News from CSHL Press

Multifunctional protein contributes to blood cell development
Salk researchers uncover mechanism for how blood cells mature and specialize—and why errors can sometimes lead to leukemia
Getting straight to the heart of the matter in stem cells
Salk scientists discover how to generate functioning cardiac cells from stem cells in single step
Clock gene may hold answers to human brain evolution
Scientists have long sought to unravel the molecular mysteries that make the human brain special: What processes drove its evolution through the millennia? Which genes are critical to cognitive development?
Women and Lung Cancer
Long-suspected connection between female sex hormones and development of cancer identified for the first time.
New book on Cell-Cell Junctions published by CSHLPress
Cold Spring Harbor Laboratory Press (CSHLP) announced the release of Cell-Cell Junctions, Second Edition, available on its website in hardcover and eBook formats.
Brain tumour’s 'addiction' to common amino acid could be its weakness
Starving a childhood brain tumour of the amino acid glutamine could improve the effect of chemotherapy, according to an early study led by Queen Mary University of London (QMUL) and funded by Children with Cancer UK and the Medical Research Council.
RNA stem-loop is crucial for the survival of male flies
In many species the sexes have different sets of chromosomes. In humans as well as in the fruit fly Drosophila females have two X chromosomes, while males have one X and one copy of the much smaller Y chromosome. The latter carries very few genes, while the X chromosome has thousands of genes. Male fruit flies compensate for this short-coming by doubling the activity of their single X chromosome in a process called “dosage compensation”. If male flies fail to compensate, they die!
Scientists Identify Key Regulator of Male Fertility
When it comes to male reproductive fertility, timing is everything. Now scientists are finding new details on how disruption of this timing may contribute to male infertility or congenital illness.
New book on Cancer Evolution from Cold Spring Harbor Laboratory Press
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of Cancer Evolution, available on its website in hardcover and ebook formats.
Breast cancer study reveals dynamics of estrogen receptor function
Targeting estrogen receptors with small-molecule drugs, known as antihormone therapy, is an effective way to treat ER-positive breast cancers that are dependent on the steroid hormone estrogen. However, treated tumors frequently become resistant to therapy, prompting the search for new ways to block receptor function.
Landmark Glycobiology textbook now available in print and online
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) today announced the publication of Essentials of Glycobiology, Third Edition, a comprehensive overview of glycobiology for the advanced undergraduate and beginning graduate-level student of molecular and cellular biology or biomedicine.
Targeting a Binding Protein in Mutated p53 Could Yield New Cancer Treatment Strategies
Research by Rutgers Cancer Institute of New Jersey investigators shows the targeting of a binding protein of mutant p53 known as Rac1 could lead to new therapeutic strategies for patients whose cancer carries mutations in the p53 gene.
APC Sets the Wnt Tone Necessary for Cerebral Cortical Progenitor Development
UNC School of Medicine researchers pinpoint signaling problems in the progenitor cells crucial for proper neuron generation and organization.
OMRF researchers identify gene mutation present in pair of diseases
Scientists at OMRF have discovered how mutations in one specific gene can cause two distinct and rare diseases.
Embryos tune into the right channel
Transcription is the first step of gene expression, the process by which the instructions in our DNA are converted into functional products, such as proteins. Transcription is a ‘noisy’ process, which means that it does not occur in all cells at the same time, even when cells receive the same signals. This is seemingly incompatible with the importance of gene expression. But zebrafish embryos don't seem to care much about noisy transcription; they just wait until the effects of noise fade, and live happily ever after.
New book on Prion Biology from Cold Spring Harbor Laboratory Press
Cold Spring Harbor, N.Y. -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of Prion Biology, available on its website in hardcover, paperback, and ebook formats.
New book on The Biology of the TGF-β Family from Cold Spring Harbor Laboratory Press
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of The Biology of the TGF-β Family, available on its website in hardcover and eBook formats.
Why we did not evolve to live forever: Unveiling the mystery of why we age
Scientists at IMB have made a breakthrough in the understanding of the origin of the ageing process. Their new paper recently published in Genes & Development identified that genes belonging to a process called autophagy – one of the cells most critical survival processes – promote health and fitness in young worms but drive the process of ageing later in life.
New book on Cell Polarity from Cold Spring Harbor Laboratory Press
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of Cell Polarity, available on its website in hardcover format.
Molecular Map Shows How to Disable Dangerous Bioweapon
American scientists studying F. tularensis recently mapped out the complex molecular circuitry that enables the bacterium to become virulent. The map reveals a unique characteristic of the bacteria that could become the target of future drug development.
New book on Tissue Engineering and Regenerative Medicine from CSHLPress
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of Tissue Engineering and Regenerative Medicine, available on its website in hardcover, paperback, and ebook formats.
New book on The Biology of Exercise from Cold Spring Harbor Laboratory Press
Cold Spring Harbor, NY - Cold Spring Harbor Laboratory Press (CSHLP) today announced the release of The Biology of Exercise, available on its website in hardcover, paperback, and ebook formats.
New book on Targeting Cancer from Cold Spring Harbor Laboratory Press
Cold Spring Harbor, NY - Cold Spring Harbor Laboratory Press (CSHLP) announced the release of Targeting Cancer, available on its website in hardcover, paperback, and eBook formats.
New book on Prion Diseases from Cold Spring Harbor Laboratory Press
Cold Spring Harbor, NY - Cold Spring Harbor Laboratory Press (CSHLP) today announced the release of Prion Diseases, available on its website in paperback and eBook formats.
Disrupted prenatal RNA processing and myogenesis in congenital myotonic dystrophy
For years, the underlying process that causes a debilitating muscle disorder in infants and young children has been largely unknown. Now, a group that includes University of Florida Health genetics researchers has identified the fundamental mechanism that causes congenital myotonic dystrophy.
Neuroblast differentiation during development and in neuroblastoma requires KIF1Bβ mediated transport of TRKA
A Ludwig Institute for Cancer Research Ltd. Karolinska Institutet news release on an article that was published in the May 15, 2017 issue of Genes & Development
New book on Synthetic Biology from Cold Spring Harbor Laboratory Press
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) today announced the release of Synthetic Biology: Tools for Engineering Biological Systems, available on its website in hardcover and eBook formats.
New book on Chromatin Deregulation in Cancer from CSHL Press
Written and edited by experts in the field, Chromatin Deregulation in Cancer from Cold Spring Harbor Perspectives in Medicine examines the chromatin components that are commonly mutated, what is understood about the mechanisms that lead to hematological malignancies and solid tumors, and prospects for the therapeutic modulation of chromatin.
Searching for a crystal-clear picture of a molecular complex linked to cancer, other diseases
A Van Andel Institute news release on an article that was published in the May 1, 2017 issue of Genes & Development
Hormone-dependent control of developmental timing through regulation of chromatin accessibility
In fruit fly and human genetics, timing is everything. UNC scientists show how DNA is accessed and used during the journey to maturation in fruit flies, and what this might mean to our understanding of how cancers arise.
New laboratory study of glioma links more aggressive immune response to poorer prognosis
A Fred Hutchinson Cancer Research Center news release on an article that was published in the April 15, 2017 issue of Genes & Development
Uncovering the Genetic Mechanisms Driving Embryonic Development
A Northwestern University Feinberg School of Medicine news release on an article that was published in the April 15, 2017 issue of Genes & Development
How do human cells repair a broken zipper of DNA?
A National Institute of Genetics news release on an article that was published in the April 15, 2017 issue of Genes & Development
How do human cells repair a broken zipper of DNA?
Dr. Toyoaki Natsume and Prof. Masato Kanemaki at National Institute of Genetics, ROIS, together with the group led by Prof. Ian D. Hickson at University of Copenhagen, reported a new system to deal with failure in DNA replication.
New book on Ion Channels from Cold Spring Harbor Laboratory Press
Ion Channels, just published by Cold Spring Harbor Laboratory Press, provides state-of-the-art techniques for investigating ion channel properties and function, particularly in the nervous system.
Different requirement of functional telomeres in neural stem cells and terminally differentiated neurons
A Scripps Research Institute news release on an article that was published in the April 1, 2017 issue of Genes & Development
Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote
A Nagoya University press release on an article that was published in the March 15, 2017 issue of Genes & Development
A novel role of the Dna2 translocase function in DNA break resection
A Yale University School of Medicine news release on an article that was published in the March 1, 2017 issue of Genes & Development
The laboratory of Prof. Cejka (Institute for Research in Biomedicine) studies how cells repair damaged or broken DNA.
In a new paper published in Genes and Development on March 23rd, 2017, Dr. Cejka and colleagues describe the function of one of the proteins that is involved in the initial DNA end processing.
Pancreatic α- and β-cellular clocks have distinct molecular properties and impact on islet hormone secretion and gene expression
A University of Geneva press release on an article that was published in the February 15, 2017 issue of Genes & Development
Phenotypic diversity and genotypic flexibility of Burkholderia cenocepacia during long-term chronic infection of cystic fibrosis lungs
In a study published today in Genome Research, scientists sequenced and phenotyped multiple B. cenocepacia isolates from 16 CF patients. They found extensive variation among isolates during chronic lung infection as well as changes in clinically relevant bacterial phenotypes.
Single tumor-initiating cells evade immune clearance by recruiting type II macrophages
A Life Sciences Institute, Zhejiang University news release on an article that was published in the February 1, 2017 issue of Genes & Development
Natural variation and dosage of the HEI10 meiotic E3 ligase control Arabidopsis crossover recombination
Researchers have discovered a key gene that influences genetic recombination during sexual reproduction in wild plant populations. Adding extra copies of this gene resulted in a massive boost to recombination and diversity in plant offspring. This finding could enable plant breeders to unlock crop variation, improve harvests and help ensure future food security.
New book on malaria from Cold Spring Harbor Laboratory Press
A renewed global effort in malaria control and elimination over the past decade has led to a substantial increase in basic research and significant advances in our understanding of the mechanisms underlying this complex disease. However, it is not always clear how such gains in knowledge will translate into improved strategies for the fight against malaria.
A genetic interaction analysis identifies cancer drivers that modify EGFR dependency
A Brigham and Women's Hospital research brief on an article that was published in the January 15, 2017 issue of Genes & Development
The PIDDosome activates p53 in response to supernumerary centrosomes
In a recent research work, the research team around Andreas Villunger from the Biozentrum Innsbruck is investigating a new activation mechanism of the tumor suppressor p53 after defective cytokinesis.
The Human Initiator is a Distinct and Abundant Element that is Precisely Positioned in Focused Core Promoters
UC San Diego Biologists Unlock Code Regulating Most Human Genes
PDX1 dynamically regulates pancreatic ductal adenocarcinoma initiation and maintenance
New research finds the protein PDX1 is critical for cancer growth, but blocking it may lead to more aggressive tumors.
Translation reprogramming is an evolutionarily conserved driver of phenotypic plasticity and therapeutic resistance in melanoma
Ludwig study reveals why cancer cells spread within the body Findings uncover an ancient mechanism that makes cancer cells invasive, explains melanoma's resistance to therapy and opens the door to development of novel cancer therapies
Substrate-induced Protein Splicing of a Recombinase Intein by Single-Stranded DNA
UAlbany Researchers Discover New Form of Protein Regulation
Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal
Out of gas and low on sperm? Kyoto University uncovers a genetic key to self-renewal of reproductive cells
The RNA-induced transcriptional silencing complex targets chromatin exclusively via interacting with nascent transcripts
Small RNAs interact with newly synthesized transcripts to silence chromatin
A phosphorylation-deubiquitination cascade regulates the BRCA2-Rad51 axis in homologous recombination
Enzyme that regulates DNA repair may offer new precision treatments for breast and ovarian cancer
New book on The Cytoskeleton from Cold Spring Harbor Laboratory Press
The cytoskeleton is the intracellular filament system that controls the morphology of a cell, allows it to move, and provides trafficking routes for intracellular transport.
New book on Cilia from Cold Spring Harbor Laboratory Press
Nearly every cell in the human body has one or more protrusive structures called cilia or flagella.
How to Turn White Fat Brown
Penn scientists discover a molecular trigger of fat-cell "browning" program, which could lead to better treatments for obesity and diabetes.
What happens in the cell nucleus after fertilization
A team of scientists at the Helmholtz Zentrum Mnchen shows changes in the immediate environment of DNA after the ovum and sperm fuse to form the zygote. The results suggest why all conceivable somatic cells can develop from the germ cells. The study has been published in the journal 'Genes and Development'.
Researchers find chemical tag that locks chromosomes together during meiosis
A Harvard Medical School news release on an article published in the November 1, 2016 issue of Genes & Development
Biophysics reveals the assembly mechanism of snRNP, a core component of the RNA splice complex
A Chinese Academy of Sciences press release on an article published in the November 1, 2016 issue of Genes & Development
Coupled enhancer and coding sequence evolution of a homeobox gene shaped leaf diversity
Millions of years ago, some plants in the mustard family made the switch from simple leaves to complex leaves through two tiny tweaks to a single gene. One tweak to a small enhancer sequence gave the gene a new domain of expression in the leaf. Paradoxically, the other tweak suboptimised its function in this new domain. But together, these changes gave rise to fit plants with complex leaves.
LMU researchers have demonstrated how deregulation of an epigenetic mechanism that is active only in the early phases of neurogenesis triggers the subsequent death of neural cells.
A Ludwig Maximilians University (LMU) press release on an article published in the October 1, 2016 issue of Genes & Development
Strain-level genetic differences within bacterial species yield new insights into mother-infant microbiomes
In a study published in Genome Research, scientists developed a new tool to examine genetic differences within bacterial species and uncover novel transmission patterns in mother-infant microbiomes and marine metagenomes not previously appreciated by species-level analyses.
An integrated metagenomics pipeline for strain profiling reveals novel patterns of bacterial transmission and biogeography
In a study published online today in Genome Research, scientists developed a new tool to examine genetic differences within bacterial species and uncover novel transmission patterns in mother-infant microbiomes and marine metagenomes not previously appreciated by species-level analyses
Nucleoporin-mediated regulation of cell identity genes
Heart disease, leukemia linked to dysfunction in nucleus... Salk scientists identify the cell nucleus as a driver of gene expression and, sometimes, disease
Neuron-type specific miRNA represses two broadly expressed genes to modulate an avoidance behavior in C. elegans
A Research Institute of Molecular Pathology (IMP) press release on an article published in the September 15, 2016 issue of Genes & Development
Temperature regulates splicing efficiency of the cold-inducible RNA-binding protein gene Cirbp
A University of Geneva press release on an article published in the September 1, 2016 issue of Genes & Development
Polarized regulatory landscape and Wnt responsiveness underlie Hox activation in embryos
A Hubrecht Institute press release on an article published in the September 1, 2016 issue of Genes & Development
Direct interrogation of the role of H3K9 in metazoan heterochromatin function
A UNC Health Care/UNC School of Medicine press release on an article published in the August 15, 2016 issue of Genes & Development
Unbiased identification of signal-activated transcription factors by Barcoded Synthetic TAndem Repeat Promoter screening (BC-STAR-PROM)
A University of Geneva press release on an article published in the August 15, 2016 issue of Genes & Development
New manual on Fission Yeast from Cold Spring Harbor Laboratory Press
Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few.
New book on Antibiotics and Antibiotic Resistance from CSHLPress
One of the greatest medical accomplishments of the past century was the introduction of antibiotics into the clinic. However, the use of these lifesaving drugs rapidly led to the emergence of antibiotic-resistant pathogens, which have become increasingly difficult and expensive to eradicate.
Autophagy Provides Metabolic Substrates to Maintain Energy Charge and Nucleotide Pools in Ras-Driven Lung Cancer Cells
Cutting Off the Cancer Fuel Supply -- Research from investigators at Rutgers Cancer Institute of New Jersey and Princeton University has identified a new approach to cancer therapy in cutting off a cancer cell’s ‘fuel supply’ by targeting a cellular survival mechanism known as autophagy.
The 80th CSH Symposium Proceedings addresses 21st Century Genetics: Genes at Work
The 80th Cold Spring Harbor Symposium was held to mark the 150th anniversary of Gregor Mendel’s landmark 1865 presentation of his paper "Experiments on Plant Hybridization", which laid the groundwork for modern genetics.
HNF6 and Rev-erb-alpha integrate hepatic lipid metabolism by overlapping and distinct transcriptional mechanisms
Penn study sheds light on biology of leading cause of liver failure -- Codependence of Cell Nucleus Proteins Key to Understanding Fatty Liver Disease
A microRNA program in the C. elegans hypodermis couples to intestinal mTORC2/PQM-1 signaling to modulate fat transport
A Massachusetts General Hospital (MGH) research team reports finding that a previously unknown interaction between metabolic pathways in two different tissues within the C. elegans roundworm triggers a key step in maturation. In their report published in Genes & Development, the investigators describe finding how microRNAs in the skin of the roundworm, which are known to control the animals' transition through key developmental stages, link to a signaling pathway that directs the transport of lipids from storage in the intestine to reproductive cells for support of embryonic growth.
Modeling invasive lobular breast carcinoma by CRISPR/Cas9-mediated somatic genome editing of the mammary gland
Annunziato et al. describe a novel strategy for in vivo validation of candidate tumor suppressors implicated in invasive lobular breast carcinoma (ILC). Whereas intraductal injection of Cas9-encoding lentiviruses induced Cas9-specific immune responses and development of tumors that did not resemble ILC, lentiviral delivery of a Pten targeting sgRNA in mice with mammary gland-specific loss of E-cadherin and expression of Cas9 efficiently induced ILC development.
The adrenal capsule is a signalling centre controlling cell renewal and zonation through Rspo3
Adrenal glands are essential organs that maintain hormonal balance in the body. In this study we have identified Rspo3 as a gene that maintains adrenal function throughout life by controlling functional specification of cells within the adrenal and ensuring cell renewal of damaged cells.
Researchers at the Universit� libre de Bruxelles, ULB develop new techniques to assess the fate of stem cells in vivo.
Publication in Genes & Development: researchers at the Université libre de Bruxelles, ULB develop new techniques to assess the fate of stem cells in vivo.
New book on the p53 protein from Cold Spring Harbor Laboratory Press
Decades of research on the tumor suppressor p53 have revealed that it plays a significant role as a "guardian of the genome," protecting cells against genotoxic stress.
Venus flytrap exploits common plant defense strategies for carnivorous lifestyle
Venus flytraps have fascinated biologists for centuries, however, the molecular underpinnings of their carnivorous lifestyle remain largely unknown.
Single-cell analysis of embryos reveals mis-segregation of parental genomes
Single-cell embryos contain a set of maternal and paternal chromosomes, and as the embryo grows, daughter cells receive a copy of each.
Human genome evolution fast-tracked by mutations induced by anti-viral enzyme
Evolution is thought to proceed through the gradual accumulation of independent mutations in each new generation.
New book on budding yeast from Cold Spring Harbor Laboratory Press
Over the past century, studies of the budding yeast Saccharomyces cerevisiae have helped to unravel principles of nearly every aspect of eukaryotic cell biology--from metabolism and molecular genetics to cell division and differentiation.
New book on microbial evolution from Cold Spring Harbor Laboratory Press
Bacteria have been the dominant forms of life on Earth for the past 3.5 billion years. They rapidly evolve, constantly changing their genetic architecture through horizontal DNA transfer and other mechanisms. Consequently, it can be difficult to define individual species and determine how they are related.
bioRxiv preprints can now be submitted directly to leading research journals
New feature saves scientists time, propels preprints into mainstream of biomedical science publishing
New book on DNA recombination from Cold Spring Harbor Laboratory Press
Homologous recombination involves the precise exchange of similar or identical nucleic acid sequences between two DNA molecules.
New from CSHLPress, an indispensable bench-side handbook for biologists using R
Using R at the Bench: Step-by-Step Data Analytics for Biologists, published by Cold Spring Harbor Laboratory Press and available in spiral bound hardcover and e-book formats, is a convenient bench-side handbook for biologists, designed as a handy reference guide for elementary and intermediate statistical analyses using the free/public software package known as "R."
’The Road to Discovery: A Short History of Cold Spring Harbor Laboratory,’ just released
The Road to Discovery: A Short History of Cold Spring Harbor Laboratory was published this year to mark the 125th anniversary of Cold Spring Harbor Laboratory.
New book on neurogenesis from Cold Spring Harbor Laboratory Press
In discrete locations of the adult brain, new neurons are born from stem cells that differentiate, migrate, and integrate into the existing neural network. This process is implicated in normal brain functions such as memory formation and is disrupted in many disease states, including Alzheimer’s, schizophrenia, and depression.
Cold Spring Harbor Laboratory Press Launches New Journal of Precision Medicine
Cold Spring Harbor Molecular Case Studies presents genomic and molecular analyses of individuals or cohorts alongside their clinical presentations and phenotypic information.
New book on Cell Death Techniques from Cold Spring Harbor Laboratory Press
Regulated cell death, which is involved in nearly every aspect of animal development and physiology, can be challenging to study in the laboratory.
An essential guide to the genetic terms that impact your research, from CSHLPress
In Decoding the Language of Genetics, published by Cold Spring Harbor Laboratory Press, the distinguished geneticist David Botstein offers help and advice to scientists and physicians daunted by the arcane technical terms that flourish in his discipline.
New book on ’Size Control in Biology,’ from Cold Spring Harbor Laboratory Press
Size is a primary feature of living things. From the egg to adult, the various organs, tissues, cells, and subcellular structures that make up an organism achieve appropriate sizes so that they effectively fit and function together. The misregulation of this growth can lead to diseases such as cancer.
New book on ’Molecular Approaches to Reproductive and Newborn Medicine’ from CSHLPress
The development of an infant, from conception to the postnatal period, involves the dynamic coordination of numerous biological factors in the mother, father, and fetus.
New book on cognition from Cold Spring Harbor Laboratory Press
Cognition, the Proceedings of the Cold Spring Harbor 79th Symposia on Quantitative Biology, captures many of the tremendous discoveries currently being made by neuroscientists and psychologists working on cognitive processes at scales varying from the molecular to circuit to whole-brain and theoretical studies.
Learn cutting-edge research opportunities using new DNA sequencing technologies
Next-generation DNA sequencing (NGS) technology has revolutionized biomedical research, making genome and RNA sequencing an affordable and frequently used tool for a wide variety of research applications...
New Book On Hepatitis B and Delta Virus from CSHLPress
Despite the availability of an effective vaccine for hepatitis B, hundreds of millions of people worldwide are infected with the hepatitis B virus (HBV). This virus can cause serious liver damage and cancer in chronically infected patients. Hepatitis delta virus (HDV), a satellite of HBV, can exacerbate the disease.
’Epigenetics:’ The new comprehensive guide from Cold Spring Harbor Laboratory Press
In many biological processes the regulation of gene expression involves epigenetic mechanisms.
New book on mitosis from Cold Spring Harbor Laboratory Press
Virtually every cell in a living organism contains an identical set of chromosomes thanks to mitosis, a complex process involving hundreds of proteins and regulatory steps that ensures duplicated chromosomes are segregated equally into each daughter cell when a cell divides.
New Book on ’Intellectual Property in Molecular Medicine’ from CSHLP
Patents are an important way of protecting inventions in the pharmaceutical and biotechnology industries. However, intellectual property law reforms have not kept pace with the rapid advances in genomics, synthetic biology, and stem cell research.
New book on glia from Cold Spring Harbor Laboratory Press
Glia are cells that serve to nourish and support the neuronal cells that relay electrical signals through the nervous system.
New book on retinal disorders from Cold Spring Harbor Laboratory Press
The retina is a layer of neural tissue that lines the inner eye and captures visual stimuli. Hereditary, physiologic, or environmental factors can lead to various retinal disorders and may cause blindness. The recent application of molecular genetic techniques to retinal disorders has led to remarkable advances in the understanding, diagnosis, and treatment of these conditions.
New book on human fungal pathogens from CSHLPress
Some yeasts and molds that are common in the environment can infect humans and cause a range of diseases, from superficial (e.g., athlete’s foot) to severe (e.g., cryptococcal meningitis).
New book on ’The Biology of Heart Disease’ from Cold Spring Harbor Laboratory Press
The heart is the first organ to form in a developing embryo, and all subsequent life processes depend on its proper function. But a range of genetic and environmental factors can lead to its failure. Inherited mutations give rise to congenital heart disease, the most common birth defect, and abnormalities of the adult heart are a leading cause of illness and death in industrialized countries.
New book on ’Innate Immunity and Inflammation’ from Cold Spring Harbor Laboratory Press
The innate immune system is rapidly activated in response to infection and injury. It is a generic rather than pathogen-specific response that recruits immune cells, promotes inflammation, and mobilizes the adaptive immune system. Excessive or chronic inflammation may cause tissue damage, so a careful balance is required to restore homeostasis.
New book from Cold Spring Harbor Laboratory Press: Tuberculosis
Tuberculosis (TB) is a serious infectious disease of the lungs that is usually caused by the bacterium Mycobacterium tuberculosis. Nearly one-third of the world’s population is currently infected with latent TB, and millions of individuals develop the active, potentially fatal form of the disease each year.
New book from CSHL Press introduces the modern view of metabolic studies
In a refreshing approach to the subject of metabolism, Navigating Metabolism provides a conceptual framework for navigating complex metabolic pathways, showing how cells generate energy and synthesize cellular constituents and then further relating metabolic reactions to molecular, genetic, and signaling underpinnings.
New book from CSHL Press with focus on genetics, personalized medicine and human diversity
Human Variation from Cold Spring Harbor Laboratory Press provides a state-of-the-art view of human genetic variation and what we can infer from it, surveying the genetic diversity seen in Africa, Europe, the Americas, and India.
From the renowned Cold Spring Harbor Laboratory course
Molecular Neuroscience: A Laboratory Manual serves as a comprehensive practical guide to molecular and cellular methods for neuroscientists.
New book from CSHL Press explores the evolution of sexual reproduction
Written and edited by experts in the field, The Genetics and Biology of Sexual Conflict from Cold Spring Harbor Perspectives in Biology examines the underlying biology of sexual conflict—from the molecular to the behavioral levels—and its role as an important driver of evolution.
New book from CSHLPress provides a solution for ’informatics anxiety’
Informatics can vastly assist progress in research and development in cell and molecular biology and biomedicine.
From the basics to the cutting edge of molecular biology and genomics in one small book
As biology becomes more quantitative and computational, increasing numbers of physical scientists, mathematicians, and engineers are moving into areas such as genomics, developmental biology, neuroscience, and systems biology.
African elephant genome suggests they are superior smellers
In a study published today in Genome Research, researchers examined the olfactory receptor (OR) repertoire encoded in 13 mammalian species and found that African elephants have the largest number of OR genes ever characterized; more than twice that found in dogs, and five times more than in humans.
New book from CSHLPress introduces the fundamental principles of signal transduction
Cells must respond to a wide variety of signals. These include hormones, growth factors, morphogens, and environmental stress, as well as signals from internal regulators and checkpoints. A complex network of signal transduction pathways within the cell ensures that these signals are relayed to the correct molecular targets and that the cell responds appropriately.
New book from CSHLPress covers all aspects of MYC biology
The MYC gene family plays essential roles in normal development and in multiple cellular functions.
New book from CSHLPress provides a comprehensive review of the biology of the skin
The skin is the largest organ in the human body, and it is constantly bombarded with external stimuli. It offers protection and insulation, prevents dehydration, and senses the environment.
New book by James Watson: Perfect for anyone who has ever learned something from a father
NEW YORK – JUNE 5, 2014 – Cold Spring Harbor Laboratory Press is pleased to announce the publication of Father to Son: Truth, Reason, and Decency by James D. Watson. The book is an engaging work that chronicles Watson’s lineage through thought-provoking reflections, memorabilia, and imagery.
New book, Origin and Evolution of Eukaryotes, from Cold Spring Harbor Laboratory Press
All protists, fungi, animals, and plants on Earth are eukaryotes. Their cells possess membrane-bound organelles including a nucleus and mitochondria, distinct cytoskeletal features, and a unique chromosome structure that permits them to undergo mitosis or meiosis. The emergence of eukaryotic cells from prokaryotic ancestors about 2 billion years ago was a pivotal evolutionary transition in the history of life on Earth. But the change was abrupt, and few clues exist as to the nature of the intermediate stages.
New handbook is an essential guide for scientists venturing into biomedical consulting
Cold Spring Harbor Laboratory Press is pleased to announce the publication of Connecting with Companies: A Guide to Consulting Agreements for Biomedical Scientists by Edward Klees, J.D. and H. Robert Horvitz, Ph.D. The book is an essential resource for academic scientists and physicians considering consulting work in biomedicine.
Genome sequencing of MRSA infection predicts disease severity
In a study published online today in Genome Research, researchers used the genome sequence of MRSA to predict which isolates were highly toxic, thus potentially personalizing the treatment of individual MRSA infections.
Early brain development implicated in Restless Legs Syndrome
Restless Legs Syndrome (RLS), a neurological disorder characterized by unpleasant sensations in the legs and the urge move them, affects up to 10% of Americans, although little is known about its cause.
New book on endocytosis from Cold Spring Harbor Laboratory Press
During endocytosis, extracellular molecules and plasma membrane components are selectively internalized by cells. This fundamental process of "cellular ingestion" is required for diverse activities such as nutrient uptake, cell adhesion and migration, signal transduction, cytokinesis, neurotransmission, and antigen presentation.
New book on mouse models of cancer from Cold Spring Harbor Laboratory Press
The laboratory mouse is an important model for addressing questions in cancer biology. In recent years, the questions have become more refined, and mouse models are increasingly being used to develop and test cancer therapeutics. Thus, the need for more sophisticated and clinically relevant mouse models has grown, as has the need for innovative tools to analyze and validate them.
New book on calcium techniques from Cold Spring Harbor Laboratory Press
Life begins with a surge of calcium ions (Ca2+) at fertilization, and thereafter, Ca2+ signaling influences nearly every aspect of mammalian development and physiology, from gene expression and cell proliferation to muscle contraction and nerve impulses.
New book on calcium techniques from Cold Spring Harbor Laboratory Press
Life begins with a surge of calcium ions (Ca2+) at fertilization, and thereafter, Ca2+ signaling influences nearly every aspect of mammalian development and physiology, from gene expression and cell proliferation to muscle contraction and nerve impulses.
New book from Cold Spring Harbor Laboratory Press on transplantation
The transplantation of organs such as the heart, kidney, and lungs is an important means of replacing seriously damaged or diseased body parts. However, a transplanted organ may fail if the recipient’s immune system mounts a response to it.
New book on ’Signaling by Receptor Tyrosine Kinases’ by Cold Spring Harbor Laboratory Press
Receptor tyrosine kinases are a large family of cell-surface receptors that respond to a variety of intercellular signals, including insulin, growth factors such as epidermal growth factor (EGF) and fibroblast growth factor (FGF), and molecules involved in neuronal guidance.
New book on mitochondria from Cold Spring Harbor Laboratory Press
Mitochondria are intracellular organelles that power the cell by metabolizing glucose and other energy sources to generate ATP.
Much of what is known about cancer development comes from curiosity-driven, basic science
In a new book Blue Skies and Bench Space, published by Cold Spring Harbor Laboratory Press and written with the assistance of the past and present inhabitants of the London Research Institute, Kathy Weston tells the inside story of the lab’s greatest voyages into the scientific unknown, revealing the personalities behind the dry logic and passive voice of the scientific paper.
Human immune system shapes skin microbiome
Our skin plays host to millions of beneficial and potentially disease-causing microorganisms; however, whether our immune system influences these microbial communities to prevent disease is unknown.
New manual from Cold Spring Harbor Laboratory Press on culturing neural cells
Cell culture systems for specific neural cell types are essential for studies of their development and function.
New book from CSHL Press on DNA repair and responses to DNA damage
Cellular DNA is constantly bombarded with environmental and chemical assaults that damage its molecular structure. In addition, the normal process of DNA replication is prone to error and may introduce mutations that can be passed to daughter cells. If left unrepaired, these DNA lesions can have serious consequences, such as cancer.
New edition of classic manual provides insights into development and use of monoclonal antibodies
The now classic lab manual Antibodies, by Harlow and Lane, has been revised, extended, and updated by Edward Greenfield of the Dana-Farber Cancer Center, with contributions from other leaders in the field.
New book reviews the biology of synapses
Synapses are bulbous structures where two neurons communicate. Neurotransmitter molecules released from the presynaptic terminal of one neuron diffuse to the postsynaptic terminal on the other, binding to receptors that lead to propagation or modulation of the signal
Bridges experimental and bioinformatics perspectives to delineate protein-DNA interactions
One of the foundations of molecular biology is how the interactions of proteins with DNA control many aspects of gene expression.
Essential informatics methods and tools for analyzing the explosion of NGS data
Next-generation DNA sequencing (NGS) technology has revolutionized biomedical research, making complete genome sequencing an affordable and frequently used tool for a wide variety of research applications. Bioinformatics methods to support DNA sequencing have become a critical bottleneck for many researchers and organizations wishing to make use of NGS technology.
Protein Synthesis and Translational Control
Introducing a new book on the synthesis of proteins Now available for purchase
Molecular Cloning: A Laboratory Manual, Fourth Edition
Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions.
Protein Homeostasis covers the entire spectrum of protein homeostasis
Written and edited by experts in the field, Protein Homeostasis covers the entire spectrum of protein homeostasis in healthy cells and the diseases that result when control of protein production, protein folding, and protein degradation goes awry.
Introducing three new titles from Cold Spring Harbor Laboratory Press
Introducing three new titles from Cold Spring Harbor Laboratory Press
Eukaryotic cells AND Nuclear receptors
Eukaryotic cells respond to nutrient deprivation by halting the energetically demanding processes of ribosome biosynthesis, and redirecting resources to the translation of other, essential genes.--AND -- Nuclear receptors are well known as ligand-regulated transcription factors.
The expansion of stem cell populations AND A large part of the human genome codes for noncoding RNA (ncRNA).
Perry et al. investigated how the Wnt/β-cateninand PTEN/PI3K/Akt pathways cooperate in hematopoietic stem cells (HSC) to regulate self-renewal and expansion. -- AND -- Cabili et al. present the first comprehensive map of large intergenic noncoding RNAs (lincRNAs), providing insight into the characteristics and properties of these RNAs.
Transcriptional Regulation of Xenobiotic Detoxification in Drosophila
The activation of xenobiotic metabolism in insects underlies insecticide detoxification, and, ultimately, acquired pesticide resistance, which has enormous agricultural and public health consequences. Misra et al. show that Nrf2 pathway activation is sufficient to confer resistance to the common and general-use pesticide, malathion.
Viruses in the human gut show dynamic response to diet
August 31, 2011 - The digestive system is home to a myriad of viruses, but how they are involved in health and disease is poorly understood.
A novel mechanism for the transcriptional regulation of Wnt signaling in development
Two mutually antagonistic sets of morphogens – Sonic hedgehog (Shh) and Wnt proteins – establish dorsal/ventral polarity in the embryonic eye and forebrain.
Study reveals new link between Alzheimer’s disease and healthy aging
August 16, 2011 - Alzheimer’s disease and frontotemporal lobar degeneration (FTLD) are two of the most prevalent forms of neurodegenerative disorders.
The Mammary Gland as an Experimental Model
Studies of mammary gland biology are essential in the fight against breast cancer. As described in a new book from Cold Spring Harbor Laboratory Press, The Mammary Gland as an Experimental Model, this organ also represents an excellent model system for investigation of physiological and pathological processes that occur throughout the body.
The Mammary Gland as an Experimental Model
COLD SPRING HARBOR, N.Y. (August 15, 2011) - Studies of mammary gland biology are essential in the fight against breast cancer. As described in a new book from Cold Spring Harbor Laboratory Press, The Mammary Gland as an Experimental Model, this organ also represents an excellent model system for investigation of physiological and pathological processes that occur throughout the body.
Illuminating life: how RNA, after a century in the shadows, emerged into the spotlight
RNA is central to the molecular basis of life and to the origin of life itself. While its study has captivated the current generation of molecular biologists, many do not know the historical underpinnings of the field.
The Biology of Lipids: Trafficking, Regulation, and Function
As the major structural component of cell membranes, lipids not only serve as barriers but also play active roles in cellular function. The fission and fusion of lipid membranes underlies the majority of protein trafficking in cells.
Nitric oxide coordinates metabolism, growth and development via the Nuclear Receptor E75
In this paper, Caceres et al. reveal that the E75/Rev-erb family of nuclear receptors mediates nitric oxide (NO)signaling in the nucleus, and its regulation of development and metabolism. In another paper, 129 years after mitotic chromosomes were first described by Walther Flemming, Shintomi and Hirano finally reveal what determines their shape.
The first studies utilizing the Collaborative Cross mice are published in Genome Research
July 14, 2011 - The Collaborative Cross (CC) represents a large collection of new inbred mouse strains created by the mouse genetics community aimed at revolutionizing the study of complex genetic traits and diseases. strains and wild-derived strains, the CC captures nearly 90% of known genetic variation in laboratory mice, far surpassing more commonly used inbred strains.
Fungus farming ant genome reveals insight into adaptation of social behavior
June 30, 2011 - The development of agriculture was a significant event in human cultural evolution, but we are not the only organisms to have adopted an agricultural way of life. In a study published online today in Genome Research (, researchers have sequenced the genome of a fungus farming leaf-cutting ant, revealing new insights into the genetics and molecular biology behind this unusual lifestyle.
Semaphorin3E-Plexin-D1 signaling regulates VEGF function in developmental angiogenesis via a feedback mechanism
In this paper, Kim et al. reveal a novel mechanism by which the traditional axon guidance ligand-receptor guidance cues, Semaphorin 3E and Plexin-D1, directangiogenesis. In another paper, Kang et al. reveal that a single post-translational modification on histone H4 differentially regulates these distinct nucleosome assembly pathways.
Cutting-edge imaging techniques for neuroscientists available in third volume of laboratory manual series
COLD SPRING HARBOR, N.Y. (June 13, 2011) - Neuroscientists have long pioneered the use of new visualization techniques. Imaging in Neuroscience: A Laboratory Manual continues that tradition by presenting an outstanding collection of methods for visualizing the nervous system. It is the third volume in a series of imaging manuals published by Cold Spring Harbor Laboratory Press.
Noted science historian Elof Carlson traces how the idea of mutation has changed in six generations
Today, most scientists use the term "mutation" to describe a change in an individual gene - more precisely a minute alteration of its DNA. But the term has also achieved a powerful presence in popular culture, to describe a process by which individuals gain exceptional, often malign, characteristics.
An eloquent and inspirational account of a man’s struggle to live with courage in the face of a fatal illness, reminding us that understanding cancer remains an urgent goal in biomedicine
In March 2009, during a routine medical exam, Charles Harris learned that he had incurable cancer. Although a private man, he began a blog to keep family and friends current on his progress, and found a wider audience. From the blog was born the new book Incurable, an account of a man’s struggle to live vibrantly and with courage in the face of a fatal illness.
New book on intermediary metabolism reveals intriguing complexity
COLD SPRING HARBOR, N.Y. (May 11, 2011) "Metabolism is Not Boring!" asserts the introduction to a recent special issue of Science (Vol 330, 3 December 2010). On the contrary, the ways in which cells obtain energy, use external nutrients, and assemble the building blocks of macromolecules are crucial for life. And the basics of these processes - intermediary metabolism - are similar from the single-celled to multicellular organisms.
New book on animal behavior provides integrated view
COLD SPRING HARBOR, N.Y. (March 22, 2011) - An animal’s behavior is probably its most attention-attracting aspect, both to scientists and nonscientists alike. Its behavior involves processes internal to the animal - genetics, neurobiology, and physiology - as well as those external to it - environment and social surroundings.
Gene fusion mechanisms offer new clues to origin of pediatric brain tumors
March 10, 2011 - A detailed analysis of gene fusions present at high frequency in the most common pediatric brain tumors has been performed for the first time in a study published online today in Genome Research (, shedding new light on how these genomic rearrangements form in the early stages of cancer.
Entire T-cell receptor repertoire sequenced revealing extensive and unshared diversity
February 24, 2011 - T-cell receptor diversity in blood samples from healthy individuals has been extensively cataloged for the first time in a study published online today in Genome Research, setting the stage for a better understanding of infectious disease, cancer, and immune system disorders.
Microproteomics, Immunoimaging featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y. (Tues., Feb. 1, 2011)- Comparative and quantitative proteomic technologies have not progressed to the extent of their genomic and transcriptomic counterparts. Unlike the genome, which is essentially identical in the somatic cells of a given organism, the proteome varies in different cells, and there is no self-replicating amplification mechanism for proteins such as the polymerase chain reaction (PCR) for DNA. Because of this, methods that extract, separate, detect and identify proteins from extremely small samples are needed.
Genetic archaeology finds parts of our genome more closely related to orangutans than chimps
January 26, 2011 – In a study published online today in Genome Research (, in coordination with the publication of the orangutan genome sequence, scientists have presented the surprising finding that although orangutans and humans are more distantly related, some regions of our genomes are more alike than those of our closest living relative, the chimpanzee.
New user-friendly resource connects human genes to biological functions
COLD SPRING HARBOR, N.Y. (Jan. 11, 2011) – The human genome sequence, initially completed in draft form nearly a decade ago, has revolutionized biological research.
New book highlights the cellular and molecular determinants of brain wiring
COLD SPRING HARBOR, N.Y. (Jan. 6, 2011) - During the development of the nervous system, precise and complex connections are made among neurons. These connections are determined by external molecular cues that influence the direction in which neurons grow.
Key plant hormone and its roles in plant biology is focus of new book
COLD SPRING HARBOR, N.Y. (Jan. 5, 2011) - Auxin is a critical hormone in plants, playing a key role in nearly all aspects of plant development and physiology.
Developmental biology is focus of new volume in laboratory manual series on imaging
COLD SPRING HARBOR, N.Y (Jan. 4, 2011) - Imaging technologies have revolutionized the study of developmental biology and are now essential tools for researchers in the field.
Transcriptome analysis, organ culture methods featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y. (Mon., Jan. 3, 2011) – New technologies and methods are spurring a renaissance in the study of organogenesis. Organogenesis, essentially the process through which a group of cells becomes a functioning organ, has important connections to biological processes at the cellular and developmental levels, and its study offers great potential for medical treatments through tissue engineering approaches.
Ovarian cancer clue: Methylation-mediated suppression of a key pathway is found
December 14, 2010 - Ovarian cancer is the leading cause of death among gynecological cancers. To better understand the disease and improve therapies, researchers are investigating how deregulation of genes across the genome could be contributing to malignancy.
Tracing microbes between individuals towards personalized oral health care
December 13, 2010 - The human body is home to a complex ecosystem of microbes increasingly recognized as having a critical role in both health and disease. Viruses can attack and change the composition of bacterial communities, yet little is known about how this might influence human health.
Authoritative laboratory manual on RNA methods is released
COLD SPRING HARBOR, N.Y. (Dec. 7, 2010) - RNA’s key role in critical cellular functions has made it a powerful tool for unraveling biological processes.
Generating form and function: Imaging development featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR,N.Y. (Wed., Dec. 1, 2010) - New imaging technologies have revolutionized the study of developmental biology. Where researchers once struggled to connect events at static timepoints, imaging tools now offer the ability to visualize the dynamic form and function of molecules, cells, tissues, and whole embryos throughout the entire developmental process.
New book reviews the features and functions of the cell nucleus
COLD SPRING HARBOR,N.Y.(Nov. 30, 2010) - The nucleus of a cell contains its DNA and is the site where DNA replication, transcription, and RNA processing take place. Within the nucleus, nuclear bodies appear to dynamically self-organize, assembling and disassembling according to the functional demands of the cell.
In new novel, secret discovery threatens company, damages love affair, and undermines science as search for truth
COLD SPRING HARBOR, N.Y. (Nov. 29, 2010) - Research scientist Jennifer Rohn’s newly released novel, The Honest Look, set in a start-up biotechnology company, explores conflicts between the need for scientific truth and the drive for commercial success.
Remarkable biological complexity of bacterial cells is focus of newly released book
COLD SPRING HARBOR, N.Y. (Nov. 24, 2010)- The bacterial cell is often thought of as a "bag of enzymes" - a single compartment with limited internal structure, a primitive cell cycle, and relatively simple metabolism.
New book reviews mechanisms of T-cell and B-cell activation
COLD SPRING HARBOR, N.Y. (Nov. 23, 2010) Specialized white blood cells called T cells and B cells are critical for immunity - helping the body to identify and eliminate "non-self" substances such as viruses and bacteria. The activation of T cells and B cells occurs when immunoreceptors on the cell surface bind to specific regions on, or derived from, the invaders.
First volume of new laboratory manual series on imaging is released
COLD SPRING HARBOR, N.Y. (Nov. 18, 2010) Sophisticated techniques that permit the visualization of dynamic processes in cells, tissues, and organ systems at extraordinary levels of resolution have become tremendously valuable in biological research. However, finding the right imaging method and optimizing it for data collection can be a daunting process, even for an established imaging laboratory.
Recombination hotspot stacks the DNA deck in finding a new diabetes susceptibility gene
The autoimmune disease type 1 diabetes (T1D), also known as juvenile diabetes, is diagnosed in approximately 70,000 children worldwide per year. Genetics is increasingly being recognized as playing a significant role in susceptibility to the disorder, but outside a handful of genes, a clear understanding of the genetic architecture that underlies T1D has remained elusive.
Means to an End: Apoptosis and Other Cell Death Mechanisms
COLD SPRING HARBOR, N.Y. (Nov. 2, 2010) - One million cells in our bodies die every second - they commit suicide by a mechanism known as apoptosis.
Imaging in depth: Three-dimensional microscopy featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y.(Mon., Nov. 1, 2010) - Imaging has rapidly become a defining tool of the current era in biological research. But finding the right method and optimizing it for data collection can be a daunting process, even for an established imaging laboratory.
Radiation pharmacogenomics identifies biomarkers that could personalize cancer treatment
October 5, 2010 - Radiation therapy is used to treat more than half of all cancer cases, but patient response to therapy can vary greatly. Genetics is increasingly being recognized as a significant contributor to inter-individual response to radiation, but the biology underlying response remains poorly understood.
Methylation of chromatin and noncoding RNA featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y. (Fri., Oct. 1, 2010)- Post-translational modifications of histones play an important role in regulating chromatin dynamics and function. One such modification, methylation, is involved in the regulation of the epigenetic program of a cell, determining chromatin structure, and regulating transcription.
New book reviews ancient and modern worlds of RNA
OLD SPRING HARBOR, N.Y. (Sept. 28, 2010) - A new book, RNA Worlds: From Life’s Origins to Diversity in Gene Regulation, reviews our understanding of two RNA worlds: the primordial RNA world before DNA, in which RNA was both information store and biocatalyst; and the contemporary RNA world, in which mRNA, tRNA, rRNA, siRNA, miRNA, and a host of other RNAs operate.
Biography captures Sydney Brenner’s unflagging scientific curiosity and lively personality
COLD SPRING HARBOR, N.Y. (Sept. 22, 2010) - From helping to decipher the genetic code to establishing the worm C. elegans as a model organism, and from directing the Laboratory of Molecular Biology in Cambridge to advising research institutes around the world, Nobel Prize winner Sydney Brenner has had a long and impressive career.
Interviews bring genetics to life in new book
COLD SPRING HARBOR, N.Y. (Sept. 16, 2010) - A new book, Speaking of Genetics: A Collection of Interviews, contains 22 interviews of prominent individuals in the field of genetics.
Drug-resistant malaria suggests a health policy change for pregnant women and infants
September 9, 2010 - Malaria remains a serious global health problem, killing more than one million people per year. Treatment of the mosquito-borne illness relies on antibiotics, and the emergence of drug-resistant malaria is of growing concern.
Cold Spring Harbor Protocols features chromosomal rearrangement, gene copy number methods
COLD SPRING HARBOR, N.Y. (Wed., Sept. 1, 2010) A cell devotes a significant amount of effort to maintaining the stability of its genome, preventing the sorts of chromosomal rearrangements characteristic of many cancers. Assays that measure the rate of gross chromosomal rearrangements (GCRs) are needed in order to understand the individual genes and the different pathways that suppress genomic instability.
Reshaping the gut microbiome could herald new treatments for bowel diseases
August 24, 2010 - Home to a diverse range of microorganisms, a healthy human body contains at least tenfold more bacteria cells than human cells. The most abundant and diverse microbial community resides in the intestine, and changes to the gut microbiota are linked with diseases such as ulcerative colitis and Crohn’s disease.
Vitamin D linked to autoimmune and cancer disease genes, underscoring risks of deficiency
August 24, 2010 - Vitamin D insufficiency is a risk factor for a number of diseases and thus, is a growing concern worldwide, as approximately one billion people may be vitamin D deficient. However, the biological basis for vitamin D deficiency predisposing to disease is poorly understood.
Genetic clue to chronic pain could lead to new treatments for the condition
Chronic pain is a serious medical problem, afflicting approximately 20% of adults. Some individuals are more susceptible than others, and the basis for this remains largely unknown. In a report published online today in Genome Research, researchers have identified a gene associated with susceptibility to chronic pain in humans, signaling a significant step toward better understanding and treating the condition.
New book describes career opportunities in clinical research and how to qualify for them
For those with an aptitude in science and an interest in working with medical discovery teams, careers at drug, medical device, and contract research organization (CRO) companies can be fulfilling. A new book from Cold Spring Harbor Laboratory Press, Career Opportunities in Clinical Drug Research, introduces readers to entry-level clinical job opportunities and explains how to qualify for them.
’Runaway’ development implicated in loss of function of the aging brain
July 20, 2010 - The brain undergoes rapid growth and development in the early years of life and then degenerates as we progress into old age, yet little is known about the biological processes that distinguish brain development and aging.
Prostate cancer risk variant found to be in a functional DNA sequence linked with disease
July 13, 2010 - Recent genetic association studies have uncovered a number of DNA variants associated with prostate cancer. However, some of these risk variants lie outside of genes, posing a challenge to researchers working to understand the biology of cancer.
World-renowned authorities provide current perspectives on Darwin’s theory of evolution
In new book, contributors describe the molecular basis of evolution as well as the relevance of evolutionary theory for sociology, culture, and the economy
Cold Spring Harbor Protocols Features Drosophila Neurobiology Methods
Since the early days of the 20th century and Thomas Hunt Morgan’s famous "Fly Room" at Columbia University, the fruit fly Drosophila melanogaster has been at the forefront of biological research.
New book provides summary of research into the origins of life on Earth
COLD SPRING HARBOR, N.Y.(June 29, 2010) Life arose on Earth more than three billion years ago. How the first self-replicating systems emerged from prebiotic chemistry and evolved into primitive cell-like entities is an area of intense research, spanning molecular and cellular biology, organic chemistry, cosmology, geology, and atmospheric science.
Mitochondrial genome analysis revises view of the initial peopling of North America
June 29, 2010 - The initial peopling of North America from Asia occurred approximately 15,000-18,000 years ago, however estimations of the genetic diversity of the first settlers have remained inaccurate.
New book reviews research on p53 the guardian of the genome and its relatives
A new book from Cold Spring Harbor Laboratory Press, The p53 Family, provides a comprehensive review of the functions of the p53 family. It was edited by Arnold Levine and David Lane, who independently discovered p53 about 30 years ago.
Electrophoresis of RNA, Avian Imaging featured in June’s Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y. (Tues., June 1, 2010) – Gel electrophoresis is one of the most important and frequently used techniques in RNA analysis. Electrophoresis is used for RNA detection, quantification, purification by size and quality assessment.
New book reviews research on key signaling molecule, NF-kB
COLD SPRING HARBOR, N.Y. (May 25, 2010) - A new book from Cold Spring Harbor Laboratory Press, NF-κB: A Network Hub Controlling Immunity, Inflammation, and Cancer, summarizes the current state of research on NF-κB.
New research indicates that DNA sequence itself influences mutation rate
May 24, 2010 – Genetic variation due to DNA mutation is a driving force of adaptation and evolution, as well as a contributing factor to disease. However, the mechanisms governing DNA mutation rate are not well understood.
New lab manual for studying the biology of the nervous system in Drosophila is released
COLD SPRING HARBOR, N.Y. (May 20, 2010) - The nervous system is the most complex organ system in the human body, with circuits, synapses, and signals that control much of our physiology and behavior - both conscious and unconscious.
New book summarizes research on symmetry breaking in biology
COLD SPRING HARBOR, N.Y. (May 18, 2010) - Symmetry breaking events are critical for the survival of all living systems. They are required for cell division, development, and movement in all organisms from single-celled species to human beings. Moreover, in multicellular organisms, symmetry breaking allows the generation of cells with different fates and underpins the complex arrangement of tissues and organs achieved during embryogenesis.
New book reviews the structure and function of cell junctions
COLD SPRING HARBOR, N.Y. (May 11, 2010)- Cell-cell junctions are multi-molecular complexes that link neighboring cells. They help maintain tissue integrity, act as barriers to permeability, and allow intercellular transport.
New book surveys research on molecules that help determine cell fate during embryogenesis
COLD SPRING HARBOR, N.Y. (May 6, 2010) - Signaling by diffusible morphogens, such as Hedgehog, Wingless, TGF-, and various growth factors, is essential during embryogenesis. The establishment of concentration gradients of these morphogens is vital for developmental patterning, ensuring that distinct differentiated cell types appear in the right place and at the right time in forming tissues.
At the Helm: Leading Your Laboratory provides fresh advice for new principal investigators
COLD SPRING HARBOR, N.Y. (May 4, 2010) - Newly appointed principal research investigators have to recruit, motivate, and lead a research team, manage personnel and institutional responsibilities, and compete for funding while maintaining the outstanding scientific record that got them their position in the first place. Small wonder, then, that many principal investigators feel ill-prepared.
May’s Cold Spring Harbor Protocols Features Plant Gene Expression Methods, Xenopus Imaging
COLD SPRING HARBOR, N.Y. (Mon., May 3, 2010)- The generation of transgenic plants can be a lengthy and difficult process. Transient expression assays have been developed as faster and more convenient alternatives for investigating gene function. These assays often take advantage of the ability of Agrobacterium to transfer foreign DNA into plant cells with intact cell walls. Agrobacterium-mediated transformation is, however, inefficient and shows great variability.
microRNA network study implicates rewired interactions in cancer
May 3, 2010 - Genes interact in complex networks that govern cellular processes, much like people connect a social network through relationships. Researchers are now discovering how biological networks change and are rewired in cancer.
Second volume of groundbreaking Emerging Model Organisms series is released
COLD SPRING HARBOR, N.Y. (Apr. 29, 2010) – Mice, frogs, and E. coli are standard organisms in biology laboratories. But in the last few years, due in part to technical advances, the reduced costs of genome sequencing, and increased interest in evolution and development, the range of organisms used for research has greatly expanded.
Genome sequence of hypertensive rat expected to uncover the genetic basis of hypertension in humans
April 29, 2010 - Chronic high blood pressure, also known as hypertension, is a serious health risk factor that afflicts more than 25% of all adults worldwide, but the molecular basis of the disease remains poorly understood.
Tissue Engineering, Imaging Neuronal Circuits Featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR N.Y. (Thurs., April 1, 2010) - The goal of tissue engineering is to recapitulate healthy human organs and tissue structures in culture, and then transplant them into patients, where they are fully integrated.
Schizophrenia gene network analysis identifies age-associated defects
March 2, 2010 - The underlying causes of the debilitating psychiatric disorder schizophrenia remain poorly understood.
Protein-Bait Interactions, Display Libraries featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y. (Mon., Mar. 1, 2010) – The use of recombinant proteins, antibodies, small molecules, or nucleic acids as affinity reagents is a simple yet powerful strategy to study the protein/bait interactions that drive biological processes.
Melanoma transcriptome reveals novel genomic alterations not seen before
February 23, 2010 - Melanoma, the most deadly form of skin cancer, afflicts more than 50,000 people in the United States annually and the incidence rate continues to rise.
Method of the future uses single-cell imaging to identify gene interactions
February 9, 2010 – Cellular imaging offers a wealth of data about how cells respond to stimuli, but harnessing this technique to study biological systems is a daunting challenge.
High-Throughput Analysis of Gene Regulation, DNA Synthesis in CSH Protocols
COLD SPRING HARBOR, N.Y. (Mon., Feb. 1, 2010) – Mapping DNase I hypersensitive sites has long been the standard method for identifying genetic regulatory elements such as promoters, enhancers, silencers, insulators, and locus control regions.
New edition of popular lab manual presents latest techniques for probing cellular dynamics
COLD SPRING HARBOR, N.Y. (Jan. 15, 2009) – In recent years, substantial advances have been made in microscopy techniques, enabling biologists to understand the details of cellular structure and dynamics at a level never before possible.
Cellular Communication in the Cancer Microenvironment
In the February 1st issue of G&D, Dr. Johanna Joyce and colleagues at Memorial Sloan Kettering Cancer Center lend new insight into the mechanism by which tumor-associated macrophages promote malignant progression.
Novel Mouse Model of Demyelinating Disorder
In the February 1st issue of &D, Dr. Brian Popko (The University of Chicago) and colleagues describe how mutation of a gene called ZFP191 leads to disordered CNS myelination in mice -- reminiscent of what is seen in human multiple sclerosis (MS) patients.
New handbook for biologists who need more competence or confidence in statistics
COLD SPRING HARBOR, N.Y. (Jan. 5, 2010) – New technologies such as microarrays, next-generation sequencing, and proteomics have dramatically increased the need for quantitative reasoning among biologists when designing experiments and interpreting results. Even the most routine informatics tools rely on statistical assumptions and methods that need to be appreciated if the scientific results are to be correct, understood, and exploited fully.
Analysis of microbes, immune response featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y. (Mon., Jan. 4, 2010) – Metagenomics, the study of DNA isolated from samples of naturally occurring microbial populations, is rapidly growing. Improvements to cloning and sequencing techniques are allowing researchers to study microorganisms in environmental samples, and new knowledge of species interactions and community dynamics is emerging.
Genomes of identical twins reveal epigenetic changes that may play role in lupus
December 22, 2009 – Identical twins look the same and are nearly genetically identical, but environmental factors and the resulting cellular changes could cause disease in one sibling and not the other.
New source of classical techniques for blood-based studies in the laboratory mouse
COLD SPRING HARBOR, N.Y. (Dec. 15, 2009) - The mouse is a standard laboratory model organism, but there are currently few resources that describe conventional techniques to analyze blood and blood-forming tissues in this species. A newly released set of compact and easy-to-use laboratory resources from Cold Spring Harbor Laboratory Press fills this gap.
Live Cell Imaging Comes into Focus in the December Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y. (Tues., Dec. 1, 2009) - Live cell imaging techniques are driving a revolution in biological research. Instead of viewing dead tissues and cells fixed at a particular stage of activity, scientists can now visualize dynamic changes as they happen, permitting a better understanding of biological processes.
Genomes of biofuel yeasts reveal clues that could boost fuel ethanol production worldwide
As global temperatures and energy costs continue to soar, renewable sources of energy will be key to a sustainable future. An attractive replacement for gasoline is biofuel, and in two studies published online in Genome Research (, scientists have analyzed the genome structures of bioethanol-producing microorganisms, uncovering genetic clues that will be critical in developing new technologies needed to implement production on a global scale.
High-throughput genotyping, protein purification featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y. (Mon., Nov. 2, 2009) – The introduction of high-throughput laboratory methods has greatly increased the pace of research into the genetics of complex diseases.
Archive of renowned monograph series in molecular and cellular biology is released online
COLD SPRING HARBOR, N.Y. (Oct. 29, 2009) - Cold Spring Harbor Laboratory Press has just released the Cold Spring Harbor Monograph Archive, a complete online collection of its prestigious scholarly monographs.
MicroRNA-Mediated Metastasis Suppression
Metastases are responsible for over 90% of cancer deaths. In the upcoming issue of G&D, Dr. Robert Weinberg (MIT) and colleagues lend molecular insight into how micro-RNAs suppress tumor metastasis.
Cold Spring Harbor Protocols features RNA analysis methods
COLD SPRING HARBOR, N.Y. (Thursday, October 1, 2009) - The study of RNA has long been the tool of choice for understanding where and when genes are expressed in a cell, tissue, or organism during development or under specific physiological or environmental conditions. Recent discoveries have revolutionized our concept of RNA function; it is now known to be active in a much wider set of biological processes than was previously believed. Techniques for isolating RNA and for uncovering its interactions with proteins have taken on new importance as many laboratories define the roles of specific RNAs in the cell.
New book offers practical advice for unraveling the genetics of complex human diseases
COLD SPRING HARBOR, N.Y. (Sept. 14, 2009) - Many human diseases -- including Alzheimer’s disease, schizophrenia, cancer, and cardiovascular disease -- are caused by multiple genetic variants and the interaction of those variants with the environment.
Discovery of novel genes could unlock mystery of what makes us uniquely human
September 2, 2009 - Humans and chimpanzees are genetically very similar, yet it is not difficult to identify the many ways in which we are clearly distinct from chimps.
ChIP and vector recombineering methods featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y. (Tues., Sept. 1, 2009)- Chromatin Immunoprecipitation (ChIP) is an invaluable method for studying the interactions between proteins and DNA on a genome-wide scale. ChIP can be used to determine whether a transcription factor interacts with a candidate target gene, and is used to monitor the presence of histones with posttranslational modifications at specific genomic locations. The results are often extremely useful for investigating the functions of specific transcription factors or histone modifications.
Genome Research publishes special issue: Personal Genomes and Variation
September 1, 2009 - The September 2009 issue of Genome Research entitled "Personal Genomes and Variation" is a special issue dedicated to the burgeoning field of personal genomics.
Magnetic microbe genome attracting attention for biotech research
August 12, 2009 - The smallest organisms to use a biological compass are magnetotactic bacteria, however mysteries remain about exactly how these bacteria create their cellular magnets.
Methods for gene transfer in stem cells featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y. (Wed., Aug. 5, 2009)- Vectors derived from retroviruses are useful tools for long-term gene transfer, because they allow stable integration of transgenes and propagation into daughter cells. Lentiviral vectors are preferred because they can transduce non-proliferating cellular targets. These vectors can be engineered to target specific tissues.
New Insight into Human Ciliopathy
In the September 1st issue of G&D, Dr. Karen Oegema (UCSD) and colleagues identify the molecular basis of the lethal developmental disorder, hydrolethalus syndrome, and reveal that hydrolethalus syndrome actually belongs to the emerging class of human ciliopathy diseases.
New online publication for the molecular life sciences
COLD SPRING HARBOR, N.Y. (July 16, 2009) – Cold Spring Harbor Laboratory Press today launched a new monthly publication, Cold Spring Harbor Perspectives in Biology, that provides comprehensive, systematically structured surveys of research in exciting areas of molecular and cellular biology, genetics, developmental biology, neuroscience, cancer biology, and molecular pathology.
Microbial analysis, micropatterning methods featured in CSH Protocols
COLD SPRING HARBOR, N.Y. (Wednesday, July 1, 2009) - Microbial populations have traditionally been studied in carefully controlled, laboratory-grown cultures. New metagenomic approaches are being developed to study these organisms in environmental or medical samples.
Novel epigenetic markers of melanoma may herald new treatments for patients
June 29, 2009 - Melanoma is the most serious form of skin cancer, diagnosed in more than 50,000 new patients in the United States annually. While the rate of incidences continues to rise, survival rate has not improved and the race is on to find the genetic and cellular changes driving melanoma and to devise new means of detection and treatment.
Mobile DNA elements in woolly mammoth genome give new clues to mammalian evolution
June 9, 2009 - The woolly mammoth died out several thousand years ago, but the genetic material they left behind is yielding new clues about the evolution of mammals.
New interdisciplinary volume focuses on advances in stem cell research
COLD SPRING HARBOR, N.Y. (June 4, 2009) – In a variety of organisms, from zebrafish to fruit flies to humans, stem cells have the potential to differentiate into a variety of tissues—and, in some cases, to give rise to a complete new organism. Stem cell research, therefore, has attracted the attention of a range of biologists—reproductive biologists, cancer biologists, cell and developmental biologists, and others—who have all recognized its importance and therapeutic potential.
ChIP-Seq, Drosophila targeted mutagenesis featured in CSH Protocols
COLD SPRING HARBOR, N.Y. (Monday, June 1, 2009)- High-throughput whole-genome analysis is becoming a standard laboratory approach for investigating cellular processes. Next-generation sequencing is replacing microarrays as the technique of choice for genome-scale analysis, because it offers advantages in both sensitivity and scale.
Winners of the Genome Research poster competition at The Biology of Genomes meeting are announced.
Congratulations to Grand Prize winner, Dr. Loyal Goff, who was awarded $500 and a one year personal subscription to Genome Research for his outstanding poster presentation and to Second Prize winner, Dr. Richard Green, who won $250 and a one year personal subscription to Genome Research for his outstanding poster presentation.
Role for CISD2 Gene in Human Disease and Lifespan Control
In the May 15th issue of G&D, Dr. Ting-Fen Tsai (The National Yang-Ming University, Taiwan) and colleagues present a new animal model of human Wolfram Syndrome, and effectively link CISD2 gene function, mitochondrial integrity and aging in mammals.
New paradigm identifies gene responsible for acetaminophen-induced liver injury
May 5, 2009 - Acetaminophen (Tylenol and generics) is one of the most commonly used over-the-counter drugs in the United States. While generally safe, acetaminophen is known to cause severe liver injury if taken in high doses. But likely due to genetics, even the recommended dose can induce serious liver damage in a significant number of people.
New book summarizes current knowledge on the biology of the vertebrate skeleton
COLD SPRING HARBOR, N.Y. (May 5, 2009) – The cells, tissues, and organs that compose the skeletal system provide a supportive yet flexible framework that allows vertebrates to withstand earth's gravity yet remain mobile. Current knowledge about the vertebrate skeleton, especially recent research on skeletal development from embryo to adult, is summarized in a new monograph, The Skeletal System.
Protein analysis methods, viral vectors featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y. (Friday, May 1, 2009) – Many proteins do not function by themselves as stand-alone units. Instead, multiple proteins associate to form larger structures called protein complexes.
Genome Research publishes special issue: Genomics and Darwinism
May 1, 2009 - The May 2009 issue of Genome Research ( is a special issue celebrating the 200th anniversary of Charles Darwin's birth, and the 150th anniversary of the publication of On the Origin of Species. Published online and in print today, the issue features a collection of perspective reviews and primary research in comparative genomics, genome evolution, and population genomics.
New book, Untangling the Double Helix, explores enzymes responsible for maintaining genome integrity
COLD SPRING HARBOR, N.Y. (Apr. 15, 2009) – If it were not for a group of enzymes called topoisomerases, DNA would become a knotted, coiled, dysfunctional mess inside of a cell as it gets twisted, rolled, unzipped, and pulled by the cellular machinery that reads and copies its sequence. Topoisomerases, which are responsible for relieving this tension and maintaining the integrity of the genome, were first discovered in the 1970s by Harvard's Professor of Biochemistry and Molecular Biology James C. Wang.
Selected personal letters of Max Perutz are released in new book
COLD SPRING HARBOR, N.Y. (Apr. 9, 2009) – Nobel Prize-winning scientist Max Perutz was a campaigner for humanitarian causes, essayist, and advocate of science. A compilation of his personal letters has just been released by Cold Spring Harbor Laboratory Press. Selected and edited by his daughter Vivien Perutz, the letters in the book What a Time I am Having: Selected Letters of Max Perutz chronicle his adventurous life through his own vivid, erudite, and humorous pen.
Basic guides to PCR, labeling neurons featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y. (Wednesday, April 1, 2009) – Along with new cutting-edge methods, Cold Spring Harbor Protocols is home to an in-depth library of basic laboratory methods.
Lice genomes: Pieces of a new puzzle
March 31, 2009 - Parents and school nurses take note. Lice are a familiar nuisance around the world and vectors of serious diseases, such as epidemic typhus, in developing regions. New research indicates that lice may actually be quite unique in the animal world.
The Autophagy-Cancer Connection
In an upcoming G&D paper, Dr. Masashi Narita (Cancer Research UK) and colleagues functionally link autophagy and cellular senescence. Using a model of oncogenic ras-induced senescence, the authors show that senescence activates autophagy in human fibroblast cells.
Protein function and chromatin structure methods featured in Cold Spring Harbor Protocols
COLD SPRING HARBOR, N.Y. (Tues., March 3, 2009) – Two new methods for analyzing the roles played by proteins in cells are featured in the March issue of CSH Protocols.
Ex vivo Neural Stem Cell Expansion
In the March 1 issue of G&D, Dr. Christopher Fasano (Memorial Sloan-Kettering Cancer Center) and colleagues lend new mechanistic insight into the effective generation of neural stem cells outside of the neurogenic niche.
A little bit of spit reveals a lot about what lives in your mouth
February 27, 2009 - Like it or not, your mouth is home to a thriving community of microbial life. More than 600 different species of bacteria reside in this "microbiome," yet everyone hosts a unique set of bugs, and this could have important implications for health and disease.
Managing microRNAs
Two independent, upcoming G&D papers lend new insight into the expression of microRNAs and their targets during vertebrate development. Dr. David Bartel and colleagues describe a novel experimental system for genome-wide quantitative analysis of miRNA target expression in miRNA-expressing cells.
Camouflaging of viral DNA could be crucial step in progression of cancers
February 10, 2009 - An estimated 15% of cancer cases can be linked to a viral infection, however the biological changes that cause some asymptomatic carriers of a virus to develop full-blown tumors are not well understood.
Mosquito genes linked to insecticide resistance may be new target in fight against malaria
February 5, 2009 - Malaria remains one of the most serious diseases worldwide, claiming the lives of more than one million people per year in tropical and sub-tropical regions, the majority of whom are children under five years of age. Efforts to eliminate this mosquito-borne illness rely heavily on prevention measures, but there are growing concerns about resistance to insecticides.
CSH Protocols features stem cell differentiation, plant RNAi methods
COLD SPRING HARBOR, N.Y. (Monday, February 2, 2009) –By using OP9-DL1 cells as a support system, researchers can study the differentiation of embryonic stem cells into mature components of the immune system. This month's issue of Cold Spring Harbor Protocols ( features a set of methods from Juan Carlos Z��iga-Pfl�cker's laboratory at the University of Toronto ( detailing The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro.
Museomics yields new insights into extinct Tasmanian Tiger
January 13, 2009 - In 1902, the National Zoo in Washington D.C. arranged to have a unique and endangered animal called the thylacine, or Tasmanian Tiger, brought to the United States from Tasmania. Later that year, a female and her three cubs arrived at the zoo. However, by the mid-1930s, the thylacine was extinct, leaving behind only preserved museum specimens.
Methods for studying DNA repair and protein modification are featured in CSH Protocols
COLD SPRING HARBOR, N.Y. (Mon., Jan. 5, 2009) – This month's issue of Cold Spring Harbor Protocols ( features two articles detailing experimental methods for the analysis of molecular processes involved in DNA repair and post-translational modification of proteins.
New edition of laboratory manual includes cutting-edge techniques to study gene regulation
COLD SPRING HARBOR, N.Y. (Dec. 19, 2008) – With the growing availability of genome sequence data for a variety of organisms, many scientists are now focusing on factors that govern the expression of individual genes—an important field of molecular biology known as transcriptional regulation. A new edition of a popular laboratory manual on transcriptional regulation has just been published by Cold Spring Harbor Laboratory Press.
New manual presents robust, state-of-the-art proteomics methods for teaching and research
COLD SPRING HARBOR, N.Y. (Dec. 18, 2008) – "Life happens at the level of proteins . . . They build, process, activate, and inactivate; they polymerize, repair, support, modify, degrade, fold, migrate, and transport; they shorten, signal, cleave, inhibit, digest, fluoresce, induce, excise, carry, and repress; they bind, transfer, translocate, amplify, proofread, regulate, and perform countless more activities."
Cold Spring Harbor Protocols features tissue culture methods for immune cells and neurons
COLD SPRING HARBOR, N.Y. (Tues., Dec. 2, 2008) –The complexity of vital organ systems makes them difficult to study in living organisms. Tissue culture methods for specific cell types allow researchers to break these systems down into component parts that can be readily manipulated and observed. This month's issue of Cold Spring Harbor Protocols ( features two articles detailing experimental culture methods for cells from the immune system and the nervous system.
Viral DNA in bacterial genome could hold key to novel cystic fibrosis treatments
December 2, 2008 – The bacterium Pseudomonas aeruginosa is well known for its environmental versatility, ability to cause infection in humans, and its capacity to resist antibiotics. P. aeruginosa is the most common cause of persistent and fatal lung infections in cystic fibrosis patients. In a study published online today in Genome Research (, researchers have used genomic techniques to study a particularly virulent strain of P. aeruginosa, uncovering genetic clues to its success that will aid in the design of novel therapeutic strategies.
New Molecular Insight into Vertebrate Brain Development
In the December 1st issue of G&D, Dr. Fred H. Gage (The Salk Institute for Biological Studies) and colleagues reveal a role for the Hippo signaling pathway in the regulation of vertebrate neural development, identifying new factors - and potential therapeutic targets - that may be involved in congenital brain size disorders and neurological tumor formation.
A new world of research possibilities with Emerging Model Organisms
COLD SPRING HARBOR, N.Y. (Nov. 21, 2008) — How can moss help us to treat Alzheimer's disease? What can the lamprey immune system tell us about evolution? Can genetic studies of snapdragon populations help with efforts to conserve rare species? What can quail teach us about human aging, reproduction, and hereditary diseases? Will studies of choanoflagellates unravel the origins of animals?
Genetics of Aging and Cancer Resistance
In the November 15th issue of G&D, Dr. Kenneth Dorshkind and colleagues at the David Geffen School of Medicine (UCLA) have identified two genes - p16(Ink4a) and Arf - that sensitize lymphoid progenitor cells to the effects of aging, and confer resistance to leukemogenesis.
Cold Spring Harbor Protocols features organ and cell culture methods
COLD SPRING HARBOR, N.Y. (Mon., Nov. 3, 2008) – Understanding the function of organs like the brain, kidney and reproductive tissues requires experimental systems that allow for the study and manipulation of developing cells and tissues in the laboratory.
Scientist, academic publisher release romantic thriller set in world of biomedical science
COLD SPRING HARBOR, N.Y. (Oct. 14, 2008)—It’s a scene anyone who knows the intense, intimate world of biomedical research will recognize. Andy—diffident, driven, and close to a cancer discovery—glimpses a woman late at night in the window of a neighboring lab. Despite himself, he’s interested.
New lab manual focuses on essential methods for purifying and characterizing proteins
COLD SPRING HARBOR, N.Y. (Oct. 7, 2008) – A new, user-friendly laboratory manual for protein purification and analysis has just been released by Cold Spring Harbor Laboratory Press. Designed for routine, day-to-day use in the laboratory, it includes essential step-wise protocols as well as background information, recommended experimental strategies, and troubleshooting advice on the most fundamental protein-related methods used by scientists at all levels.
Use it or lose it? Researchers investigate the dispensability of our DNA
October 2, 2008 - Our genome contains many genes encoding proteins that are similar to those of other organisms, suggesting evolutionary relationships; however, protein-coding genes account for only a small fraction the genome, and there are many other DNA sequences that are conserved across species. What are these sequences doing, and do we really need them at all?
Emerging Model Organisms featured in CSH Protocols
COLD SPRING HARBOR, N.Y. (Wednesday, October 1, 2008) – Biological research has long relied on a small number of model organisms, species chosen because they are amenable to laboratory research and suitable for the study of a range of biological problems. However, the variety of organisms studied is currently undergoing a massive expansion,...
Brewing better beer: Scientists determine the genomic origins of lager yeasts
September 11, 2008 – Yeast, the essential microorganism for fermentation in the brewing of beer, converts carbohydrates into alcohol and other products that influence appearance, aroma, and taste. In a study published online today in Genome Research (, researchers have identified the genomic origins of the lager yeast Saccharomyces pastorianus, which could help brewers to better control the brewing process.
Interdisciplinary volume on biological rhythms serves as both primer and in-depth resource
COLD SPRING HARBOR, N.Y. (Sept. 4, 2008) – A variety of organisms—from bacteria and fungi to plants and animals—have biological rhythms, where the timing and duration of fundamental biological processes is naturally adjusted to allow them to adapt and survive, even under fluctuating environmental conditions.
Human stem cell methods featured in CSH Protocols
COLD SPRING HARBOR, N.Y. (Tuesday, September 2, 2008) – The use of human embryonic stem cells is opening new avenues for research, from the understanding of normal human development to the treatment of a wide variety of diseases.
Cold Spring Harbor Protocols highlights gene silencing, cancer cell biology methods
COLD SPRING HARBOR, N.Y. – Combining the specificity of small interfering RNA (siRNA) silencing with the versatility of lentiviral vectors gives researchers a powerful tool for the investigation of gene function both in vivo and in vitro. This month's issue of Cold Spring Harbor Protocols ( ) features a pair of protocols from Inder Verma ( and colleagues at the Salk Institute describing this method for achieving long-term down-regulation of specific target genes in a wide range of cell types.
Nucleostemin, Serotonin and Insulin Signaling: Controlling Drosophila Growth
In the July 15th issue of G&D, Dr. Matthew Scott and colleagues at Stanford University School of Medicine reveal that a protein called Nucleostemin 3 links the serotonin and insulin signaling pathways in the control of Drosophila body size.
Scientists identify genetic basis for the black sheep of the family
July 11, 2008 – Coat color of wild and domestic animals is a critical trait that has significant biological and economic impact. In a study published online in Genome Research (, researchers have identified the genetic basis for black coat color, and white, in a breed of domestic sheep.
Significant Impact Factor Boost for Scientific Journal Genome Research
COLD SPRING HARBOR, N.Y. (July 7, 2008) – Earlier this month, ThomsonReuters released the 2007 Journal Citation Reports, which includes impact factors for the world's most important scholarly journals. The scientific journal Genome Research, published by Cold Spring Harbor Laboratory Press, was reported with an impact factor of 11.224, a considerable gain over 10.256 in 2006.
HapMap browsing and DDDP methods for genetic analysis featured in CSH Protocols
COLD SPRING HARBOR, N.Y. (Tuesday, July 1, 2008) –To aid in the study of genetic diseases, scientists with the International Haplotype Map Project have developed a haplotype map of the human genome, a tool that displays common patterns of genetic variation. While data from the project are available for unrestricted public use from the project's website (, the new tools needed to browse the map can be difficult to master for the beginner.
New Molecular Insight into Amboebic Dysentery
In the June 15th issue of G&D, Dr. Sinisa Urban (Johns Hopkins University School of Medicine) and colleagues reveal a potential role for the rhomboid enzyme, EhROM1, in the pathogenesis of the enteric protozoan parasite, E. histolytica. This discovery posits EhROM1 as a prospective target in the treatment of amoebic dysentery, which affects 1/10th of the global population (~500 million people) and represents the second most common disease in the world.
A "Field Guide" to the Landscape of Cold Spring Harbor Laboratory
COLD SPRING HARBOR, N.Y. (June 9, 2008) – For decades, the beautiful grounds of Cold Spring Harbor Laboratory (CSHL), a world-renowned research institution located on the banks of Long Island Sound, have long impressed its many visitors. A new book, Grounds for Knowledge, is an attractive guide to CSHL's mix of historic and modern buildings and the striking landscape that surrounds them.
Watson-inspired innovation in research at Cold Spring Harbor Laboratory
COLD SPRING HARBOR, N.Y. (June 9, 2008) – The appointment of James Watson as Director of Cold Spring Harbor Laboratory in 1968 set off an explosive development of research at Cold Spring Harbor Laboratory (CSHL), as he recruited widely and wisely teams of investigators with diverse scientific interests. In a new book, Life Illuminated, essays by the scientists involved tell the stories of research carried out during Watson's directorship. In addition, 34 research papers published during that golden period in CSHL's history are presented in full on an accompanying CD.
Cold Spring Harbor Protocols features methods for analyzing genomes and plant cells
COLD SPRING HARBOR, N.Y. (Monday, June 2, 2008) – With the sequencing of the human genome came the startling revelation that the number of copies of a genes and other DNA sequences can vary widely between individuals. This Copy Number Variation (or CNV), contributes to our species' genetic diversity but it has also been linked to genetic diseases.
Genetics of ALS Progression
An upcoming paper from Drs. Hidenori Ichijo and Hideki Nishitoh (The University of Tokyo) and colleagues lends new and valuable insight into the genetics of ALS.
Metagenomics of skin reveals insights into the human microbiome
Friday, May 23, 2008 – The human body is home to a diverse range of microorganisms, estimated to outnumber human cells in a healthy adult by ten fold. The importance of characterizing human microbiota for understanding health and disease is highlighted by the recent launch of the Human Microbiome Project by the National Institutes of Health.
Effect of Mutant p53 Stability on Tumorigenesis and Drug Design
In the May 15th issue of G&D, Dr. Guillermina Lozano (MD Anderson Cancer Center) and colleagues reveal how the stabilization of a mutated form of p53 affects oncogenesis, and lends startling new insight into the potential pitfalls of using Mdm2 inhibitors for cancer therapy.
Watching the Clock: Generating cyanobacterial circadian rhythm
In the June 1 issue of G&D, Dr. Takao Kondo and colleagues (Nagoya University) reveal that the clock protein KaiC is the primary pacemaker for the cyanobacterial circadian clock, but not in the manner previously thought.
The FAQs about the human genome
COLD SPRING HARBOR, N.Y.– How many genes are in the human genome? Which genes are commonly associated with genetic diseases? How many mobile elements, simple sequence repeats, or protein kinases are encoded by the genome? What are the largest genes and proteins? How similar are human proteins to those of mouse, yeast, or bacteria?
Platypus genome unravels mysteries of mammalian evolution
Genome Research is publishing several papers related to analyses of the duck-billed platypus (Ornithorhynchus anatinus) genome sequence. The place of (egg-laying) monotremes, such as the platypus, in mammalian evolutionary history has remained controversial. Now, researchers are finding that the distinctive anatomical and physiological properties of the platypus are reflected in the newly sequenced platypus genome. Through comparative genomics, the platypus genome is providing remarkable insights into the evolution of venom components, the sex-determination system, testicular descent, and small RNA pathways. Primary research reports describing these novel insights will appear online May 8, concurrent with publication of the platypus genome sequence report in the journal Nature.
Cold Spring Harbor Protocols features classic approaches for analyzing chromosomes
COLD SPRING HARBOR, N.Y. (Thursday, May 1, 2008) – Recent discoveries have led to a revolution in the field of epigenetics, the study of gene regulation through the modulation of chromatin. These newly elaborated principles have brought the study of chromosomes and chromatin structure to the forefront of genetic research.
Regulating Hematopoietic Stem Cell Homeostasis and Leukemogenesis
In the April 15th issue of G&D, Dr. Richard Flavell (Yale University) and colleagues identify the c-Cbl protein as a critical repressor of hematopoietic stem cell (HSC) self-renewal. In addition to establishing a key role for protein ubiquitylation in HSC development, this finding posits c-Cbl as a potential target in research into stem cell engineering as well as cell-based leukemia treatments.
Molecular Basis for Neurodegeneration in Ataxia telangiectasia
An upcoming paper from Dr. David Wassarman (University of Wisconsin School of Medicine and Public Health) in the May 1 issue of G&D lends new insight into the pathogenesis of neurodegeneration in Ataxia telangiectasia.
Scientists develop strategy to rapidly describe outbreak strains with next-generation DNA sequencing
Friday, April 11, 2008 – In the event of an outbreak or a bioterrorist attack, rapid identification of the genetic changes responsible for virulence or drug resistance is essential to mounting an effective response. Standard DNA sequencing and analysis of a pathogen genome is time-intensive and likely impractical during an emergency. Researchers have now developed a comparative genomics strategy to drastically reduce the time needed to accurately identify unique genetic properties of a potential outbreak strain.
The story of Max Perutz, a pioneering biologist with a remarkable passion for life
COLD SPRING HARBOR, N.Y. – Max Perutz, a pioneer in the field of protein crystallography and a Nobel laureate, was one of the first to study the molecular structures of proteins. His life story, wonderfully told by Georgina Ferry, was recently published by Cold Spring Harbor Laboratory Press.
Reflecting on the social implications of human genetics research—past, present, and future
COLD SPRING HARBOR, N.Y. – In 1911, the influential geneticist Charles Davenport published Heredity in Relation to Eugenics, advancing his ideas of how genetics would improve society in the 20th century. It became a college textbook and a foundation for the widespread eugenics movement in the United States. Although the eugenic ideals of the early part of the 20th century have long been rejected, many of the issues raised by Davenport are still being debated nearly 100 years later.
A call to infuse scientific knowledge into the human experience
COLD SPRING HARBOR, N.Y. – In a provocative new book, distinguished geneticist and historian Elof Axel Carlson argues for a more scientific view of human nature, one that is based on our biology—our cellular organization, genetics, life cycle, and evolution.
Scientists reshape Y chromosome haplogroup tree gaining new insights into human ancestry
Wednesday, April 2, 2008 – The Y chromosome retains a remarkable record of human ancestry, since it is passed directly from father to son. In an article published online today in Genome Research (, scientists have utilized recently described genetic variations on the part of the Y chromosome that does not undergo recombination to significantly update and refine the Y chromosome haplogroup tree.
Cold Spring Harbor Protocols features methods to screen genomes and analyze evolution
COLD SPRING HARBOR, N.Y. (Tuesday, April 1, 2008) – Identifying genes that are important in specific tissues or processes in the mouse used to be a monumental task. New technologies and strategies have simplified this search, making it effective for even the smallest laboratories.
MicroRNAs, EMT and Cancer Progression
In the April 1 issue of G&D, a research team led by Dr. Marcus Peter (University of Chicago) identifies the microRNA miR-200 as both a powerful indicator and regulator of the epithelial phenotype of cancer cells.
Cutting edge computational molecular biology research featured in Genome Research
Genome Research is publishing several papers in coordination with the Research in Computational Molecular Biology (RECOMB) 2008 Conference, March 30, 2008 – April 2, 2008, at the National University of Singapore. Genome Research has partnered with RECOMB to publish a select number of high-quality contributions to the meeting, presenting the latest theoretical advances in computational biology and their applications in molecular biology and medicine. The papers will appear online Wednesday, March 19, 2008, and in print as a special section of the Genome Research April 2008 issue.
New Insight into the Genetics of Brain Tumor Formation
In a G&D paper published online ahead of its April 1 print publication date, Dr. William Kaelin (Dana Farber Cancer Institute) and colleagues identify a potential new neuronal tumor suppressor.
Role for MicroRNAs in Limb Regeneration
In the March 15th issue of G&D, Dr. Kenneth Poss (Duke University Medical Center) and colleagues reveal that microRNA depletion is a necessary step in tissue regeneration – a discovery with interesting implications for their use in regenerative medicine.
Growing old together: Yeast, worms, and people may age by similar mechanisms
Thursday, March 13, 2008 – A study published online today in Genome Research ( provides new insight into the evolutionary conservation of the genes and pathways associated with aging. This report describes the identification of conserved aging-related genes in simple model organisms that may lead to the characterization of similar genes playing a role in human aging and age-associated diseases.
Cold Spring Harbor Protocols features high-throughput methods for analyzing gene activity
COLD SPRING HARBOR, N.Y. (Mon., March 3, 2008) – New high-throughput methods are revolutionizing our understanding of transcriptional regulation. This month's issue of Cold Spring Harbor Protocols ( highlights two methods for analyzing the switches that turn genes on and off. Both methods are freely accessible on the Web site for Cold Spring Harbor Protocols (
Cancer theory stands the test of time – rediscovering Boveri a century later
COLD SPRING HARBOR, N.Y. – Rarely has a book in the biological sciences proved as prescient as Theodor Boveri's 1914 monograph Concerning the Origin of Malignant Tumours. Out of print for decades and previously almost impossible to track down, the book is now available as a new and authoritative translation, co-published by Cold Spring Harbor Laboratory Press and The Company of Biologists.
Tailoring Glioblastoma Therapies: One size does not fit all
An upcoming G&D paper from Dr. Azad Bonni and colleagues at Harvard Medical School lends new insight into how the unique genetic signature of glioblastoma tumors affects treatment efficacy - a finding with promising hope for the therapeutic targeting of the leading cause of cancer-related deaths in the young and middle-aged population.
Researchers identify a worldwide-distributed clone of bacteria responsible for Legionnaire's disease
Wednesday, February 6, 2008 – A study published online today in Genome Research ( ) describes new insights into Legionella pneumophila, the bacteria responsible for most cases of Legionnaires' disease. This report investigates the genetic background of L. pneumophila, provides clues to the evolution and emergence of this pathogen, and describes the identification of a worldwide-distributed epidemic clone.
RNA-based methods for developmental studies are featured in CSH Protocols
COLD SPRING HARBOR, N.Y. (Fri., Feb. 1, 2008) – This month's issue of Cold Spring Harbor Protocols ( highlights two methods to understand developmental processes in plants and flies. Both methods involve work with RNA and are freely accessible on the Web site for Cold Spring Harbor Protocols (
In vivo Visualization of Alternative Splicing
The February 1 cover of G&D features an unprecedented use of fluorescent proteins to visualize developmentally regulated alternative mRNA splicing in a living organism.
Molecular Evolution of Limb Length
In the January 15th issue of G&D, a research team led by Dr. Richard Behringer at MD Anderson Cancer Center reports that they have successfully switched the mouse Prx1 gene regulatory element with the Prx1 gene regulatory region from a bat – and although these two species are separated by millions of years of evolution -- the resulting transgenic mice displayed abnormally long forelimbs.
CSH Protocols features methods for visualizing protein dynamics
COLD SPRING HARBOR, N.Y. (Wed., Jan. 2, 2008) – This month's release of Cold Spring Harbor Protocols highlights methods that permit scientists to observe protein dynamics in chromosomes and embryos.
Making Sense of Antisense microRNAs
Three independent papers in the January 1st issue of G&D report on the discovery of a bidirectionally transcribed microRNA (miRNA) locus in Drosophila.
Link Uncovered Between Variation in Humans with Extreme Body Mass and Abnormal Splicing
Today researchers report new insights into how genetic variation may create phenotypic differences between individuals.
Cold Spring Harbor Protocols features innovative methods for embryology research
Two methods that permit scientists to examine critical stages in early embryogenesis are featured in this month's release of Cold Spring Harbor Protocols.
Anti-Aging Skin Care: Reversing skin aging by gene blockade
In the December 15th cover story of G&D, a research team led by Dr. Howard Chang (Stanford University School of Medicine) reports that the blockage of a single gene, called NF-kB, can reverse aging in the mammalian skin. This finding sets the stage for the development of future genetic age-intervention therapies.
Genetics of Hunger and Sleep
In the November 15th issue of G&D, Drs. Devanjan Sikder and Thomas Kodadek (UT Southwestern Medical Center at Dallas) identify that the protein HIF-1 mediates the regulatory effects of the neurotransmitters Orexin A and B on appetite and wakefulness.
Human RecQ Helicases, Homologous Recombination and Genomic Instability
Two independent papers in the December 1st issue of G&D detail how human RecQ helicases regulate homologous recombination and protect genome stability.
Fly Genome Tip Sheet
TIP SHEET: Highlights from papers related to the comparative analysis of twelve Drosophila genomes in Genome Research
Regulation of TATA-less Promoters
In their upcoming G&D paper, Dr. Robert Tjian (UC Berkeley) and colleagues reveal how histone gene expression is differentially regulated during Drosophila development. The researchers demonstrate that different basal transcription factors drive expression of the histone gene cluster, lending new insight into the regulation of metazoan transcription.
Domestic Cat Genome Sequenced
A report that appears in the scientific journal Genome Research ( ) details the first assembly, annotation, and comparative analysis of the domestic cat genome (Felis catus).
Scientists Discover New Genetic Variant Associated with Prostate Cancer in African Americans
Wednesday, October 31, 2007 – Today researchers report a newly identified genetic variation that is linked to higher incidence of prostate cancer in African American men. This study, which emphasizes the importance of characterizing genetic markers associated with prostate cancer in high-risk populations, is published online in Genome Research ( ).
3-D Model of Esophageal Cancer
In the November 1st issue of G&D, a team of scientists led by Dr. Anil Rustgi (UPENN) presents an innovative new model of esophageal cancer, which holds great promise as an experimental platform to investigate the etiology and possible treatment of this devastating disease.
Cold Spring Harbor Protocols highlights a method that captures cell growth and activity.
COLD SPRING HARBOR, N.Y. (Thurs., Nov. 1, 2007)– This month's issue of Cold Spring Harbor Protocols ( features a cutting-edge method that provides a snapshot of growth and activity patterns in mixed populations of cells.
The Genetics of MLL Leukemogenesis
In the November 1st issue of G&D, Dr. Michael Cleary (Stanford University School of Medicine) and colleagues identify the gene Meis1 as a critical player in the establishment of leukemia stem cells, and the development of MLL leukemia.
Regulating Embryonic Stem Cell Self-Renewal
In the October 15th issue of G&D, Dr. Huck-Hui Ng and colleagues at the Genome Institute of Singapore identify two genes – called Jmjd1a and Jmjd2c - that regulate self-renewal in embryonic stem cells. This finding will have important ramifications for embryonic stem cell research.
Patterning the Mammalian Brain
In a paper published online ahead of its October 15th print date, Dr. Aaron DiAntonio (Washington University) and colleagues reveal that Phr1 – the sole mammalian ortholog of the invertebrate ubiquitin ligase genes highwire (in Drosophila) and rpm-1 (in C. elegans) – also plays a crucial role in sculpting the mammalian nervous system, albeit in a distinctly different manner.
The hottest jobs in science, and how to land them
A new book presents a comprehensive overview of careers in the biotechnology and pharmaceutical industry.
Brains to Behavior
COLD SPRING HARBOR, N.Y. (Mon., Oct. 1, 2007) – Research in the field of neuroscience is constantly expanding to provide knowledge about biological mechanisms that underlie our ability to experience and interact with the world around us. To facilitate such research, two neuroscience methods are featured in this month's release of Cold Spring Harbor Protocols. Both are freely accessible online and include movie clips that help to illustrate the procedures.
Venous Origin of the Mammalian Lymphatic Vasculature
In the October 1 issue of G&D, Dr. Guillermo Oliver (St. Jude Children's Research Hospital) and colleagues present new evidence to resolve a century-old debate over the origin of the mammalian lymphatic vasculature. Understanding the development of the lymphatic vasculature is integral to understanding its function in both health (mediating immunity and maintaining tissue fluid levels) and disease (lymphedema and spreading tumor metastasis).
Genetics of Imatinib Resistance in ALL
In the September 15th issue of Genes & Development, Drs. Richard T. Williams, Willem den Besten, and Charles J. Sherr at Howard Hughes Medical Institute, St. Jude Children's Research Hospital in Memphis TN, lend new insights into how an aggressive form of acute lymphoblastic leukemia (ALL) develops, and how sensitivity to the targeted chemotherapeutic drug, imatinib, can be diminished through interactions between tumor cells and the host microenvironment.
Modeling Skin Cancer
COLD SPRING HARBOR, N.Y. (Tues., Sept. 4, 2007) – Skin cancer is the most prevalent form of human cancer in the United States, according to the Centers for Disease Control. But in order to more fully understand skin cancer in humans, scientists must use model organisms, such as mice, to study the disease in the laboratory.
tDNAs, RNA pol III and chromatid cohesion
In the September 1st issue of G&D, Drs. Rudra Dubey and Marc Gartenberg (UMDNJ) reveal a surprising new role for tDNAs and RNA polymerase III-associated proteins in sister chromatid cohesion.
Scientists identify novel mode of transcriptional regulation
In an upcoming issue of G&D, Drs. Maria Divina Deato and Robert Tjian (HHMI, UC Berkeley) reveal that the formation of an alternative transcriptional core promoter complex directs cell-type specific differentiation during myogenesis.
Ago2 and Hematopoiesis
Argonaute 2 (Ago2) is unique among its family: It is the only one of the four mammalian Argonaute proteins that exhibits endonuclease "slicer" activity (facilitation of miRNA-guided cleavage of target mRNA).
Diabetes Release
Monday, August 13, 2007 – Today, researchers report for the first time that genetic variants in mitochondria—energy-producing structures harboring DNA that are inherited only from the mother—are directly linked to metabolic markers for type 2 diabetes.
MicroRNA Conflict Resolution
In the August 1st issue of G&D, a team of Japanese scientists led by Dr. Shigeyuki Yokoyama (RIKEN and the University of Tokyo), presents heretofore the most relevant experimental system of microRNA-mediated translational repression.
Gene Silencing
COLD SPRING HARBOR, N.Y. (Wed., Aug. 1, 2007) – Nearly a decade ago, now-Nobel laureates Craig Mello and Andrew Fire discovered that they could insert short RNA molecules into worms and shut down specific genes. Today, scientists routinely use this powerful method, termed RNA interference, to study the functions of specific genes in mammalian systems.
Genomics study
AURORA, Colo. (Tues., July 31, 2007) – Today, researchers from the University of Colorado Health Sciences Center, along with colleagues from Stanford University, report the results of a large-scale, genome-wide study to investigate gene copy number differences among ten primate species, including humans. The study provides an overview of genes and gene families that have undergone major copy number expansions and contractions in different primate lineages spanning approximately 60 million years of evolutionary time.
Toxic Milk: Scientists find key protein affecting quality of maternal-infant
In the August 1 issue of G&D, Dr. Ronald Evans (Salk Institute) and
colleagues report on their discovery that mutations in the mouse gene
encoding PPARg adversely affect lactation milk quality, and have serious
health consequences for nursing pups.
The Flip Side of p27
In a paper published online ahead of its July 15th print date, Dr. James Roberts (FHCRC) and colleagues reveal a surprising new role for the p27 tumor suppressor in tumors and stem cells.
On Target
In an upcoming Genes & Development paper, Dr. Christopher Counter and colleagues at the Duke University Medical Center have identified IL6 as a new target in the battle against Ras-induced cancers.
Genes & Development ranked #1 in the field of Developmental Biology according to
Bioinformatics-related methods
COLD SPRING HARBOR, N.Y. (Mon., July 2, 2007) – Large-scale undertakings such as the Human Genome Project have produced massive amounts of data. To make sense of it all, powerful mathematical and statistical algorithms were developed, resulting in the interdisciplinary field called "bioinformatics."
Role of Autophagy in Tumorigenesis
In the upcoming issue of G&D, Dr. Eileen White and colleagues at Rutgers University/University of Medicine and Dentistry of New Jersey/Cancer Institute of New Jersey, report, for the first time, that the cellular self-digesting process of autophagy can protect genome integrity – lending new insight into the seemingly contradictory roles of autophagy as both a cell survival and tumor suppressor pathway.
Regulating Alternative Splicing During Neural Development
In the July 1st issue of Genes & Development, Dr. Douglas Black (UCLA) and colleagues detail how alternative splicing is reprogrammed during neuronal development.
Endosome-Mediated Signaling in Plants
In a paper that will be published online in advance of its July 1st publication date, Drs. Niko Geldner, Joanne Chory and colleagues (The Salk Institute and HHMI) demonstrate that endosomes can function as signaling platforms in plants, as well as in animals.
On the Defense
The innate immune response is the body’s first line of defense against pathogen infection. In the June 15th issue of G&D, Dr. Xin Li (University of British Columbia) and colleagues report that three proteins work together in the MOS4-associated complex (MAC) to execute innate immunity in the mustard weed, Arabidopsis.
Since the completion of the Human Genome Project in 2003, research efforts have been aimed at analyzing the functions of various sequences in the genome, using both experimental and computational strategies. The June issue of Genome Research ( is devoted to The ENCODE (ENCyclopedia Of DNA Elements) Project, whose goal is to characterize all functional elements in the human genome.
Pediatric Cancer Stem Cell Identified
In a G&D report that will be published online ahead of its June 1 issue date, Dr. Leonard Zon (Children's Hospital Boston) and colleagues have identified the cancer stem cell for rhabdomyosarcoma, the most common soft-tissue sarcoma of childhood.
Embryos, clones, stem cells
COLD SPRING HARBOR, N.Y. (Fri., June 1, 2007) – Cloning, X-chromosome inactivation, stem cells, and embryogenesis are hot areas of research at the moment, and protocols featured in this month's release of Cold Spring Harbor Protocols ( will aid these studies.
The Yin and the Yang of B-cell Development
A new paper in the May 15th issue of Genes & Development reveals how a protein called Yin Yang 1 regulates early B cell development.
Unicellular microRNA Discovery
In the May 15th issue of Genes & Development, an international collaboration of researchers, led by Dr. Yijun Qi (National Institute of Biological Sciences, China), report on their discovery of microRNAs in the unicellular green alga, Chlamydomonas reinhardtii. This is the first finding of microRNAs in a unicellular organism.
Opossum Tip Sheet
Genome Research is publishing three papers related to the genome of the gray short-tailed opossum, Monodelphis domestica, a small, nocturnal marsupial found in South America.
MicroRNAs as Tumor Suppressors
In the May 1st issue of Genes & Development, Drs. Yong Sun Lee and Anindya Dutta (UVA) reveal that microRNAs can function as tumor suppressors in vitro.
Methods for labeling DNA and protein molecules with various colorful (and not-so-colorful) tags
A rainbow of methods promises insights into biological processes and diseases. This month's release of Cold Spring Harbor Protocols features freely available methods for marking molecules to identify gene alterations and metabolic shifts.
ATR Checkpoint-Activating DNA Structure
As published in the April 15th issue of Genes & Development, Dr. Karlene Cimprich and colleagues at Stanford University have determined the minimal DNA structure sufficient to activate the ATR-mediated DNA damage checkpoint.
Reliable methods for gene and protein analyses
This month's release of Cold Spring Harbor Protocols highlights methods for creating and detecting specific proteins, as well as for characterizing the activities of specific genes during embryonic development.
New Therapeutic Insight into Duchenne Muscular Dystrophy
In the April 1st issue of Genes & Development, Dr. Bruce Spiegelman (Dana Farber Cancer Institute) and colleagues identify a key genetic component of and possible therapeutic target for Duchenne muscular dystrophy.
Scientists identify a candidate gene for osteoporosis
LOMA LINDA, Calif. (Thurs., Mar. 29, 2007) – Today, researchers report the identification of a gene that may play a role in susceptibility to osteoporosis—the crippling disease that leads to bone fractures, especially of the hip and spine. The study, conducted by scientists at the Musculoskeletal Diseases Center of the Jerry L Pettis Memorial Veteran's Affairs Medical Center at Loma Linda, shows convincing evidence that a gene called DARC negatively regulates bone density in mice.
Gene Atp5a1 may suppress colorectal cancer
PHILADELPHIA (Thurs., Mar. 22, 2007) – In today's online edition of Genome Research, a husband-and-wife research team from Thomas Jefferson University report the discovery of a gene that, when mutated, may suppress colorectal cancer.
Ras signaling and Lung Cancer
In the March 15th issue of Genes & Development, Dr. Tyler Jacks (MIT) and colleagues lend new insight into the contribution that the ras oncogene makes to developmental disorders and cancer.
Maternal microRNAs
Two new papers in the March 15th issue of Genes & Development explore the role of microRNAs in early mammalian development. Two independent research groups, led by Dr. Gregory Hannon (CSH Laboratory) and Drs. M. Azim Surani (Wellcome Trust) and Kaiqin Lao (Applied Biosystems), compared cytological characteristics, microRNA profiles and gene expression data between Dicer-deficient and wild-type mouse oocytes.
Fruit Fly Methods
COLD SPRING HARBOR, N.Y. (Thurs., Mar. 1, 2007) – For the past century, fruit flies—or Drosophila—have provided innumerable insights into the genetics and biology of development, learning and memory, behavior, vision, and other processes. But for researchers who conduct these studies, the logistics of housing and feeding the hundreds or thousands of flies needed for experiments can be daunting. To address this concern, the current issue of Cold Spring Harbor Protocols—released online today (—includes a series of articles for maintaining and manipulating flies in the laboratory.
New Hope for Regenerative Medicine
In the February 15th issue of G&D, Dr. K. John McLaughlin and colleagues report on their success in using uniparental embryonic stem cells to replace blood stem cells in mice.
Modeling Lung Cancer
In a new report in the February 15th issue of G&D, Dr. Martin McMahon (UCSF) and colleagues present a novel mouse model of non-small cell lung cancer, which will serve as a useful tool to test the efficacy of novel chemotherapeutic drug therapies in the early stages of lung tumorigenesis.
Lighting up life
COLD SPRING HARBOR, N.Y. (Thurs., Feb. 1, 2007) – Just over a decade ago, biologists isolated a unique protein from jellyfish that could be inserted into other organisms—from E. coli to pigs—and cause them to radiate a brilliant green color. This green fluorescent protein (GFP) has allowed biologists to make many new discoveries regarding how living cells function. But one kingdom of life—plants—has presented special challenges to GFP detection: plants harbor tough cell walls and enormous subcellular structures that interfere with visualization, and their natural green pigments can mask the luminescent qualities of GFP.
Screening for Complex Genetic Interactions
In a report published online ahead of the January 15th print edition, Dr. David Amberg (SUNY Upstate Medical University) and colleagues have developed a large-scale reverse genetic screen to identify complex haploinsufficient interactions in S. cerevisiae.
Imaging techniques permit scientists to follow a day or four in the life of a cell
COLD SPRING HARBOR, N.Y. (Tues., Jan. 2, 2007) – The movement and growth of cells are critical for normal physiological processes, and—when perturbed—can result in negative outcomes such as tumor formation. Understanding how live cells function is therefore invaluable for molecular and cellular biologists, and advanced techniques to visualize cells in action are of great importance.
Counter Defense Strategy of Virus
RNA silencing evolved as a means of defense against viral pathogens. In turn, viruses have evolved a counter-defense mechanism to inhibit RNA silencing. In the December 1st issue of G&D, a team of NYC scientists, led by Dr. Nam-Hai Chua at the Rockefeller University, lend new insight into how the Cucumber mosaic virus (CMV) executes its counter-defense.
Firestorms - Hicks, Wigler

COLD SPRING HARBOR, N.Y. (Fri., Dec. 1, 2006) – The first high-resolution analysis of genomic alterations in breast tumors is reported today in the scientific journal Genome Research.

Plants, plasmids and possibilities
COLD SPRING HARBOR, N.Y. (Fri., Dec. 1, 2006) – Decaffeinated coffee plants, pest-resistant cotton, and Vitamin A-producing rice varieties have all been developed by introducing genes into plants. Scientists also create modified plants to identify and characterize the functions of specific genes. The current issue of Cold Spring Harbor Protocols—released online today (—includes a set of techniques for the creation of transgenic plants.
New technology used to construct the first map of structural variation in the human genome

Thurs., Nov. 23, 2006 – Beyond the simple stream of one-letter characters in the human genome sequence lies a complex, higher-order code. In order to decipher this level of architecture, scientists have developed powerful new experimental and algorithmic methods to detect copy number variants (CNVs)—defined as large deletions and duplications of DNA segments.

Cells, dyes and videotape: Online scientific methods journal incorporates multimedia
COLD SPRING HARBOR, N.Y. (Fri., Nov. 3, 2006) – Observing the microscopic mysteries of embryos, cells, and chromosomes is feasible with advanced live imaging technologies. In space and time, researchers can follow the fates of embryos, track migrating cells, and watch how molecules signal and interact with each other—all in their native environments. The current issue of CSH Protocols, released online today (, includes biomedical research techniques that incorporate this 'cellular cinematography' and—for the first time—adds multimedia content in the form of movie clips.
Phoenix Rising - Scientists resuscitate a 5 million-year-old retrovirus
VILLEJUIF, France (Tues., Oct. 31, 2006) – A team of scientists has reconstructed the DNA sequence of a 5-million-year-old retrovirus and shown that it is able to produce infectious particles.
Honey Bee - Beye
Genome Research has devoted this month's issue to studies that provide insight into the biology of the honey bee (Apis mellifera).
The Neurobiology of Amnesia
Cold Spring Harbor, N.Y., Mon., Oct. 3, 2006—A first kiss, an exotic vacation, a sports team championship, a child's first words: all are memorable events. But when someone has amnesia, have the memories been completely purged from the brain or are they simply irretrievable? Is amnesia a defect in memory storage, or memory recovery?
CSH Protocols publishes cutting-edge methods for analyzing complex molecular interactions
Cold Spring Harbor, N.Y., Tues., Oct. 3, 2006 – With the genomes of hundreds of organisms now catalogued, one of the next major challenges is to identify proteins and their interactions. The current issue of CSH Protocols, published online (, features two freely available, cutting-edge methods that address this challenge.
Cold Spring Harbor Protocols features methods for analyzing protein interactions
Cold Spring Harbor, N.Y., Fri., Sept. 8, 2006 – Cold Spring Harbor Protocols, an online journal that publishes methods used in a wide range of biology laboratories, has added over 40 new peer-reviewed protocols to its archive today. The new collection highlights two techniques for characterizing protein interactions, which will aid many cell and molecular biologists—including those who seek to identify the molecular basis of human diseases. Both of these methods are freely accessible from the journal's website:
Loring and Fan

San Diego, Calif. -- Scientists from the Burnham Institute for Medical Research (BIMR) and Illumina Inc., in collaboration with stem cell researchers around the world, have found that the DNA of human embryonic stem cells is chemically modified in a characteristic, predictable pattern.

DNA to the Defense
CAMBRIDGE, U.K., Fri., Aug 4, 2006 – A team of scientists headed by Dr. Sara Melville at the University of Cambridge has shown that the parasite known to cause African sleeping sickness has evolved an unusual chromosomal structure as a result of environmental adaptation.
RNA interference methods
Cold Spring Harbor, N.Y., Tues., Aug. 1, 2006 – The current issue of Cold Spring Harbor Protocols, published online today, features new, freely available methods for using RNA interference (RNAi) in mice and Drosophila.
Potato blight pathogenicity explained
WAGENINGEN, The Netherlands, Mon., July 3, 2006 – A team of researchers from Wageningen University report in this month's issue of Genome Research that they have identified a unique genetic fingerprint in the pathogen responsible for potato blight.
New Development for reccessive genetic disorder carriers
PHILADELPHIA, Mon., July 3, 2006 – Scientists Vivian Cheung and Warren Ewens from the University of Pennsylvania have developed a new approach for the diagnosis of medical disorders that are inherited in a recessive manner.
Lurking lung cancer alleles
LONDON, U.K., Thurs., June 1, 2006 – In the largest genome-wide scan for lung cancer-susceptibility genes to date, scientists from The Institute of Cancer Research have identified 64 gene variants that may predispose some individuals to lung cancer.
Hotspots - Drs. Ohno, Miura and others
FUKUOKA, Japan, Mon., May 1, 2006 – In this month's issue of the leading scientific journal Genome Research, scientists from Kyushu University report how environmentally damaged DNA may contribute to human genetic diversity.
Major obesity gene is lost in the shuffle
TOKYO, Japan, Sat., April 1, 2006 – Scientists from The University of Tokyo announce today that gibbons, arboreal primates that inhabit the jungles of Southeast Asia, do not carry a major obesity gene that is present in the genomes of all other primates, including humans.
Where jumping genes fear to tread
BRISBANE, Australia – Scientists from the University of Queensland report in the journal Genome Research that large segments of the human genome are conspicuously devoid of ubiquitous mobile DNA elements called transposons. The locations of these regions are highly conserved among mammalian species and are enriched in genes crucial for the regulation of developmental processes.
Tandem Transcripts Team Together
In the January issue of the journal Genome Research, two teams of scientists describe a widespread phenomenon in the human genome called transcription-induced chimerism (TIC), where two adjacent genes produce a single, fused RNA transcript.
Pregnant protein-coding genes carry RNA babies
BEIJING, China – Scientists from the Chinese Academy of Sciences have performed a comprehensive analysis of small, non-protein-coding RNAs in the model nematode, C. elegans. They characterize 100 heretofore-undescribed transcripts, including two novel classes; they provide insights into the genomic structure and transcriptional regulation of non-coding RNAs; and they underscore the importance of non-coding RNAs in nematode development.
Primate Tip Sheet

The September 2005 issue of Genome Research presents a series of studies that provide insight into the evolution and variation of primate genomes.

HEIDELBERG, Germany, Mon., February 14, 2005 – A team of scientists led by Peer Bork, Ph.D., Senior Bioinformatics Scientist at the European Molecular Biology Laboratory, report today in the journal Genome Research that they have identified a new primate-specific gene family that spans about 10% of human chromosome 2.
Chicken Genomic Sequence
COLD SPRING HARBOR, N.Y., Thurs., Dec. 9, 2004 – Studies published online today in the journal Genome Research ( utilize the chicken genomic sequence, in comparison with genomic sequences from other species, to illuminate several interesting aspects of vertebrate evolution.
Living Fossil - coelacanth
STANFORD, Calif., Mon., Nov. 15, 2004 – A team of Stanford University researchers led by Richard Myers, Ph.D., in collaboration with Chris Amemiya, Ph.D., of the Benaroya Research Institute in Seattle, campaign in the December issue of Genome Research for deciphering the genetic code of a "living fossil" fish, the coelacanth.
September 27, 2004 Cold Spring Harbor Laboratory Press announced today that authors of papers in its journal Genome Research can now choose to have their papers made freely available online immediately upon publication.

A National Institute of Genetics news release on an article that was published in the April 15, 2017 issue of Genes & Development