Formation;Role of Intercellular Communication and Development;Electronic Synapses
During gastrulation, tissue layers are formed and the overall body plan is established. This book is the definitive guide to this vitally important period in embryonic development, providing authoritative and up to date information that includes the first comprehensive interspecies comparison, cell movements and patterning events, the roles of individual genes and gene families, and the evolution of gastrulation.
Editor
Claudio D. Stern
University College London
Publication Date
2004/2004
Bibliographic Information 731 pp., illus., appendices, index
In 1982, a meeting of unusual influence was held at the Banbury Conference Center of Cold Spring Harbor Laboratory. After an early attempt at treating clinical disease with transferred genes had ignited public attention and scientific controversy, a group of distinguished biologists and physicians came together to assess practical progress towards gene therapy and what its future might be. The geneticist Ted Friedmann wrote a narrative account of the participants' contributions to the meeting, ending with a personal discussion of ethical issues raised by genetic technologies.
His book, the first on gene therapy, was widely read but has long been unavailable. It has been reprinted with a new introduction by the author, in which he reviews the field's technical accomplishments and ethical dilemmas. Now that gene therapy has become part of the medical landscape, this volume is of interest as both a historical document and an assessment of the field's current challenges.
Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery.
Editor
Theodore Friedmann
University of California, San Diego
Publication Date
2007/2007
Bibliographic Information 793 pp., illus., appendix, index
Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery.
Editor
Theodore Friedmann
University of California, San Diego
Publication Date
2007/2007
Bibliographic Information 793 pp., illus., appendix, index
Genes & Signals analyzes gene regulation from a new perspective. The first chapter describes mechanisms found in bacteria, and two subsequent chapters discuss which of these is most highly exploited in higher organisms. A final chapter relates these molecular strategies to other enzymatic processes, including those involving kinases, RNA splicing enzymes, proteases, and others. A general theme emerges, one that proposes how a rather restricted set of signals and enzymatic functions has been used in evolution to generate complex life forms of different types.
Genes & Signals analyzes gene regulation from a new perspective. The first chapter describes mechanisms found in bacteria, and two subsequent chapters discuss which of these is most highly exploited in higher organisms. A final chapter relates these molecular strategies to other enzymatic processes, including those involving kinases, RNA splicing enzymes, proteases, and others. A general theme emerges, one that proposes how a rather restricted set of signals and enzymatic functions has been used in evolution to generate complex life forms of different types.
Cold Spring Harbor Laboratory Press has a limited supply of autographed copies of the hardcover edition of Genes, Girls, and Gamow, by James D. Watson, for distribution in the United States and Canada only. This represents a rare opportunity to own a book signed by one of this century’s most influential scientists.
FROM THE PUBLISHER (Alfred A. Knopf): Immediately following the revolutionary discovery of the structure of DNA by James D. Watson and Francis Crick in 1953, the world of molecular biology was caught up in a gold rush. The goal: to uncover the secrets of life that the newly elucidated molecule promised to reveal. Genes, Girls, and Gamow is James Watson’s report on the amazing aftermath of the DNA breakthrough, picking up where his now classic memoir, The Double Helix, leaves off.
Here are the collaborations and collisions of giants, not only Watson and Crick themselves, but also legions of others, including Linus Pauling (the greatest chemist of the day), Richard Feynman (the bongo–playing cynosure of Caltech), and especially George Gamow, the bearlike, whiskey–wielding Russian physicist, who had turned his formidable intellect to the field of genetics; with Gamow—an irrepressible prankster to boot—Watson would found the legendary RNA Tie Club.
But Watson—at twenty–five already the winner of genetic research’s greatest jackpot—is obsessed with another goal as well: to find love, and a wife equal to his unexpected fame. As he and an international cast of roguish young colleagues do important research they also compare notes and share complaints on the scarcity of eligible mates. And amid the feverish search for the role of the then still mysterious RNA molecule, Watson’s thoughts are seldom far from the supreme object of his desire, an enthralling Swarthmore coed who also happens to be the daughter of Harvard’s most eminent biologist.
Part scientific apprenticeship, part sentimental education, Genes, Girls, and Gamow is a penetrating revelation of how great science is accomplished. It is also a charmingly candid account of one young man’s full range of ambitions.
Author
James D. Watson
Cold Spring Harbor Laboratory
Publication Date
2001/2001
Bibliographic Information 336 pp., 43 illus., 22 letters NOTE: For distribution in the United States and Canada only.
The study of bacterial pathogens with genetic methods is a new and explosive field set to dominate microbiology in the next decade. Five years' progressive refinement in the celebrated Cold Spring Harbor course in Advanced Bacterial Genetics has produced a manual that teaches theoretical and practical molecular genetic approaches to bacterial pathogenicity. Chapters on concepts, technologies, and applications are followed by 15 multifaceted experiments with Salmonella and Vibrio, in which protocols and expected findings are fully demonstrated and strategies for similar approaches to other bacteria are discussed. This manual, the latest in a distinguished series from this long-established course, is the creation of three leading authorities on bacterial pathogens and is a conceptually unique book written for a broad audience of microbiologists in research, industrial, and public health labs.
Author
Stanley R. Maloy
University of Illinois
Publication Date
December 1995/1996
Bibliographic Information 603 pp., illus., appendices, index
The rapid development of molecular techniques for introducing single genes into plants has provided important prospects for genetically improving crop plants. Opportunities to make crops resistant to new herbicides that require low use rates and have low mammalian toxicity exist today. New resistance strategies for insects and viruses have been demonstrated in the field, and improved plant tolerance to fungal diseases is being developed. Improving the quality of plant proteins and oils are future prospects for the technology.
The commercial development of seed products incorporating these and other important new traits will require regulatory approval by USDA, EPA, or FDA. Those procedures have been developed for small-scale field tests of plants. The steps required for commercial development and sale of genetically engineered crop seeds or plants remain poorly defined.
This volume reports on a meeting held at Cold Spring Harbor's Banbury Center, which brought together molecular biologists, plant geneticists, economists, and government representatives from regulatory agencies and congress to discuss the prospects and issues important to the development of genetically improved seeds of agricultural crops.
Bibliographic Information 289 pp., illus., indexes
Set Info
Topics
Developmental Genetics;Endogenous Viruses and Viral Vectors;Introduction of Cloned Genes into the Mammalian Germline;Nonmammalian Systems;Concluding Remarks
Human genome research will be one of the dominant themes of science in the 1990s. To assist its progress, new technologies and concepts are expected to emerge from the analysis of other organisms' genes and chromosomes. Comparative data on the genetic organization of different species, therefore, have particular importance. Since 1980, Genetic Maps has been the only comprehensive source of such information. This new, sixth edition is published in two formats: as a series of six paperback volumes, each containing a variety of genetic maps from one group of organisms, and as a cloth-bound, reading-room edition containing the complete collection of maps from all 129 species listed.
Book 1 - Viruses
Book 2 - Bacteria, Algae, and Protozoa
Book 3 - Lower Eukaryotes
Book 4 - Nonhuman Vertebrates
Book 5 - The Human Maps
Book 6 - Plants
Editor
Stephen J. O'Brien
Laboratory of Viral Carcinogenesis,National Cancer Institute, Frederick,Maryland
Genetic Variation: A Laboratory Manual is the first compendium of protocols specifically geared towards genetic variation studies, and includes thorough discussions on their applications for human and model organism studies. Intended